Molecular cloning, characterisation, and tissue distribution of oestrogen receptor alpha in eelpout (Zoarces viviparus)

Andreassen, Troels T.; Skjoedt, Karsten; Anglade, Isabelle; Kah, Olivier; Korsgaard, Bodil

doi:10.1016/s0016-6480(03)00101-1

Cited by 46 publications

(29 citation statements)

References 73 publications

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“…Therefore, the main target cells of E 2 via ERs in Atlantic cod testicular cells could be Leydig cells, and ERs (particularly in esr1 and esr2a) may be involved in growth and maturation of Leydig cells to maintain their steroidogenesis. Nevertheless, there are reports of results conflicting with our observations, which show predominant localization of esr in fish mitotic or meiotic germ cells in eelpout (Andreassen et al 2003) and channel catfish (Wu et al 2001), indicating a diversity of E 2 signaling requirements of spermatogenic cells among teleosts. There are only two other reports describing the localization of esrs in teleost ovarian cells so far: esr1 in eelpout (Andreassen et al 2003) and esr1, esr2a, and esr2b in medaka (Chakraborty et al 2011b).…”

Section: Discussioncontrasting

confidence: 99%

“…In teleosts, there is very limited knowledge about the localization of ER mRNA or protein in adult gonads and their expression pattern is controversial (Bouma & Nagler 2001, Wu et al 2001, Andreassen et al 2003, Chakraborty et al 2011b). This study delivers significant insights into this matter by identifying the cells expressing esr paralogs in fish gonads for both sexes.…”

Section: Discussionmentioning

confidence: 99%

“…Despite the paramount importance of estrogen signaling mediated by ERs in fish gonadal development, information about the precise localization of ERs is still limited to a few fish species: ERa in the interstitial fibroblasts (the Leydig cell precursors) in testis of rainbow trout Oncorhynchus mykiss (Bouma & Nagler 2001), ERa in thecal, granulosa, and interstitial cells in ovary and in Sertoli cells in testis of eelpout Zoarces viviparus (Andreassen et al 2003), and ERa in granulosa cells in ovary and ERb2 in cells in undifferentiated gonad in medaka Oryzias latipes (Chakraborty et al 2011a). Therefore, information about localization patterns and expression profiles for ER subtypes in teleost gonadal cells is required in order to understand their potential role in regulating gene transcription and gonadal development process in teleosts.…”

Section: Introductionmentioning

confidence: 99%

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Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle

Nagasawa

Presslauer

Kirtiklis

et al. 2014

Journal of Molecular Endocrinology

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The role of sex steroid regulation in gonadal maturation is a very complex process that is far from being fully understood. Hence, we have investigated seasonal changes in gonadal expression of estrogen receptors (ERs) in Atlantic cod (Gadus morhua L.), a batch spawner, throughout the annual reproductive cycle. Three nuclear ER partial cDNA sequences (esr1, esr2a, and esr2b) were cloned and all esr transcripts were detected mainly in liver and gonads of fish of both sexes. In situ hybridization of esrs along with germ cell (vasa) and gonadal somatic cell markers (gonadal soma-derived factor (gsdf), 3b-hydroxysteroid dehydrogenase (3bhsd), and anti-Mü llerian hormone (amh) for testicular, or gsdf for ovarian somatic cells) showed that all three esrs were preferentially localized within interstitial fibroblasts composed of immature and mature Leydig cells in testis, whereas they were differentially expressed in both follicular cells and oocytes in ovary. Quantitative real-time PCR analysis revealed a sexually dimorphic expression pattern of the three esr paralogs in testis and ovary. A significant increase in esr2a expression was identified in testis and of esr2b in ovary, whereas esr1 transcripts were elevated in both testis and ovary in February and March before the spawning period. The localization and sexually dimorphic expression of esr genes in gonads indicate a direct function of estrogen via ERs in gonadal somatic cell growth and differentiation for Leydig cell in testis and follicular cells in ovary throughout the annual reproductive cycle in Atlantic cod. Key Words" androgen receptor (ar) " Atlantic cod " cytochrome P450 aromatase (cyp19a1a)" estrogen receptor (esr)" sexual maturation

