Molecular cloning, characterization and expression of Lc-Sox11a in large yellow croaker Larimichthys crocea

Jiang, Yonghua; Han, Kunhuang; Chen, Shihai; Hong, Wanshu; Wang, Yilei; Zhang, Ziping

doi:10.1016/j.gene.2015.08.021

Cited by 17 publications

(9 citation statements)

References 41 publications

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“…Unlike most other taxa, sexualization in fish is exceptionally plastic, which may confer an evolutionary advantage in rapidly changing environmental conditions (Todd et al, 2016 ). The sex determining genes in fish appear to vary across species, and several sox forms have been implicated in governing sexual differentiation in diverse fish species (Ito et al, 1995 ; Takamatsu et al, 1995 ; Yokoi et al, 2002 ; Zhou et al, 2002 ; Wang et al, 2003 ; Navarro-Martin et al, 2009 , 2012 ; Zhang et al, 2010 ; Uy et al, 2012 ; Reyhanian Caspillo et al, 2014 ; Jiang et al, 2015 ). Other gene networks regulating sexual differentiation have been well characterized in medaka.…”

Section: Discussionmentioning

confidence: 99%

“…The full length form of sox11a gene ( Lc-sox11a ) in large yellow croaker ( Larimichthys crocea ) also shows temporal—and sex-dependent expression differences in the gonad and brain (Jiang et al, 2015 ). In females, Lc-sox11a expression was greatest in the ovary followed by the brain, eye, and gill.…”

Section: Brain Sexual Differentiation In Example Fish and Reptilian Smentioning

confidence: 99%

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Brain Sexual Differentiation and Requirement of SRY: Why or Why Not?

Rosenfeld

2017

Front. Neurosci.

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Brain sexual differentiation is orchestrated by precise coordination of sex steroid hormones. In some species, programming of select male brain regions is dependent upon aromatization of testosterone to estrogen. In mammals, these hormones surge during the organizational and activational periods that occur during perinatal development and adulthood, respectively. In various fish and reptiles, incubation temperature during a critical embryonic period results in male or female sexual differentiation, but this can be overridden in males by early exposure to estrogenic chemicals. Testes development in mammals requires a Y chromosome and testis determining gene SRY (in humans)/Sry (all other therian mammals), although there are notable exceptions. Two species of spiny rats: Amami spiny rat (Tokudaia osimensis) and Tokunoshima spiny rat (Tokudaia tokunoshimensis) and two species of mole voles (Ellobius lutescens and Ellobius tancrei), lack a Y chromosome/Sry and possess an XO chromosome system in both sexes. Such rodent species, prototherians (monotremes, who also lack Sry), and fish and reptile species that demonstrate temperature sex determination (TSD) seemingly call into question the requirement of Sry for brain sexual differentiation. This review will consider brain regions expressing SRY/Sry in humans and rodents, respectively, and potential roles of SRY/Sry in the brain will be discussed. The evidence from various taxa disputing the requirement of Sry for brain sexual differentiation in mammals (therians and prototherians) and certain fish and reptilian species will be examined. A comparative approach to address this question may elucidate other genes, pathways, and epigenetic modifications stimulating brain sexual differentiation in vertebrate species, including humans.

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Section: Discussionmentioning

confidence: 99%

Section: Brain Sexual Differentiation In Example Fish and Reptilian Smentioning

confidence: 99%

Brain Sexual Differentiation and Requirement of SRY: Why or Why Not?

Rosenfeld

2017

Front. Neurosci.

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“…Sox4 is expressed in the testes and ovaries of M. anguillicaudatus and P. dabryanus, suggesting that sox4 is involved in gonadal development (Xia et al 2017b;Xia et al 2018b). Sox11a is widely distributed in the cytoplasm of germ cells in L. crocea, suggesting that sox11a may be related to in the gametogenesis (Jiang et al 2015). Sox5 also reportedly plays an important role in regulating the number of germ cells of O. latipes (Schartl et al 2018).…”

Section: Gonadal Developmentmentioning

confidence: 98%

A review on sox genes in fish

Wang

2021

Reviews in Aquaculture

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The Sry-related high mobility group box (Sox) genes family is a group of transcription factors playing crucial roles during reproduction and development. At least 50% amino acid sequences of Sox protein family are similarity to the sex-determining region on the Y chromosome (Sry). The sox family exists throughout the invertebrates and vertebrates and plays an important role in sex determination and differentiation, gonadal development, neurogenesis, formation of multiple tissues and organs, early embryonic development, chondrocyte differentiation, cardio-vascular system, and other features in fish. The full characters of sox genes at the genome-wide level have been reported in many fish species, due to the rapid development of high-throughput DNA sequencing technique and massive accumulation of genomic data. Here, the research progress of fish sox gene family is reviewed to provide the latest information for the research in this field.

