Molecular Cloning, Characterization, and Expression Analysis of DROOPING LEAF gene in the Pistillody Line of Common Wheat

Abstract

<p class="NewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNewNew">In this article, we report the cloning, characterization, and expression pattern of <em>Triticum aestivum DROOPING LEAF</em> gene (<em>TaDL</em>) in pistillody line of common wheat. Three <em>TaDL</em> homoeologous genes (<em>TaDL1</em>, <em>TaDL2</em>, and <em>TaDL3</em>) were isolated and found to be located on chromosomes 4AS, 4DL, and 4BL, respectively. The putative <em>TaDL1</em>, <em>TaDL2</em>, and <em>TaDL3</em> protein were 22.4, 22.6, and 22.3 kDa, with theoretical pI of 9.04, 9.04, and 8.98 and containing two distinct domains, namely, a zinc-finger domain in the N-terminal region and a C-terminal YABBY domain. The genome sequence of the three homologous genes consisted seven exons and six introns. All the introns, except for intron VI, varied in length and sequence composition. Phylogenetic analysis revealed that <em>TaDL</em> was most closely related to the <em>Brachypodium distachyon</em> <em>BdDL</em> and <em>Oryza sativa</em> <em>DL</em> genes. The expression pattern analysis indicated that <em>TaDL1</em>, <em>TaDL2</em>,<em> </em>and <em>TaDL3</em> have a higher expression levels in PS and P; however, the three genes are very rare in S. Thus, <em>TaDL</em> gene may contribute to pistil development. Moreover, overexpression of <em>TaDL</em> gene in stamens may cause complete or partial homeosis of stamens into pistils in wheat.</p>

Genome-Wide Identification of the YABBY Gene Family in Seven Species of Magnoliids and Expression Analysis in Litsea

“…The YABBY genes constitute a small transcription factor family characterized by two conserved domains, an N-terminal region C2C2 zinc-finger domain and a C-region YABBY domain [ 7 ]. To examine the structural features of 55 YABBY proteins, multiple sequence alignments analysis of magnoliids YABBY proteins were applied to generate sequence logos of both the C2C2 zinc-finger and YABBY domains in seven magnoliids and Arabidopsis ( Figure 1 ).…”

“…Unlike other transcription factor families, YABBY genes seemed to be absent in non-seed lineages of embryophyte plants [ 5 ]. Additionally, YABBY genes encode putative transcription factors that consist of a zinc-finger domain in the N-terminal and C-terminal YABBY domain, similar to a high-mobility group (HMG) box [ 6 , 7 ]. In Arabidopsis thaliana , the YABBY gene family has a distinct role, declared to six subclades, including CRABS CLAW ( CRC ), INNER NO OUTER ( INO ), FILAMENTOUS FLOWER ( FIL ), YABBY3 ( YAB3 ), YABBY2 ( YAB2 ), and YABBY5 ( YAB5 ) [ 8 , 9 , 10 ].…”

Co-expression network analysis of the genes associated with pistillody-stamen development in wheat

“…Transcription factors play an important role in regulating the development of plants. Some previous studies have reported that MADS-box [9,14,16,17,26,27,28] and DROOPING LEAF gene of YABBY family [6,15,16] associated with the development of wheat pistillody-stamen. We identify MADS-box and YABBY transcription factors from wheat flower substructure tissues (Table 2).…”

“…A total of 4 published YABBY transcription factors are considered to regulate the development of wheat pistiilosy-stamen [6,15,16]. The published 4 proteins of YABBY have been aligned with the 15 180 floral proteins of Additional file 1: Table S1 by BLAST.…”

“…Heterologous expression of the TaEPFL1 gene in Arabidopsis caused shortened filaments and pedicels, which might induce stamens transformation into pistils or pistil-like structures in wheat [13]. Furthermore, some transcription factors of MADS-box and DROOPING LEAF gene of YABBY family have been considered to regulate in the development of wheat pistillody-stamen [6,9,[14][15][16][17][18]. The expression pattern analysis indicate that TaDL1, TaDL2, and…”