Molecular cloning, function prediction and phylogenetic analysis of LMW glutenin subunit genes in <i>Triticum timopheevii</i> (Zhuk.)

Zhang, M. Y.; Wang, K.; Wang, S. L.; Li, X. H.; Zeller, F. J.; Hsam, S. L. K.; Yan, Yueming

doi:10.1111/j.1439-0523.2010.01768.x

Cited by 4 publications

(5 citation statements)

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“…A more detailed study is required to characterize the four novel alleles. Recently, numerous LMW-GS genes have been cloned and characterized ( Jiang et al 2008 , Li et al 2008 , Zhang et al 2010 , Zhao et al 2006 , 2008 ). Based on the available knowledge, the newly identified Glu-3 alleles should be readily cloned and analyzed.…”

Section: Discussionmentioning

confidence: 99%

Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS

Peng

Islam

et al. 2016

Breed. Sci.

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Low molecular weight glutenin subunits are important components of wheat storage proteins, which play an important role in determining end-use quality of common wheat. A newly established matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) procedure was used to analyze 478 landraces of bread wheat collected from the Yangtze-River region in China. Results indicated that 17 alleles at three loci: Glu-A3, Glu-B3 and Glu-D3 were identified, resulting in 87 different allele combinations. Of the 17 alleles detected at all the Glu-3 loci, five belonged to Glu-A3, seven to Glu-B3 and five to Glu-D3 locus. MALDI-TOF-MS indicated Glu-A3a/c was present in 72.8%, Glu-A3b in 8.4%, Glu-A3d in 8.4%, Glu-A3f in 5.2% and Glu-A3e in 3.6% lines. Seven types of alleles were identified at the Glu-B3 locus: Glu-B3d/i (25.5%), Glu-B3b (21.3%), Glu-B3c (16.9%), Glu-B3h (13.8%), Glu-B3f (8.4%), Glu-B3a (8.2%), and Glu-B3g (5.2%). Five types of Glu-D3 alleles were detected: Glu-D3a (58.4%), Glu-D3c (22.6%), Glu-D3d (15.5%), Glu-D3b (3.3%) and Glu-D3f (0.2%). Four new alleles that showed abnormal MALDI-TOF spectrum patterns were identified at the Glu-A3 and Glu-B3 loci. A detailed study is needed to further characterize these alleles and their potential usage for wheat improvement.

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Section: Discussionmentioning

confidence: 99%

Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS

Peng

Islam

et al. 2016

Breed. Sci.

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“…All accessions were kindly provided by the International Maize and Wheat Improvement Center, Mexico. A total of 20 cloned LMW-GS genes, 16 genes separated previously by our group Pei et al 2007;Jiang et al 2008;Zhang et al 2010), and 43 genes from the GenBank database (http://www.ncbi.nlm.nih.gov/Genbank) were selected to construct phylogenetic trees. The LMW-GS genes from Aegilops and Triticum species were encoded by seven genomes, namely A, D, S (S s , S l ), C, U, N, and M. In addition, nine storage protein genes from eight related cereal species involving E e , I, K, R, S t , and V were used as the out group for investigating phylogenetic relationships among Aegilops and Triticum genomes.…”

Section: Plant Materialsmentioning

confidence: 99%

“…In addition to the importance for dough quality, the extensive allelic variations and specific structural features of LMW-GS genes could provide useful information for studies on the origin and evolution of wheat and related species. Recently, we have reported the existence of Glu-3 loci in some related genomes such as G, M, S, and U (Li et al 2008aJiang et al 2008;Zhang et al 2010). In the present study, 70 LMW-GS genes from Triticum and Aegilops species, as well as nine storage protein genes from related cereal species other than the above mentioned, were used to construct phylogenetic trees.…”

Section: Introductionmentioning

confidence: 97%

Phylogenetic analysis of C, M, N, and U genomes and their relationships with Triticum and other related genomes as revealed by LMW-GS genes at Glu-3 loci

