Molecular cloning, characterization and expression analysis of WAG-1 in the pistillody line of common wheat

Wang, Q.H.; Yang, Zaijun; Wei, Shuhong; Jiang, Zhongliang; Yang, Yaxuan; Hu, Zhengzheng; Sun, Qianwen; Peng, Zhengsong

doi:10.4238/2015.october.16.12

Cited by 10 publications

(10 citation statements)

References 27 publications

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“…The AG-like gene subfamily includes members involved in the speci cation of stamen, carpel and ovule. Phylogenetic analysis showed that PfAG5 groups into AG-like clade in MADS-box genes of other plants, and is homologous to the A. tauschii AG-type genes as WAG-2f and WAG-2g [27], and Triticum aestivum TaAGL39 [28]. Overexpression of AGL79 in Arabidopsis result in the narrow leaf shape, fewer number of leaves and early owering [29], which is consistent with observed phenotypes of PfAG5 transgenic Arabidopsis plants in the current study ( Fig.…”

Section: Discussionsupporting

confidence: 90%

Overexpression of AGAMOUS-like gene PfAG5 promotes early flowering in Polypogon fugax

Zhang

et al. 2020

Preprint

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Abstract Background: Herbicides are the major tool for controlling large populations of yield depleting weeds. However, overreliance on herbicides has resulted in weed adaptation and herbicide resistance. In recent years, early flowering weed species related to herbicide resistance is emerging, which may cause seed loss before crop harvest, creating a new problem for non-chemical weed management. However, mechanisms regulating early flowering in weedy species is rarely investigated. Results: The MADS-box gene family plays an important role in flowering time regulation and floral organogenesis. In this study, a homolog gene of AGAMOUS sub-family (referred to as PfAG5) of the MADS-box family was cloned from plants of an early flowering Polypogon fugax population resistant to the ACCase inhibitor herbicide (clodinafop-propargyl). The PfAG5 gene was functionally characterized in Arabidopsis thaliana. Over-expression of the PfAG5 gene in Arabidopsis resulted in early flowering with abnormal flowers (e.g. small petals, short plants and reduced seed set) compared to the wild type. The expression of the PfAG5 gene was high in leaves and flowers, but low in pods in transgenic Arabidopsis. The PfAG5 gene was earlier and higher expressed in the resistant (R) than the susceptible (S) P. fugax plants. Furthermore, one protein (FRIGIDA-like protein) interacting with PfAG5 in R P. fugax was identified by the yeast two-hybrid system with relevance to flowering time regulation. Conclusions: These results suggest that the PfAG5 gene is involved in modulating early flowering in P. fugax. This study provides the first evidence for the regulation mechanism of early flowering in an herbicide resistant weed species.

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Section: Discussionsupporting

confidence: 90%

Overexpression of AGAMOUS-like gene PfAG5 promotes early flowering in Polypogon fugax

Zhang

et al. 2020

Preprint

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“…The AG-like gene subfamily includes members involved in the specification of stamen, carpel and ovule. Phylogenetic analysis showed that PfAG5 groups into AG-like clade in MADS-box genes of other plants, and is homologous to the A. tauschii AG-type genes as WAG-2f and WAG-2 g [27], and Triticum aestivum TaAGL39 [28]. Overexpression of AGL79 in Arabidopsis result in the narrow leaf shape, fewer number of leaves and early flowering [29], which are consistent with the phenotypes of PfAG5 transgenic Arabidopsis plants.…”

Section: Discussionsupporting

confidence: 64%

Overexpression of AGAMOUS-like gene PfAG5 promote early flowering in Polypogon fugax

