Molecular cloning, characterization, and expression of Rab5B, Rab6A, and Rab7 from Litopenaeus vannamei (Penaeidae)

Zhao, Yang; Chen, X.L.; Zeng, Dingyuan; Yang, Chunling; Peng, Min; Chen, X.H.

doi:10.4238/2015.july.13.20

Cited by 16 publications

(5 citation statements)

References 21 publications

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“…Since there are many cascades for phagocytosis and various molecules work together, it is difficult to determine which cluster is centralized, but various phagocytosis-related genes are expressed relatively strongly in Hem 5 ( Figure 8—figure supplement 4 ). Rab7 was found to be highly expressed in Hem 1, which is known to be involved in the endosomal trafficking pathway of virus-infected penaeid shrimp ( Zhao et al, 2015 ). However, there have been no detailed functional studies of Rab7 on whether it is involved in phagocytosis in shrimp, so it is early to determine its role in Hem 1.…”

Section: Resultsmentioning

confidence: 99%

Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process

Koiwai

Koyama

Tsuda³

et al. 2021

eLife

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Crustacean aquaculture is expected to be a major source of fishery commodities in the near future. Hemocytes are key players of the immune system in shrimps; however, their classification, maturation, and differentiation are still under debate. To date, only discrete and inconsistent information on the classification of shrimp hemocytes has been reported, showing that the morphological characteristics are not sufficient to resolve their actual roles. Our present study using single-cell RNA sequencing, revealed six types of hemocytes of Marsupenaeus japonicus based on their transcriptional profiles. We identified markers of each subpopulation and predicted the differentiation pathways involved in their maturation. We also predicted cell growth factors that might play crucial roles in hemocyte differentiation. Different immune roles among these subpopulations were suggested from the analysis of differentially expressed immune-related genes. These results provide a unified classification of shrimp hemocytes, which improves the understanding of its immune system.

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Section: Resultsmentioning

confidence: 99%

Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process

Koiwai

Koyama

Tsuda³

et al. 2021

eLife

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“…Another small GTPase involved in endosomal trafficking, Rab7, is apparently vital for WSSV replication 180 . Rab7 also interacts with the VP28 protein and is involved in antiviral immune defence against WSSV and infectious hypodermal and haematopoietic necrosis virus (IHHNV) 181,182 . Although small GTPases are implicated in innate immunity, their role in regulating metabolic reprogramming is largely unknown.…”

Section: Signaling Pathways That Regulate Metabolismmentioning

confidence: 99%

The regulation of shrimp metabolism by the white spot syndrome virus (WSSV)

Kumar

Huang

et al. 2021

Reviews in Aquaculture

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For more than three decades, infectious diseases have been one of the major causes of economic losses in the global shrimp industry. One of the deadliest of the shrimp pathogens is white spot syndrome virus (WSSV), which causes mass mortality in farmed shrimp. Various aspects of WSSV pathogenicity have been studied, but in this review, we will focus only on one of them, namely metabolic reprogramming. WSSV’s modulation of host metabolism affects glycolysis, the tricarboxylic acid (TCA) cycle, glutaminolysis and lipid metabolism. Several signaling pathways are involved in this regulation, including JAK‐STAT, PI3K‐AKT‐mTOR, MAPK and Ras‐Raf‐MEK, with the PI3K‐Akt‐mTOR pathway in particular playing a critical role in activating the WSSV‐induced Warburg effect. Taken together, these changes benefit the virus by facilitating WSSV replication. In this review, we summarize current knowledge regarding the metabolism‐related aspects of WSSV pathogenesis, and discuss the mechanisms by which this virus reprograms its host's metabolic pathways. A deeper understanding of these pathways, key metabolic enzymes and signaling mechanisms is potentially important for the development of effective anti‐WSSV strategies.

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“…In crustaceans, Rab proteins are gradually being studied, which have been proven to participate in antiviral immunity. Rab5B, Rab6A and Rab7 were firstly cloned in Litopenaeus vannamei , and the mRNA expression of LvRab5B, LvRab6A and LvRab7 in the hepatopancreas was significantly up-regulated after infection with infectious hypodermal and hematopoietic necrosis virus or WSSV, implying that LvRab5B, LvRab6A and LvRab7 might be involved in immune response towards viral infection in crustacean [45] . Furthermore, Rab7 has been reported to bind to the WSSV envelope protein VP28 in a dose-dependent manner, and vivo neutralization assay showed that compared with the control group injected with WSSV, the shrimp mortality of the groups injected with WSSV plus the recombinant Pm Rab7 (15%) or WSSV plus anti-Rab7 antibody were both significantly decreased, which suggested that the binding of endogenous Pm Rab7 with VP28 was necessary for WSSV infection in P. monodon , and Pm Rab7 might help WSSV infection by avoiding the degradation of lysosome [46] .…”

Section: Molecular Regulation Of Autophagy In Crustaceansmentioning

confidence: 99%