Molecular cloning, expression profiling and RNA interference of a vitellogenin gene from Harmonia axyridis (Coleoptera: Coccinellidae)

Liang, Chao; Liu, Tinghui; Han, Shipeng; He, Yunzhuan

doi:10.1016/s2095-3119(18)62103-3

Cited by 6 publications

(6 citation statements)

References 31 publications

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“…We found that the expression of four Vg ‐related genes ( BdVg1 , BdVg2 , BdVg3 and BdVgR ) was down‐regulated in Bdpnr +/− mutants, coincident with reductions in both the number of eggs laid and the egg hatching rate. Similar results have been reported for RNAi experiments in other insects, including the knockdown of AaGATAa in A. aegypti 19 and BmGATAβ4 in B. mori , the latter also reducing the egg size and egg tube length 37 . In Nilaparvata lugens , knockdown of the NlGATA‐1 gene reduced the basal level of NlVg expression and resulted in the production of fewer offspring 38 .…”

Section: Discussionsupporting

confidence: 81%

“…Vg ‐related genes directly affect ovarian development in insects 6 . For example, the silencing of ZcVg genes in Z. cucurbitae significantly delayed ovarian development, 41 and the knockdown of HmVg2 in Harmonia axyridis decreased the number of eggs laid and the egg hatching rate 37 . In Corcyra cephalonica , the ovaries of females injected with CceVg double‐stranded RNA (dsRNA) were severely deformed, with short ovarioles, unordered egg sizes and few fully developed eggs 42 .…”

Section: Discussionmentioning

confidence: 99%

“…Similar results have been reported for RNAi experiments in other insects, including the knockdown of AaGATAa in A. aegypti 19 and BmGATA⊎4 in B. mori, the latter also reducing the egg size and egg tube length. 37 In Nilaparvata lugens, knockdown of the NlGATA-1 gene reduced the basal level of NlVg expression and resulted in the production of fewer offspring. 38 Consistent with these studies, we found that Bdpnr directly regulates BdVg1-3 in vivo.…”

Section: Discussionmentioning

confidence: 99%

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Pannier is a key regulator of embryogenesis, pupal development and female reproduction in the insect pest Bactrocera dorsalis

Zhang

Dou

et al. 2022

Pest Management Science

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Background: Most arthropods are famous for their large reproductive capacity, with the ovary playing a vital role in the process.The study of the regulatory mechanisms of ovarian development may have the potential for a reproduction-based pest management strategy. GATA-binding transcription factors (GATAs) as important regulatory factors mediate many physiological processes, including development, immunity, insecticide resistance and reproduction. The Pannier (pnr), a member of GATA family, was confirmed to be involved in ovarian development of Bactrocera dorsalis in our previous study. However, the direct evidence of pnr regulating the fly ovarian development is still lacking.Results: We used CRISPR/Cas9 to create Bdpnr loss-of-function mutations. Homozygous Bdpnr −/− mutants were nonviable, with most individuals dying during embryogenesis, some surviving to the larval stages, and the remaining few dying during pupation. In contrast, heterozygous individuals reached the adult stage, but ovarian development was disrupted, with concomitant decreases in egg laying and hatching rates. We also found that two genes encoding vitellogenin proteins (BdVg1 and BdVg2) and the vitellogenin receptor (BdVgR) were significantly down-regulated in heterozygous mutants compared to wild-type controls.Conclusion: These results indicate that Bdpnr is required for embryonic and post-embryonic development, including the formation of ovaries. Bdpnr could therefore be considered as a molecular target for tephritid fly pest control.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Pannier is a key regulator of embryogenesis, pupal development and female reproduction in the insect pest Bactrocera dorsalis

Zhang

Dou

et al. 2022

Pest Management Science

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“…However, the number of these cleavage sites was different in various insects. For example, the HmVg of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) contains three putative cleavage recognition sites [ 31 ], while the amino acid sequence of CsVg in Chrysopa septempunctata (Wesmael) (Neuroptera: Chrysopidae) contains only one RXXR cleavage site [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Vitellogenin and Its Receptor in Spodoptera frugiperda (J. E. Smith, 1797), and Their Function in Reproduction of Female