Zhang

Yao

et al. 2020

Preprint

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Background: Herbicides are the major tool for controlling large populations of yield depleting weeds. However, overreliance on herbicides has resulted in weed adaptation and herbicide resistance. In recent years, early flowering weed species related to herbicide resistance is emerging, which may cause seed loss before crop harvest, creating a new problem for non-chemical weed management. However, mechanisms regulating early flowering in weedy species is rarely investigated. Results: The MADS-box gene family plays an important role in flowering time regulation and floral organogenesis. In this study, a homolog gene of AGAMOUS sub-family (referred to as PfAG5) of the MADS-box family was cloned from plants of an early flowering Polypogon fugax population resistant to the ACCase inhibitor herbicide (clodinafop-propargyl). The PfAG5 gene was functionally characterized in Arabidopsis thaliana. Over-expression of the PfAG5 gene in Arabidopsis resulted in early flowering with abnormal flowers (e.g. small petals, short plants and reduced seed set) compared to the wild type. The expression of the PfAG5 gene was high in leaves and flowers, but low in pods in transgenic Arabidopsis. The PfAG5 gene was earlier and higher expressed in the resistant (R) than the susceptible (S) P. fugax plants. Furthermore, one protein (FRIGIDA-like protein) interacting with PfAG5 in R P. fugax was identified by the yeast two-hybrid system with relevance to flowering time regulation. Conclusions: These results suggest that the PfAG5 gene is prominently involved in modulating early flowering in P. fugax. This study provides the first evidence for the regulation mechanism of early flowering in an herbicide resistant weed species.

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“…Transcription factors play an important role in regulating the development of plants. Some previous studies have reported that MADS-box [9,14,16,17,26,27,28] and DROOPING LEAF gene of YABBY family [6,15,16] associated with the development of wheat pistillody-stamen. We identify MADS-box and YABBY transcription factors from wheat flower substructure tissues (Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…Heterologous expression of the TaEPFL1 gene in Arabidopsis caused shortened filaments and pedicels, which might induce stamens transformation into pistils or pistil-like structures in wheat [13]. Furthermore, some transcription factors of MADS-box and DROOPING LEAF gene of YABBY family have been considered to regulate in the development of wheat pistillody-stamen [6,9,[14][15][16][17][18]. The expression pattern analysis indicate that TaDL1, TaDL2, and…”

mentioning

confidence: 99%

Co-expression network analysis of the genes associated with pistillody-stamen development in wheat

Liao¹,

Chen²,

Yang³

et al. 2020

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Background: Crop male sterility has great values in both theoretical research and breeding application. Wheat pistillody-stamen is an important male sterility phenomenon, and HTS-1 is an important pistillody-stamen material. However the molecular mechanism of HTS-1 stamens transformed into pistils or pistil-like structures remains a mystery. Weighted gene co-expression network analysis (WGCNA) are widely used to explore hub genes and gene interaction networks from high throughput data in various plants. Results: In the present study, for exploring gene networks associated with wheat pistillody-stamen development, WGCNA was employed to analyze 11 RNA-sequencing (RNA-seq) data of wheat tissues, including stamens of CSTP, pistils and pistillody-stamen of HTS-1. 19 out of 25 merged modules were highly associated with specific wheat tissues, and the MEdarkseagreen1 module was highly related to wheat pistillody-stamen (correlation with weight r =0.7, correlation p-value p =0.02). Then 180 genes about wheat flower development were identified from the MEdarkseagreen1 module by GO term analysis. Among 180 genes, the hub gene number associated with anther, filament, style, and ovary development were 12, 3, 3, and 10, respectively. We compared the published pistillody related proteins with proteins of HTS-1 by BLAST. A total of 58 pistillody-stamen development associated proteins were validated by BLAST. MADS-box and YABBY transcription factor about pistillody-stamen development were also analyzed in wheat flower. There were 47 of MADS-box and 17 of YABBY transcription factors were identified. BLAST program was used to align the published pistillody associated MADS-box and YABBY transcription factors with transcription factors identified in wheat flower. Totally, 36 of 47 MADS-box and 14 of 17 YABBY transcription factors were considered to regulate the development of pistillody-stamen, which had never been reported yet. Conclusion: These results have systematically identified the key candidate genes about the development of HTS-1 substructures flower. The tissue-specific correlation network analyses provide important insights into the molecular interactions underlying psitillody-stamen development.

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Molecular cloning, characterization and expression analysis of WAG-1 in the pistillody line of common wheat

Cited by 10 publications

References 27 publications

Overexpression of AGAMOUS-like gene PfAG5 promotes early flowering in Polypogon fugax

Overexpression of AGAMOUS-like gene PfAG5 promotes early flowering in Polypogon fugax

Overexpression of AGAMOUS-like gene PfAG5 promote early flowering in Polypogon fugax

Co-expression network analysis of the genes associated with pistillody-stamen development in wheat

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