Wang

et al. 2011

Genome

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Phylogenetic relationships between the C, U, N, and M genomes of Aegilops species and the genomes of common wheat and other related species were investigated by using three types of low-molecular-weight glutenin subunit (LMW-GS) genes at Glu-3 loci. A total of 20 LMW-GS genes from Aegilops and Triticum species were isolated, including 11 LMW-m type and 9 LMW-i type genes. Particularly, four LMW-m type and three LMW-i type subunits encoded by the genes on the C, N, and U genomes possessed an extra cysteine residue at conserved positions, which could provide useful information for understanding phylogenetic relationships among Aegilops and Triticum genomes. Phylogenetic trees constructed by using either LMW-i or the combination of LMW-m and LMW-s, as well as analysis of all the three types of LMW-GS genes together, demonstrated that the C and U genomes were closely related to the A genome, whereas the N and M genomes were closely related to the D genome. Our results support previous findings that the A genome was derived from Triticum uratu, the B genome was from Aegilops speltoides, and the D genome was from Aegilops tauschii. In addition, phylogenetic relationships among different genomes analysed in this study support the concept that Aegilops is not monophyletic.

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“…Zhang et al [15] and Li et al [16] studied the HMW glutenin subunits, whereas Zhang et al [17] and Wang et al [18] focused on LMW glutenin subunits. From this it appears that, in case of multigene families, it may be necessary to infer relationships at the level of groups of closely related genes rather than for individual genes.…”

Section: Introductionmentioning

confidence: 99%

Expansion of the gamma-gliadin gene family in Aegilops and Triticum

et al. 2012

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BackgroundThe gamma-gliadins are considered to be the oldest of the gliadin family of storage proteins in Aegilops/Triticum. However, the expansion of this multigene family has not been studied in an evolutionary perspective.ResultsWe have cloned 59 gamma-gliadin genes from Aegilops and Triticum species (Aegilops caudata L., Aegilops comosa Sm. in Sibth. & Sm., Aegilops mutica Boiss., Aegilops speltoides Tausch, Aegilops tauschii Coss., Aegilops umbellulata Zhuk., Aegilops uniaristata Vis., and Triticum monococcum L.) representing eight different genomes: Am, B/S, C, D, M, N, T and U. Overall, 15% of the sequences contained internal stop codons resulting in pseudogenes, but this percentage was variable among genomes, up to over 50% in Ae. umbellulata. The most common length of the deduced protein, including the signal peptide, was 302 amino acids, but the length varied from 215 to 362 amino acids, both obtained from Ae. speltoides. Most genes encoded proteins with eight cysteines. However, all Aegilops species had genes that encoded a gamma-gliadin protein of 302 amino acids with an additional cysteine. These conserved nine-cysteine gamma-gliadins may perform a specific function, possibly as chain terminators in gluten network formation in protein bodies during endosperm development. A phylogenetic analysis of gamma-gliadins derived from Aegilops and Triticum species and the related genera Lophopyrum, Crithopsis, and Dasypyrum showed six groups of genes. Most Aegilops species contained gamma-gliadin genes from several of these groups, which also included sequences from the genera Lophopyrum, Crithopsis, and Dasypyrum. Hordein and secalin sequences formed separate groups.ConclusionsWe present a model for the evolution of the gamma-gliadins from which we deduce that the most recent common ancestor (MRCA) of Aegilops/Triticum-Dasypyrum-Lophopyrum-Crithopsis already had four groups of gamma-gliadin sequences, presumably the result of two rounds of duplication of the locus.

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Molecular cloning, function prediction and phylogenetic analysis of LMW glutenin subunit genes in Triticum timopheevii (Zhuk.)

Cited by 4 publications

References 45 publications

Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS

Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS

Phylogenetic analysis of C, M, N, and U genomes and their relationships with Triticum and other related genomes as revealed by LMW-GS genes at Glu-3 loci

Expansion of the gamma-gliadin gene family in Aegilops and Triticum

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