Molecular Cloning, Expression of minD Gene from Lactobacillus acidophilus VTCC-B-871 and Analyses to Identify Lactobacillus rhamnosus PN04 from Vietnam Hottuynia cordata Thunb.

Abstract

The minD gene encoding an inhibitor cell division MinD homolog from Lactobacillus acidophilus VTCC-B-871 was cloned. We showed that there were 97 % homology between minD genes of L. acidophilus VTCC-B-871 and Lactobacillus rhamnosus GG and Lactobacillus rhamnosus Lc705. Based on the analysis of the DNA sequence data from the L. rhamnosus genome project and sequenced minD gene of L. acidophilus VTCC-B-871, a pair of primers was designed to identified the different minD genes from L. acidophilus ATCC 4356, L. rhamnosus ATCC 11443. Besides, the polymerase chain reaction product of minD gene was also obtained in L. rhamnosus PN04, a strain was isolated from Vietnamese Hottuynia cordata Thunb. In addition, we performed a phylogenetic analysis of the deduced amino acid sequence of MinD homologs from L. acidophilus VTCC-B-871 with the other strains and compared the predicted three-dimension structure of L. acidophilus VTCC-B-871 MinD with Escherichia coli MinD, there are similarity that showed evolution of these strains. The overexpression of L. acidophilus VTCC-B-871 MinD in E. coli led to cell filamentation in IPTG and morphology changes in different sugar stresses, interestingly. The present study is the first report characterizing the Lactobacilus MinD homolog that will be useful in probiotic field.

Comparative Genomics Reveals Biomarkers to Identify Lactobacillus Species

“…Molecular identification using minD gene, which encodes for an inhibitor cell division was carried out for Lactobacillus rhamnosus and L. acidophilus. The gene minD was observed to have high homology to that present in Escherichia coli, implying common evolution [37]. Horizontal gene transfer in Lactobacillus has been reported to be responsible for adapting to changes in lifestyles [38,39].…”

Nanosized Minicells Generated by Lactic Acid Bacteria for Drug Delivery

“…MacDiarmid introduced minicells generated by Shigella flexneri, Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli, and Listeria monocytogenes with diameter about 400 nm [9]. In addition, Lactobacillus rhamnosus and Lactobacillus acidophilus could generate minicells with nanosize as well [111][112][113]. During the cell division cycle, the cells must identify the mid-cell site prepared for cytokinesis and division septum ( Figure 3).…”

“…L. rhamnosus and L. acidophilus in combination with glucose and fructose occurred to produce the highest minicells with the size less than 400 nm that could be used as nanocells in delivering cisplatin, paclitaxel, and cephalosporin [111][112][113].…”

Non-traditional sources for isolation of lactic acid bacteria

“…LAB are designated "generally recognized as safe" (GRAS status), and have long played an important role in food technology-they are responsible for lactic fermentation in several products, including dairy, meat, and silage. LAB can be isolated from traditional sources such as raw milk, dairy products and fermented foods (Wouters et al 2002 ;Kimoto et al 2004;Tamang et al 2005;Nomura et al 2006;Kostinek et al 2007;Tanasupawat et al 2007;Kivanc et al 2011;Venturi et al 2012;Abegaz 2014), and from alternative sources including fecal samples, soil and plants (Hartnett et al 2002;Magnusson et al 2003;Cock and de Stouvenel 2006;Siezen et al 2008;Trias et al 2008;Di Cagno et al 2009;Cakir 2010;Chen et al 2010;Venugopalan et al 2010; Baradaran et al 2012;Emerenini et al 2013;Fhoula et al 2013;Nguyen et al 2013;Alemayehu et al 2014). In recent years, there has been increasing interest in LAB isolated from non-dairy sources due to their diverse metabolic profile and unique flavor-forming activities.…”

“…St John's wort has traditionally been used in the form of homeopathic medicines as an alternative treatment for various depressive disorders of mild to moderate severity (Barnes et al 2001). The incorporation of plant extracts in preparations for topical wound treatment has also been successful (Newall et al 1996). Husain et al (2011) demonstrated significant hypolipidemic activity for the Hypericum perforatum L. extract in a rat model.…”