Molecular cloning, expression analysis and cellular localization of an LFRFamide gene in the cuttlefish Sepiella japonica

Cao, Zi-hao; Sun, Lianlian; Chi, Chang-Feng; Liu, Huihui; Zhou, Liqing; Lv, Zhenming; Wu, Changwen

doi:10.1016/j.peptides.2015.10.005

Cited by 10 publications

(10 citation statements)

References 36 publications

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“…Many studies have focused on investigating the physiological characters, environmental adaptations and cultivation improvements in Sepia [8,16,17,18]. However, though several sequencing data records deposited in NCBI database, there’re no systematic dissection on the genome and transcriptome information of Sepia published [19]. The lack of genome information of Sepia has greatly blocked the exploration of genetic background of key physiological features of Sepia and seeking rational solutions on currently obstacles in artificial hatching and scaleful cultivation.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Sequencing and De Novo Assembly of Golden Cuttlefish Sepia esculenta Hoyle

Liu

Zhao

Yan

et al. 2016

IJMS

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Golden cuttlefish Sepia esculenta Hoyle is an economically important cephalopod species. However, artificial hatching is currently challenged by low survival rate of larvae due to abnormal embryonic development. Dissecting the genetic foundation and regulatory mechanisms in embryonic development requires genomic background knowledge. Therefore, we carried out a transcriptome sequencing on Sepia embryos and larvae via mRNA-Seq. 32,597,241 raw reads were filtered and assembled into 98,615 unigenes (N50 length at 911 bp) which were annotated in NR database, GO and KEGG databases respectively. Digital gene expression analysis was carried out on cleavage stage embryos, healthy larvae and malformed larvae. Unigenes functioning in cell proliferation exhibited higher transcriptional levels at cleavage stage while those related to animal disease and organ development showed increased transcription in malformed larvae. Homologs of key genes in regulatory pathways related to early development of animals were identified in Sepia. Most of them exhibit higher transcriptional levels in cleavage stage than larvae, suggesting their potential roles in embryonic development of Sepia. The de novo assembly of Sepia transcriptome is fundamental genetic background for further exploration in Sepia research. Our demonstration on the transcriptional variations of genes in three developmental stages will provide new perspectives in understanding the molecular mechanisms in early embryonic development of cuttlefish.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Sequencing and De Novo Assembly of Golden Cuttlefish Sepia esculenta Hoyle

Liu

Zhao

Yan

et al. 2016

IJMS

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“…At present, more than 40 types of FLPs from the five major mollusk classes (Cephalopoda, Scaphopoda, Bivalvia, Gastropoda, and Polyplacophora) have been isolated and characterized [5,24]. Furthermore, several physiological assays have demonstrated that these FLPs might be involved in food intake [25,26], reproduction [26,27], and heart activity [28]. Moreover, an FMRFamide expressed by a single gene in cephalopods was identified as a member of the FaRP subfamily [28,29].…”

Section: Introductionmentioning

confidence: 99%

An FMRFamide Neuropeptide in Cuttlefish Sepia pharaonis: Identification, Characterization, and Potential Function

Yang

Sun

et al. 2020

Molecules

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Neuropeptides are released by neurons that are involved in a wide range of brain functions, such as food intake, metabolism, reproduction, and learning and memory. A full-length cDNA sequence of an FMRFamide gene isolated from the cuttlefish Sepia pharaonis (designated as SpFMRFamide) was cloned. The predicted precursor protein contains one putative signal peptide and four FMRFamide-related peptides. Multiple amino acid and nucleotide sequence alignments showed that it shares 97% similarity with the precursor FMRFamides of Sepiella japonica and Sepia officinalis and shares 93% and 92% similarity with the SpFMRFamide gene of the two cuttlefish species, respectively. Moreover, the phylogenetic analysis also suggested that SpFMRFamide and FMRFamides from S. japonica and S. officinalis belong to the same sub-branch. Tissue expression analysis confirmed that SpFMRFamide was widely distributed among tissues and predominantly expressed in the brain at the three development stages. The combined effects of SpFMRFamide+SpGnRH and SpFLRFamide+SpGnRH showed a marked decrease in the level of the total proteins released in the CHO-K1 cells. This is the first report of SpFMRFamide in S. pharaonis and the results may contribute to future studies of neuropeptide evolution or may prove useful for the development of aquaculture methods for this cuttlefish species.

