Molecular cloning, expression and purification of lactoferrin from Tibetan sheep mammary gland using a yeast expression system

Li, Jianbo; Zhu, Weifei; Luo, Mei-Rong; Honghui, Ren; Tang, Lu; Liao, Honghai; Wang, Yong

doi:10.1016/j.pep.2015.01.008

Cited by 11 publications

(11 citation statements)

References 27 publications

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“…In fact, it places us above of the highest value reported, corresponding to 1200 mg/L [ 29 ]; however, this level of expression was obtained from approximately 3 L of culture medium, three times more than that from the present experiment. Other contributions have reported an rLf expression of 60 mg/L from Tibetan sheep [ 30 ], 12 mg/L from pigs [ 31 ], 40 mg/L from horses [ 32 ], 40 mg/L from yaks [ 33 ], and a smaller level of expression of 2 mg/L from goat lactoferrin [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity

Iglesias-Figueroa

Valdiviezo-Godina

Siqueiros-Cendón

et al. 2016

IJMS

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In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expression of recombinant bovine lactoferrin (rbLf) in Pichia pastoris. After Bach fermentation and purification by molecular exclusion, we obtained an expression yield of 3.5 g/L of rbLf. rbLf and predominantly pepsin-digested rbLf (rbLfcin) demonstrated antibacterial activity against Escherichia coli (E. coli) BL21DE3, Staphylococcus aureus (S. aureus) FRI137, and, in a smaller percentage, Pseudomonas aeruginosa (Ps. Aeruginosa) ATCC 27833. The successful expression and characterization of functional rbLf expressed in Pichia pastoris opens a prospect for the development of natural antimicrobial agents produced recombinantly.

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Section: Discussionmentioning

confidence: 99%

High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity

Iglesias-Figueroa

Valdiviezo-Godina

Siqueiros-Cendón

et al. 2016

IJMS

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“…Tibetan sheep (Ovis aries) are an important livestock resource, raised at an altitude above 3000 m on the QTP [3]. At present, there are more than fifty million Tibetan sheep well adapted to the harsh environment and that provide meat and other economic production for about five million native Tibetan herders [4][5][6]. Based on traditional livestock management, Tibetan sheep graze on the natural grazing lands all year-round [7].…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Protein Levels on Growth Performance, Carcass Traits, Serum Metabolites, and Meat Composition of Tibetan Sheep during the Cold Season on the Qinghai-Tibetan Plateau

Wang

Geng

et al. 2020

Animals

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Dietary protein is a critical nutrient that directly influences the health and production of livestock. Recent studies showed that protein supplements could enhance the growth performance of Tibetan sheep. However, there is a lack of information regarding the influence of dietary protein levels on carcass traits and meat composition. This study investigated the effects of dietary protein levels on growth performance, carcass traits, serum metabolites, and meat composition in Tibetan sheep during the cold season on the Qinghai-Tibetan Plateau. A total of eighteen 1-year-old, healthy, castrated Tibetan sheep with similar initial body weights (31.7 ± 0.72 kg) were randomly divided into three treatment groups with different dietary protein level (low protein (LP, 10.1%); medium protein (MP, 12.1%); high protein (HP, 14.1%)) diets. The results indicated that the Tibetan sheep fed with the MP and HP diets had greater final body weights (BWs), average daily gains (ADGs), and average daily feed intakes (ADFIs) (p < 0.05). The MP and HP diets also improved the hot carcass weight, net meat (including the fat) weight, and bone weight of the sheep significantly (p < 0.05). Besides, the dietary protein levels could significantly affect the serum concentrations of growth hormone (p < 0.05). The diameter of muscle fibers in the MP group was significantly greater than that in the LP group (p < 0.05), while the density of muscle fibers showed the opposite trend. The dietary protein levels only significantly (p < 0.05) influenced the ether extract content and profile content of the longissimus dorsi muscle but had no effect on other parameters of meat composition. In summary, our results indicate that dietary protein levels affect growth performance, carcass traits, and meat composition and that diets containing 12.1% and 14.1% protein are recommended to obtain better production performance and meat products in Tibetan sheep, rather than a diet containing 10.1% protein, during the cold season on the Qinghai-Tibetan Plateau.

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“…Tibetan sheep, an indigenous breed raised at an altitude above 3000 m on the Qinghai‐Tibetan plateau (Zhou et al ), provide meat and income for nomadic and semi‐nomadic people in the region (Dong et al ; Li et al ). Today, Tibetan sheep number approximately 50 million animals, the largest number of livestock in the area, and are an important part of China's sheep industry.…”

Section: Introductionmentioning

confidence: 99%

Effect of supplements during the cold season on the reproductive system in prepubertal Tibetan sheep ewes

Jing

Peng

et al. 2017

Animal Science Journal

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We examined the development of the reproductive system in prepubertal Tibetan sheep ewes when fed only oat hay (CON) or supplemented with either lick blocks (BS) or concentrate feed (CS) during the cold season. The average daily gain of the CS ewes was greater than that of the BS ewes (P < 0.05), which was greater than that of the CON ewes. The same pattern was observed in the number of ovarian follicles (P < 0.001), that is, CS > BS > CON. Serum concentrations of gonadotropin-releasing hormone, follicle-stimulating hormone, luteotrophic hormone, estradiol and progesterone in the CS and BS groups were higher than in the CON group (P < 0.05). The messenger RNA (mRNA) expression of KiSS-1, GPR54 (G protein-coupled receptor 54), ERα (estradiol receptor α) in the hypothalamic anteroventral periventricular area of the CS group were higher than in both the BS and CON groups (P < 0.05), while the BS group was higher than in the CON group (P < 0.05). Similar differences among groups were observed for gonadotropin-releasing hormone receptor mRNA expression in the pituitary, follicle-stimulating hormone receptor and luteinizing hormone receptor mRNA expression in the ovary. These results indicated that the KiSS1/GPR54 system was more active with nutrition or trace mineral supplementation during the cold season. The system stimulated the hypothalamic-pituitary-gonadal axis and enhanced folliclar development in prepubertal Tibetan sheep ewes. We concluded that energy, protein and trace minerals supplements could improve the reproductive performance of Tibetan sheep on the Qinghai-Tibetan plateau.

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Molecular cloning, expression and purification of lactoferrin from Tibetan sheep mammary gland using a yeast expression system

Cited by 11 publications

References 27 publications

High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity

High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity

Effects of Dietary Protein Levels on Growth Performance, Carcass Traits, Serum Metabolites, and Meat Composition of Tibetan Sheep during the Cold Season on the Qinghai-Tibetan Plateau

Effect of supplements during the cold season on the reproductive system in prepubertal Tibetan sheep ewes

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