Han

Wang

Song

et al. 2022

IJMS

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The fall armyworm Spodoptera frugiperda is a highly polyphagous invasive pest. The strong reproductive capacity is an important factor in the rapid colonization and expansion of S. frugiperda. Vitellogenin (Vg) and vitellogenin receptor (VgR) play important roles in insect reproduction. As the precursor of vitellin (Vn), Vg provides essential nutrition for embryonic development, and VgR mediates the uptake of Vg by oocytes. In this context, we cloned and characterized these two genes of S. frugiperda (SfVg and SfVgR) and evaluated their expression profiles in different developmental stages and tissues. The RNA interference experiment was used to investigate their function in vitellogenesis. The ORF values of SfVg and SfVgR were 5250 and 5445 bp, encoding 1749 and 1815 amino acid residues, respectively. The qRT-PCR results revealed that both SfVg and SfVgR were highly expressed in female adults; SfVg was specifically expressed in the fat body, whereas SfVgR was highly expressed in the ovary. In addition, the depletion of either SfVg or SfVgR hindered oocyte maturation and ovarian development, leading to a significant decrease in fecundity. The present study reveals the importance of SfVg and SfVgR in the vitellogenesis of S. frugiperda, laying a theoretical foundation for the development of pollution-free pest control strategies with SfVg and SfVgR as new targets.

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“…Different studies have shown that silencing of the Vg gene in insects can reduce the fitness of the offspring of a treated female. This effect is known as a parental-RNAi effect, a desirable feature for biotechnological products focused on insect pest control [ 48 , 49 , 50 , 51 , 52 ]. In addition, genes related to hormonal regulation are generally effective in triggering lethal phenotypes in RNAi experiments.…”

Section: Introductionmentioning

confidence: 99%

Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (Anthonomus grandis)

Ribeiro

Vasquez

Macedo

et al. 2022

IJMS

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Cotton is the most important crop for fiber production worldwide. However, the cotton boll weevil (CBW) is an insect pest that causes significant economic losses in infested areas. Current control methods are costly, inefficient, and environmentally hazardous. Herein, we generated transgenic cotton lines expressing double-stranded RNA (dsRNA) molecules to trigger RNA interference-mediated gene silencing in CBW. Thus, we targeted three essential genes coding for chitin synthase 2, vitellogenin, and ecdysis-triggering hormone receptor. The stability of expressed dsRNAs was improved by designing a structured RNA based on a viroid genome architecture. We transformed cotton embryos by inserting a promoter-driven expression cassette that overexpressed the dsRNA into flower buds. The transgenic cotton plants were characterized, and positive PCR transformed events were detected with an average heritability of 80%. Expression of dsRNAs was confirmed in floral buds by RT-qPCR, and the T1 cotton plant generation was challenged with fertilized CBW females. After 30 days, data showed high mortality (around 70%) in oviposited yolks. In adult insects fed on transgenic lines, chitin synthase II and vitellogenin showed reduced expression in larvae and adults, respectively. Developmental delays and abnormalities were also observed in these individuals. Our data remark on the potential of transgenic cotton based on a viroid-structured dsRNA to control CBW.

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Molecular cloning, expression profiling and RNA interference of a vitellogenin gene from Harmonia axyridis (Coleoptera: Coccinellidae)

Cited by 6 publications

References 31 publications

Pannier is a key regulator of embryogenesis, pupal development and female reproduction in the insect pest Bactrocera dorsalis

Pannier is a key regulator of embryogenesis, pupal development and female reproduction in the insect pest Bactrocera dorsalis

Molecular Characterization of Vitellogenin and Its Receptor in Spodoptera frugiperda (J. E. Smith, 1797), and Their Function in Reproduction of Female

Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (Anthonomus grandis)

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