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“…RFamides (including FMRFamide) have been implicated in inhibition of feeding in gastropods (Bechtold and Luckman, 2007 ) and a similar role has been proposed for cephalopods (Zatylny-Gaudin et al, 2010 ; Zhang and Tublitz, 2013 ; Cao et al, 2016 ), but no direct evidence has been provided. Peptide GNLRFamide increased tone, contraction frequency and amplitude in the rectum from S. officinalis , but was without effect on either the esophagus or contractile regions of the male or female reproductive tracts (Zatylny-Gaudin et al, 2010 ).…”

Section: Discussionmentioning

confidence: 98%

“…There is considerable evidence for the presence of FMRFamide and/or FMRFamide-related peptides in the central (Di Cosmo and Di Cristo, 1998 ; Suzuki et al, 2002 ; Di Cristo et al, 2003 ; Zatylny-Gaudin et al, 2010 ; Cao et al, 2016 ) and peripheral divisions (e.g., chromatophore motorneurones, Loi et al, 1996 ; stellate ganglion, Burbach et al, 2014 ) of the cephalopod nervous system. The demonstration of pronounced FMRFamide-like IR in octopus gastric ganglion nerve fibers is consistent with other findings reporting the presence of FMRF-amide like peptides in the palliovisceral lobe ( Sepiella japonica , Cao et al, 2016 ), the site of origin of the visceral nerve that connects the brain and gastric ganglion (Young, 1967 , 1971 ), and in rectal nerve endings ( Sepia officinalis , Zatylny-Gaudin et al, 2010 ) that may originate from the gastric ganglion (Young, 1967 ). The FMRFamide and RFamide-like peptides are widely distributed amongst the Mollusca including cephalopods (Walker et al, 2009 ; Zatylny-Gaudin and Favrel, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

The Gastric Ganglion of Octopus vulgaris: Preliminary Characterization of Gene- and Putative Neurochemical-Complexity, and the Effect of Aggregata octopiana Digestive Tract Infection on Gene Expression

et al. 2017

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The gastric ganglion is the largest visceral ganglion in cephalopods. It is connected to the brain and is implicated in regulation of digestive tract functions. Here we have investigated the neurochemical complexity (through in silico gene expression analysis and immunohistochemistry) of the gastric ganglion in Octopus vulgaris and tested whether the expression of a selected number of genes was influenced by the magnitude of digestive tract parasitic infection by Aggregata octopiana. Novel evidence was obtained for putative peptide and non-peptide neurotransmitters in the gastric ganglion: cephalotocin, corticotrophin releasing factor, FMRFamide, gamma amino butyric acid, 5-hydroxytryptamine, molluscan insulin-related peptide 3, peptide PRQFV-amide, and tachykinin–related peptide. Receptors for cholecystokininA and cholecystokininB, and orexin2 were also identified in this context for the first time. We report evidence for acetylcholine, dopamine, noradrenaline, octopamine, small cardioactive peptide related peptide, and receptors for cephalotocin and octopressin, confirming previous publications. The effects of Aggregata observed here extend those previously described by showing effects on the gastric ganglion; in animals with a higher level of infection, genes implicated in inflammation (NFκB, fascin, serpinB10 and the toll-like 3 receptor) increased their relative expression, but TNF-α gene expression was lower as was expression of other genes implicated in oxidative stress (i.e., superoxide dismutase, peroxiredoxin 6, and glutathione peroxidase). Elevated Aggregata levels in the octopuses corresponded to an increase in the expression of the cholecystokininA receptor and the small cardioactive peptide-related peptide. In contrast, we observed decreased relative expression of cephalotocin, dopamine β-hydroxylase, peptide PRQFV-amide, and tachykinin-related peptide genes. A discussion is provided on (i) potential roles of the various molecules in food intake regulation and digestive tract motility control and (ii) the difference in relative gene expression in the gastric ganglion in octopus with relatively high and low parasitic loads and the similarities to changes in the enteric innervation of mammals with digestive tract parasites. Our results provide additional data to the described neurochemical complexity of O. vulgaris gastric ganglion.

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Molecular cloning, expression analysis and cellular localization of an LFRFamide gene in the cuttlefish Sepiella japonica

Cited by 10 publications

References 36 publications

Transcriptome Sequencing and De Novo Assembly of Golden Cuttlefish Sepia esculenta Hoyle

Transcriptome Sequencing and De Novo Assembly of Golden Cuttlefish Sepia esculenta Hoyle

An FMRFamide Neuropeptide in Cuttlefish Sepia pharaonis: Identification, Characterization, and Potential Function

The Gastric Ganglion of Octopus vulgaris: Preliminary Characterization of Gene- and Putative Neurochemical-Complexity, and the Effect of Aggregata octopiana Digestive Tract Infection on Gene Expression

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