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“…In the present study in vivo stimulation also modulated pisc expression, with the highest level of expression found in kidney, spleen and gills at 24 h after injection with β-glucan, and in gills after poly (I:C) treatment. In other fish species pisc genes can be induced by a variety of stimuli such as LPS [60,61], parasite antigens, poly (I:C) [62] and β-glucan as a representative PAMP from fungi [58]. In conclusion, the current study is the first identification of AMPs from meagre and demonstrates their expression modulation during development and in response to PAMPs.…”

Section: Discussionmentioning

confidence: 64%

“…was detected in cytoplasmic granules of inflammatory cells, and mast cells [55]. Until recently, piscidins had only been found in fish species from the superorder Acanthopterygii, but there has recently been pisc genes identified in Atlantic cod [56], where expression is seen in more diverse tissues including liver, blood, gall bladder, pyloric caeca, stomach, rectum, muscle, heart, and brain [56][57][58], as well as in mast cells and rodlet cells of the skin, gill and intestine [59].…”

Section: Discussionmentioning

confidence: 99%

Ontogeny and modulation after PAMPs stimulation of β-defensin, hepcidin, and piscidin antimicrobial peptides in meagre ( Argyrosomus regius )

Campoverde

Milne

Estévez

et al. 2017

Fish & Shellfish Immunology

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Antimicrobial peptides (AMPs), components of innate immunity, play an important role in protecting fish. In this study we report the molecular cloning of full open reading frames and characterization of expression of three AMP genes (β-defensin (defb), hepcidin (hep2), piscidin (pisc) in meagre (Argyrosomus regius). A phylogenetic analysis of the expressed sequences obtained shows the defensin isoform forms a clade with the other members of the beta class of this family, hepcidin corresponds to hepcidin 2, and piscidin corresponds to class I of its respective family. Gene expression profiles of AMPs was investigated, by means of quantification of mRNA in nine development stages, from 8 days post-hatching (dph) to accomplishment of juvenile form (120 dph). During development it was demonstrated defb, hep2, pisc were expressed in all stages of larval development and in juvenile tissues (kidney, spleen gut and gill). Moreover, expression patterns suggest the expression levels of theses AMPs are influenced by live prey (rotifer, Artemia) and first intake of commercial diet. Induction experiments in vivo (24 h) and in vitro (4, 12, 24 h) with PAMPs (LPS, poly (I:C), β-glucan) revealed significant changes in gene expression of the three AMP genes, in kidney, spleen, gut and gill. However, expression profiles differed in magnitude and time course response. defb expression shows a similar trend in vivo and in vitro in kidney at 24 h after LPS and β-glucan stimulation. The hep2 expression levels were up-regulated upon β-glucan challenge in vivo, more in gut and gills than kidney, while in vitro hep2 expression was up-regulated in kidney cells by LPS, poly (I:C), β-glucan (4 h). pisc expression was up-regulated in kidney cells, splenocytes by β-glucan, but in gill cells by poly (I:C) and β-glucan in vivo. However, pisc expression was upregulated in kidney cells by β-glucan and gill cells by LPS at 4 post-stimulation in vitro. These data suggest that AMPs play an important role in defense against pathogens, with each AMP having differing efficacies against specific types of microorganisms, although follow-up studies focusing on the biological activities in fish are needed.

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Molecular cloning, characterization and expression of Lc-Sox11a in large yellow croaker Larimichthys crocea

Cited by 17 publications

References 41 publications

Brain Sexual Differentiation and Requirement of SRY: Why or Why Not?

Brain Sexual Differentiation and Requirement of SRY: Why or Why Not?

A review on sox genes in fish

Ontogeny and modulation after PAMPs stimulation of β-defensin, hepcidin, and piscidin antimicrobial peptides in meagre ( Argyrosomus regius )

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