Molecular cloning, genomic structure and interactions of the putative breast tumor suppressor TACC2

Lauffart, Brenda; Gangisetty, Omkaram; Still, Ivan H.

doi:10.1016/s0888-7543(02)00039-3

Cited by 41 publications

(60 citation statements)

References 24 publications

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“…3G). This contrasts to the high levels of expression of the predominant TACC2l isoform noted in the adult heart (Lauffart et al, 2003).…”

Section: The Cardiovascular Systemcontrasting

confidence: 67%

“…6). Expression data on TACC2 and TACC3 are compiled from published data (Still et al, 1999a, b;Sadek et al, 2003;Lauffart et al, 2003;Aitola et al, 2003). (Ϫ, Ϫ/ϩ, ϩϩ, ϩϩϩ, ϩϩϩϩ) represent increasing degrees of expression of the isoform, based upon amplification in the linear range compared to 18S RNA.…”

Section: Differential Expression Of Tacc1 Splice Variants In Adult Humentioning

confidence: 99%

“…Initial analysis has suggested that Tacc1 and Tacc3 mRNA levels are expressed at high levels during embryogenesis and are then downregulated as differentiation proceeds, finally being expressed either at low levels or only in restricted tissues (Still et al, 1999a;Sadek et al, 2000). By contrast, Tacc2 is expressed throughout development and this expression persists in the majority of adult tissues (Lauffart et al, 2003;Schuendeln et al, 2004), although changes in the distribution of splice variants may occur in tissue progenitor cells (Still et al, unpublished data). The importance of these analyses is underscored in the interpretation of studies detailing the targeting of the TACC orthologues in mouse.…”

Section: Introductionmentioning

confidence: 99%

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Temporal and spatial expression of TACC1 in the mouse and human

Lauffart¹,

DiMatteo²,

Vaughan³

et al. 2006

Dev. Dyn.

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TACC1 is the founding member of the evolutionarily conserved transforming acidic coiled coil genes. These genes play a role in normal development and tumorigenesis through interactions with multiple complexes involved in transcription, translation, and centrosomal dynamics. Despite its importance, detailed examination of the expression of TACC1 and splice variants has not previously been performed. In this study, the spatiotemporal distribution of the Tacc1 protein was examined immunohistochemically in cross-sections of mouse embryonic tissues. We also report the distribution of currently known/predicted TACC1 splice variants in adult humans. These results indicate that Tacc1 is regulated in a dynamic manner during embryogenesis. In adult humans, ubiquitous expression of at least one TACC1 splice variant is noted, although specific combinations of variants are evident in individual differentiated tissues. An important observation is that in the in vivo three-dimensional tissue architecture of the growing organism, both the human and mouse TACC1 protein can be localized to different subcellular compartments in a cell-and tissue-specific manner. This indicates that exploration of TACC1 function must take into account the temporal expression of specific splice variants that may perform different cell-type and tissue-specific functions. Furthermore, this analysis will provide the groundwork from which future Tacc1 knockout strategies can be designed and properly interpreted.

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“…3G). This contrasts to the high levels of expression of the predominant TACC2l isoform noted in the adult heart (Lauffart et al, 2003).…”

Section: The Cardiovascular Systemcontrasting

confidence: 67%

Section: Differential Expression Of Tacc1 Splice Variants In Adult Humentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Temporal and spatial expression of TACC1 in the mouse and human

Lauffart¹,

DiMatteo²,

Vaughan³

et al. 2006

Dev. Dyn.

