Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta

Abstract

The regulation of lipid metabolism is complex, and is currently an extensive area of research. In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned from the liver of Synechogobius hasta, including g6pd, 6pgd, me, icdh, fas, accα, accβ, lpl, atgl, hsla, hslb, cpt 1a, srebp‐1, pparα, and pparγ. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of S. hasta. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at varying levels. Practical applications: Excessive fat accumulation and disordered lipid metabolism have become serious problems in the sustainable and healthy development of aquaculture. The present study facilitates studies on the regulation of lipid metabolism at the molecular level in fish. The tissue expression profiles of genes increase our understanding of their physiological roles. In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned and characterized from Synechogobius hasta, including g6pd, 6pgd, me, icdh, fas, accα, accβ, lpl, atgl, hsla, hslb, cpt 1a, srebp‐1, pparα, and pparγ. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of S. hasta. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at the varying levels.

Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

“…Furthermore, high-lipid diets may lead to excessive lipid deposition and metabolic disturbances in organisms (3) . Lipid metabolism includes lipid uptake, transport, anabolism and catabolism and involves several biochemical pathways with key enzymes and transcriptional factors (4) . Moreover, lipid is transported between tissues mainly in the form of lipoproteins (5) .…”

Effects of Dietary Carbohydrate to Lipid Ratios on Growth Performance, Muscle Fatty Acid Composition, and Intermediary Metabolism in Juvenile Black Seabream (Acanthopagrus schlegelii)

“…The lipid metabolism in the liver is a very complex process; hepatocytes not only import and export lipids via lipoprotein, but they also oxidize lipids through fatty acid oxidation or synthesize new lipid by de novo lipogenesis (Akie and Cooper, 2015). It is generally reported that fas is the significant lipogenic enzyme for the anabolic alteration of dietary carbohydrates to fatty acids (Chen et al, 2015), and accα is reflected as an important enzyme in the synthesis of long-chain poly-unsaturated fatty acids (Qian et al, 2015;Castro et al, 2016). In the present study, both fas and accα played a vital role in fatty acid biosynthesis.…”

Regulation of Dietary Lipid Sources on Tissue Lipid Classes and Mitochondrial Energy Metabolism of Juvenile Swimming Crab, Portunus trituberculatus

“…Another reason was that the high expression levels of genes involved in anabolism, such as fas , acc , 6pgd , g6pd , and srebp-1 (Ayisi et al, 2018). Some studies demonstrated that FAS is the main lipogenic enzyme which catalyzes successive condensation reactions to form a fatty acid, playing a key role in energy homeostasis (Chen et al, 2015). ACC is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid biosynthetic pathway (Barber et al, 2005).…”