Molecular cloning, expression of a galectin gene in Pacific white shrimp Litopenaeus vannamei and the antibacterial activity of its recombinant protein

Cha, Gui-Hong; Liu, Yuan; Peng, Ting; Huang, Mingzhu; Xie, Chenying; Xiao, Yu-Chao; Wang, Weina

doi:10.1016/j.molimm.2015.06.014

Cited by 33 publications

(9 citation statements)

References 36 publications

(51 reference statements)

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“…Several studies have examined the differential proteome profile in fish after immune relevant challenges such as infection, stress, and probiotic exposure [ 31 , 32 , 33 ]. Furthermore, FSM contains well-established antimicrobial proteins such as lysozyme, a different type of lectin, several types of heat shock proteins, apolipoprotein 1, and complement [ 34 , 35 , 36 , 37 ]. We demonstrated in this study that CHIT1 protein is also present in FSM of Dasyatis pastinaca and it showed a high enzymatic activity.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Anti-Proliferative Effects of Skin Mucus Derived from Dasyatis pastinaca (Linnaeus, 1758)

et al. 2017

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Resistance to chemotherapy occurs in various diseases (i.e., cancer and infection), and for this reason, both are very difficult to treat. Therefore, novel antimicrobial and chemotherapic drugs are needed for effective antibiotic therapy. The aim of the present study was to assess the antimicrobial and anti-proliferative effects of skin mucus derived from Dasyatis pastinaca (Linnaeus, 1758). Our results showed that skin mucus exhibited a significant and specific antibacterial activity against Gram-negative bacteria but not against Gram-positive bacteria. Furthermore, we also observed a significant antifungal activity against some strains of Candida spp. Concerning anti-proliferative activity, we showed that fish mucus was specifically toxic for acute leukemia cells (HL60) with an inhibition of proliferation in a dose dependent manner (about 52% at 1000 μg/mL of fish skin mucous, FSM). Moreover, we did not observe effects in healthy cells, in neuroblastoma cells (SH-SY5Y), and multiple myeloma cell lines (MM1, U266). Finally, it exhibited strong expression and activity of chitinase which may be responsible, at least in part, for the aforementioned results.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial and Anti-Proliferative Effects of Skin Mucus Derived from Dasyatis pastinaca (Linnaeus, 1758)

et al. 2017

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“…MBL were found in Atlantic cod mucus. 8 Galectins are β-galactoside binding proteins able to bind to 26 and kill 27,28 bacteria (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Immune relevant molecules identified in the skin mucus of fish using -omics technologies

Brinchmann

2016

Mol. BioSyst.

118

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“…The lectin from freshwater crab P. jacquemontii was observed to have opsonic activity that leads to effective clearance of nonself-molecules (Denis et al, 2015). Works carried out by Tasumi and Vasta (2007) showed the role of haemocyte associated lectin from Litopenaeus vennaamei displayed broad antimicrobial activity towards Gram-positive and Gram-negative bacteria and was also observed to act as an opsonin (Cha et al, 2015). It could be presumed that probable presence of hydrophobic amino acids in PrLec could play a role in disturbing the bacterial membrane integrity.…”

Section: Discussionmentioning

confidence: 99%

N‐acetyl‐D ‐glucosamine‐binding lectin ( PrLec ) from the serum of healthy blue swimming crab Portunus reticulatus agglutinates Gram‐positive ( Micrococcus luteus ) and Gram‐negative ( Escherichia coli ) bacteria

Paulchamy

Bhuvaragavan

Sruthi

et al. 2022

Aquaculture Research

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Crabs are economically important crustaceans as they are essential delicacies consumed worldwide and are also an excellent environmental indicator. Portunus reticulatus, the blue swimming crab, is a highly valued seafood throughout Asia. The invertebrates protect themselves from invading pathogens by the innate immune response, and lectins play an essential role in recognizing non–self‐agents. The present study was aimed at purification and bacterial agglutinating properties of lectin from the serum of P. reticulatus. Initially, the species identity was confirmed using the mitochondrial cytochrome oxidase I (mtCOI) region and accessions were submitted to the NCBI and BOLD. Then, the lectin (PrLec) was purified using a chitosan‐based affinity column chosen based on the preliminary characterization consisting of haemagglutination (HA), cross‐adsorption, carbohydrate‐binding specificity and physicochemical parameters. The PrLec showed the highest HA titre value of 64 against Human A and buffalo erythrocytes and greater affinity towards N‐acetyl‐D‐glucosamine. The divalent cations and EDTA did not alter the PrLec activity. The molecular weight of the PrLec was 126 kDa (heterodimers of 66 and 60 kDa). Bacterial agglutination activity of the PrLec was examined with Gram‐negative bacteria, Escherichia coli and Gram‐positive bacteria, Micrococcus luteus. The lectin agglutinated both the bacterial strains efficiently.

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Molecular cloning, expression of a galectin gene in Pacific white shrimp Litopenaeus vannamei and the antibacterial activity of its recombinant protein

Cited by 33 publications

References 36 publications

Antimicrobial and Anti-Proliferative Effects of Skin Mucus Derived from Dasyatis pastinaca (Linnaeus, 1758)

Antimicrobial and Anti-Proliferative Effects of Skin Mucus Derived from Dasyatis pastinaca (Linnaeus, 1758)

Immune relevant molecules identified in the skin mucus of fish using -omics technologies

N‐acetyl‐D ‐glucosamine‐binding lectin ( PrLec ) from the serum of healthy blue swimming crab Portunus reticulatus agglutinates Gram‐positive ( Micrococcus luteus ) and Gram‐negative ( Escherichia coli ) bacteria

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