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle

Nagasawa

Presslauer

Kirtiklis

et al. 2014

Journal of Molecular Endocrinology

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“…The preoptic region and lateral tuberal nucleus influence release of reproductive hormones from the anterior pituitary. In teleost fish, several hormones, receptors or enzymes were detected in NPO and NLT by using in situ hybridization and immunological methods, such as GnRH (Baby et al, 2000;Montero et al, 1994;Mousa and Mousa, 2003), Neuropeptide Y (Cerdá-Reverter et al, 2000;Chiba et al, 2002;Peng et al, 1994), FSH and LH (Parhar et al, 2003), melanocyte-stimulating hormone (Chiba, 2001), somatostatin (Cardenas et al, 2000;Mousa and Mousa, 1999), Galanin (Rao et al, 1996;Unniappan et al, 2004), estrogen receptor (Andreassen et al, 2003;Menuet et al, 2002;Pellegrini et al, 2005) and so on. In the present study, the special function of EcMBP is unknown.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides)

Zhou

Wang

et al. 2007

Journal of Chemical Neuroanatomy

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“…ER has been reported to occur in various tissues of fish (Andreassen et al, 2003), lizard (Cardone et al, 1999), and several mammals, including humans (Gustafsson, 1999). A wide distribution of ER␣ immunoreaction has been reported in primate dermal fibroblasts (Bentley et al, 1986), human fibroblasts, endothelial cells (Brandi et al, 1993), mammary gland cells (Pelletier, 2000), chondrocytes (Claassen et al, 2001), human osteoblasts (Eriksen et al, 1988), and osteoclasts (Pensler et al, 1990), in addition to the female reproductive tissues, suggesting diverse effect of estrogen on development, growth, and homeostasis (Clark et al, 1992).…”

mentioning

confidence: 99%

Characteristic distribution of immunoreaction for estrogen receptor alpha in rat ameloblasts

2005

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Estrogen has a diverse function, including cell proliferation and differentiation via estrogen receptors (ER), which have been reported to be the case in various tissues in addition to female reproductive organs. A recent immunocytochemical study has reported the expression of ER␣, a subtype of ER, in rat odontoblasts, suggesting an involvement of estrogen in the differentiation of tooth-forming cells. However, there is no information on the ER␣ immunoexpression in ameloblasts. The present study was therefore undertaken to examine the localization of ER␣ immunoreaction in rat ameloblasts during amelogenesis. A computer-assisted quantitative analysis under a confocal laser scanning microscope was employed to demonstrate the stage-specific localization pattern of ER␣ immunoreaction. Immunohistochemistry of the rat enamel organ revealed ER␣ expression as nuclear localization in ameloblasts, stratum intermedium, stellate reticulum, and papillary layer, in addition to mature and immature odontoblasts. The ratio of immunopositive nuclei to total nuclei (immunopositive ratio) in ameloblasts was high at the apical loop region and gradually declined at the presecretory stage to zero at the secretory stage with statistically significant difference. The ER␣ immunolabeling pattern exhibited a periodic change at the maturation stage proper with constant higher labeling in ruffle-ended ameloblasts than in smooth-ended ameloblasts. The positive ratio was then followed by a statistically significant increase in immunolabeling thereafter. This stage-specific immunolabeling pattern during amelogenesis suggests a possible role of ER␣ in ameloblast proliferation and differentiation.

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Molecular cloning, characterisation, and tissue distribution of oestrogen receptor alpha in eelpout (Zoarces viviparus)

Cited by 46 publications

References 73 publications

Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle

Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle

Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides)

Characteristic distribution of immunoreaction for estrogen receptor alpha in rat ameloblasts

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