Molecular cloning and yeast two‐hybrid provide new evidence for unique sporophytic self‐incompatibility system of <i>Corylus</i>

Hou, Sen; Zhao, Tengteng; Yang, Zhen; Yang, Dan; Li, Q; Liang, Lisong; G, Wang; Ma, Qinghua

doi:10.1111/plb.13347

Cited by 3 publications

(4 citation statements)

References 77 publications

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“…It is believed that the SRK-SCR recognition via phosphorylation and degradation facilitates the pollen‒pistil interaction ( Gu et al, 1998 ; Goring et al, 2014 ) and the SI signal transducers include SRK, SLG, SCR/SP11, and MLPK ( Takasaki et al, 2000 ; Takayama et al, 2000 ; Murase et al, 2004 ; Kakita et al, 2007b ; Kitashiba and Nasrallah, 2014 ). Nevertheless, SRK-ARC1 interaction was not functioning in Corylus , indicating that the SSI system was distinct between Corylus and Brassica ( Hou et al, 2022 ). Additionally, the Fagopyrum genome did not contain SRK or SLG ( Banović et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative stigmatic transcriptomics reveals self and cross pollination responses to heteromorphic incompatibility in Plumbago auriculata Lam.

Hu,

Lin,

et al. 2024

Front. Genet.

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“Heteromorphic self-incompatibility” (HetSI) in plants is a mechanism of defense to avoid self-pollination and promote outcrossing. However, the molecular mechanism underlying HetSI remains largely unknown. In this study, RNA-seq was conducted to explore the molecular mechanisms underlying self-compatible (SC, “T × P” and “P × T”) and self-incompatible (SI, “T × T” and “P × P”) pollination in the two types of flowers of Plumbago auriculata Lam. which is a representative HetSI plant. By comparing “T × P” vs. “T × T”, 3773 (1407 upregulated and 2366 downregulated) differentially expressed genes (DEGs) were identified, 1261 DEGs between “P × T” and “P × P” (502 upregulated and 759 downregulated). The processes in which these DEGs were significantly enriched were “MAPK (Mitogen-Activated Protein Kinases-plant) signaling pathway”, “plant-pathogen interaction”,“plant hormone signal transduction”, and “pentose and glucuronate interconversion” pathways. Surprisingly, we discovered that under various pollination conditions, multiple notable genes that may be involved in HetSI exhibited distinct regulation. We can infer that the HetSI strategy might be unique in P. auriculata. It was similar to “sporophytic self-incompatibility” (SSI) but the HetSI mechanisms in pin and thrum flowers are diverse. In this study, new hypotheses and inferences were proposed, which can provide a reference for crop production and breeding.

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Section: Discussionmentioning

confidence: 99%

Comparative stigmatic transcriptomics reveals self and cross pollination responses to heteromorphic incompatibility in Plumbago auriculata Lam.

Hu,

Lin,

et al. 2024

Front. Genet.

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“…We observed secretion and pollen adhesion on the stigma surface and blue fluorescence on the papillae within 60 min after compatible and incompatible pollination. While in incompatible pollination, bunched, short, or bulbous pollen tubes which do not penetrate the stigmatic surface were observed after 4 h ( Li, 2020 ; Hou et al, 2022 ). These observations suggested that pollen adhesion, hydration, and penetration of pollen tube are the earliest steps blocked in self-incompatible pollination.…”

Section: Discussionmentioning

confidence: 99%

“…However, neither is located on LG5. Recently, also from “ Dawei ,” Hou et al (2022) identified five genes ( ChaTHL1 , ChaTHL2 , ChaMLPK , ChaARC1 , and ChaEX70A1 ), homologous to those participating in the downstream SSI response of Brassica . However, the expression and two-hybrid yeast assay analyses suggested the downstream signaling-pathway (SRK–ARC1 interaction) as the Brassica SSI response was not conserved in Corylus .…”

Section: Introductionmentioning

confidence: 99%

Stigmatic Transcriptome Analysis of Self-Incompatible and Compatible Pollination in Corylus heterophylla Fisch. × Corylus avellana L.

et al. 2022

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Self-incompatibility (SI) protects plants from inbreeding depression due to self-pollination and promotes the outcrossing process to maintain a high degree of heterozygosity during evolution. Corylus is an important woody oil and nut species that shows sporophytic SI (SSI). Yet the molecular mechanism of SI in Corylus remains largely unknown. Here we conducted self- (“Dawei” × “Dawei”) and cross-pollination (“Dawei” × “Liaozhen No. 7”) experiments and then performed an RNA-Seq analysis to investigate the mechanism of pollen–stigma interactions and identify those genes that may be responsible for SSI in Corylus. We uncovered 19,163 up- and 13,314 downregulated genes from the comparison of different pollination treatments. These differentially expressed genes (DEGs) were significantly enriched in plant–pathogen interaction, plant hormone signal transduction, and MAPK signaling pathway–plant. We found many notable genes potentially involved in pollen–stigma interactions and SSI mechanisms, including genes encoding receptor-like protein kinases (RLK), calcium-related genes, disease-resistance genes, and WRKY transcription factors. Four upregulated and five downregulated DEGs were consistently identified in those comparison groups involving self-incompatible pollination, suggesting they had important roles in pollen–pistil interactions. We further identified the S-locus region of the Corylus heterophylla genome based on molecular marker location. This predicted S-locus contains 38 genes, of which 8 share the same functional annotation as the S-locus genes of Corylus avellana: two PIX7 homologous genes (EVM0002129 and EVM0025536), three MIK2 homologous genes (EVM0002422, EVM0005666, and EVM0009820), one aldose 1-epimerase (EVM0002095), one 3-dehydroquinate synthase II (EVM0021283), and one At3g28850 homologous gene (EVM0016149). By characterizing the pistil process during the early postpollination phase via transcriptomic analysis, this study provides new knowledge and lays the foundation for subsequent analyses of pollen-pistil interactions.

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“…Several genes encoding RLKs have been identified at the S -locus (e.g., MIK2 and Putative Interactor of XopAC 7 [ PIX7 ]) ( Hill et al., 2021 ; Hou et al., 2022a ). Expression analyses and yeast two-hybrid assays confirmed that Corylus does not employ the SRK-mediated signaling pathway of Brassica SSI ( Hill et al., 2021 ; Hou et al., 2022a , 2022b ). Recent phylogenetic analysis provided additional evidence supporting the genus-specific evolution of SSI in Corylus , which is not shared by other members of Betulaceae ( Brainard et al., 2023 ).…”

Section: Molecular Basis Of Self-pollen Rejection In Different Si Sys...mentioning

confidence: 90%

Molecular insights into self-incompatibility systems: From evolution to breeding

Zhang,

Li,

Zhao

et al. 2024

Plant Communications

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Molecular cloning and yeast two‐hybrid provide new evidence for unique sporophytic self‐incompatibility system of Corylus

Abstract: Ping'ou hybrid hazelnut (C. heterophylla Fisch. 9 C. avellana L.); pistil-pollen interactions; quantitative real-time PCR (qRT-PCR); RACE cloning; yeast two-hybrid.

Cited by 3 publications

References 77 publications

Comparative stigmatic transcriptomics reveals self and cross pollination responses to heteromorphic incompatibility in Plumbago auriculata Lam.

Comparative stigmatic transcriptomics reveals self and cross pollination responses to heteromorphic incompatibility in Plumbago auriculata Lam.

Stigmatic Transcriptome Analysis of Self-Incompatible and Compatible Pollination in Corylus heterophylla Fisch. × Corylus avellana L.

Molecular insights into self-incompatibility systems: From evolution to breeding

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