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“…Significance of TACC2 in breast cancer is controversial. TACC2 was identified as a putative breast tumor suppressor 11 and overexpression of TACC2 reverted a malignant phenotype to a benign phenotype 13. On the other hand, single‐nucleotide polymorphisms (SNPs) in TACC2 were significantly associated with a risk of low‐grade breast cancer 25, and TACC2 mRNA expression was significantly correlated with shorter disease‐free survival of breast carcinomas patients 12.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, in our study, we examined TACC2 in breast carcinoma by immunohistochemistry and in vitro experiments to investigate its clinical significance and biological functions. Since TACC2 consists of two isoforms (i.e., major 4.2 kb transcript and minor 9.7 kb transcript) and the major isoform is predominantly expressed in the breast 13, we examined the major isoform of TACC2 in the present in vitro studies.…”

Section: Introductionmentioning

confidence: 99%

TACC2 (transforming acidic coiled‐coil protein 2) in breast carcinoma as a potent prognostic predictor associated with cell proliferation

et al. 2016

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Transforming acidic coiled‐coil protein 2 (TACC2) belongs to TACC family proteins and involved in a variety of cellular processes through interactions with some molecules involved in centrosomes/microtubules dynamics. Mounting evidence suggests that TACCs is implicated in the progression of some human malignancies, but significance of TACC2 protein in breast carcinoma is still unknown. Therefore, in this study, we examined the clinical significance of TACC2 in breast carcinoma and biological functions by immunohistochemistry and in vitro experiments. Immunohistochemistry for TACC2 was performed in 154 cases of invasive ductal carcinoma. MCF‐7 and MDA‐MB‐453 breast carcinoma cell lines were transfected with small interfering RNA (siRNA) for TACC2, and subsequently, cell proliferation, 5‐Bromo‐2′‐deoxyuridine (BrdU), and invasion assays were performed. TACC2 immunoreactivity was detected in 78 out of 154 (51%) breast carcinoma tissues, and it was significantly associated with Ki‐67 LI. The immunohistochemical TACC2 status was significantly associated with increased incidence of recurrence and breast cancer‐specific death of the patients, and multivariate analyses demonstrated TACC2 status as an independent prognostic factor for both disease‐free and breast cancer‐specific survival. Subsequent in vitro experiments showed that TACC2 significantly increased the proliferation activity of MCF‐7 and MDA‐MB‐453. These results suggest that TACC2 plays an important role in the cell proliferation of breast carcinoma and therefore immunohistochemical TACC2 status is a candidate of worse prognostic factor in breast cancer cases.

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Expression pattern of an evolutionarily conserved splice variant in the rat Tacc2 gene

Padhi

Zigler

Padhi

et al. 2014

Genesis

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The transforming acidic coiled-coil containing protein 2 (Tacc2) gene and its paralogs, Tacc1 and Tacc3 encode proteins that are associated with the centrosome and involved in microtubule assembly during the cell cycle. Tacc2 produces several splice variants, which are poorly characterized, especially in the rat. Characterization of the temporal/spatial expression patterns of these isoforms would be useful in understanding their distinct and overlapping functions. By comparative sequence analyses of Tacc2 in multiple species, we identified a third splice variant in rat, which is much shorter in size (1,021 aa) than the longest isoform (2,834 aa). This newly identified Tacc2 splice variant (isoform 3) uses a distinct first exon and generates a different open reading frame. Although Isoform 3 is expressed predominantly during developmental stages, the long Tacc2 isoform (isoform 1) is distributed mainly in adult tissues. Multiple protein sequence analyses revealed that Tacc2 Isoform 3 could be the ancient form, as it is conserved in mammals, birds, and amphibians; whereas the long Tacc2 isoforms may have evolved in the mammalian lineage by adding exons toward the 5' region of the ancient isoform.

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Molecular cloning, genomic structure and interactions of the putative breast tumor suppressor TACC2

Cited by 41 publications

References 24 publications

Temporal and spatial expression of TACC1 in the mouse and human

Temporal and spatial expression of TACC1 in the mouse and human

TACC2 (transforming acidic coiled‐coil protein 2) in breast carcinoma as a potent prognostic predictor associated with cell proliferation

Expression pattern of an evolutionarily conserved splice variant in the rat Tacc2 gene

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