1996
DOI: 10.1016/0167-4781(96)00047-4
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Molecular cloning, expression and function of the murine CB2 peripheral cannabinoid receptor

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Cited by 180 publications
(120 citation statements)
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“…Membranes were isolated from CHO cells expressing either hCB1 or hCB2 as described previously Shire et al, 1996). Briefly, cells were washed twice with phosphate-buffered saline (PBS), scraped into 50 mM Tris-HCl, pH 7.7 (buffer A), crushed in a Polytron for 1 min at 7000 rpm/min, then centrifuged for 15 min at 1100g at 4°C.…”
Section: Methodsmentioning
confidence: 99%
“…Membranes were isolated from CHO cells expressing either hCB1 or hCB2 as described previously Shire et al, 1996). Briefly, cells were washed twice with phosphate-buffered saline (PBS), scraped into 50 mM Tris-HCl, pH 7.7 (buffer A), crushed in a Polytron for 1 min at 7000 rpm/min, then centrifuged for 15 min at 1100g at 4°C.…”
Section: Methodsmentioning
confidence: 99%
“…The human CB 2 receptor shares only 44% amino acid sequence identity with the human CB 1 . Later, the mouse (471) and rat (55,186) CB 2 genes were cloned. The mouse CB 2 is 13 amino acids shorter at the COOH terminal and has 82% amino acid sequence identity with the human CB 2 .…”
Section: Structurementioning
confidence: 99%
“…Previous studies have reported a slightly higher conservation between the mRNAs of the rat and human cannabinoid receptors compared with those of the mouse and human (Chakrabarti et al, 1995;Shire et al, 1996). In the rat ileum, the mRNAs of both cannabinoid receptors and their expression have been both identified and mapped in the myenteric plexus Valenti et al, 2005;Duncan et al, 2008).…”
Section: Introductionmentioning
confidence: 97%