Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus

Li, Wei; Sun, Wenxiu; Meng, Liang; Hong, Dengwei

doi:10.2478/s11756-013-0307-y

Cited by 12 publications

(8 citation statements)

References 48 publications

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“…A detailed characterization of MHC II in eel was beyond the scope of this study and thus we concentrated on the tissue specific expression. The mhc2 expression pattern observed in the present analysis is similar to tissue expression recorded in swamp eel where hardly any expression was detected in heart and muscle, whilst the highest expression was observed in the stomach, spleen and skin [79]. Certain genotypes of MHC class II were shown to be associated with higher survival of carp to CyHV-3 [80].…”

Section: Tissue Specific Expression and Anghv-1 Infectionsupporting

confidence: 82%

Molecular ontogeny of larval immunity in European eel at increasing temperatures

Miest

Politis

Adamek³

et al. 2019

Fish & Shellfish Immunology

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Temperature is a major factor that modulates the development and reactivity of the immune system. Only limited knowledge exists regarding the immune system of the catadromous European eel, Anguilla anguilla, especially during the oceanic early life history stages. Thus, a new molecular toolbox was developed, involving tissue specific characterisation of 3 housekeeping genes, 9 genes from the innate and 3 genes from the adaptive immune system of this species. The spatial pattern of immune genes reflected their function, e.g. complement component c3 was mainly produced in liver and il10 in the head kidney. Subsequently, the ontogeny of the immune system was studied in larvae reared from hatch to first-feeding at four temperatures, spanning their thermal tolerance range (16, 18, 20, and 22°C). Expression of some genes (c3 and igm) declined post hatch, whilst expression of most other genes (mhc2, tlr2, il1β, irf3, irf7) increased with larval age. At the optimal temperature, 18°C, this pattern of immune-gene expression revealed an immunocompromised phase between hatch (0 dph) and teeth-development (8 dph). The expression of two of the studied genes (mhc2, lysc) was temperature dependent, leading to increased mRNA levels at 22°C. Additionally, at the lower end of the thermal spectrum (16°C) immune competency appeared reduced, whilst close to the upper thermal limit (22°C) larvae showed signs of thermal stress. Thus, protection against pathogens is probably impaired at temperatures close to the critical thermal maximum (CTmax), impacting survival and productivity in hatcheries and natural recruitment.

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Section: Tissue Specific Expression and Anghv-1 Infectionsupporting

confidence: 82%

Molecular ontogeny of larval immunity in European eel at increasing temperatures

Miest

Politis

Adamek³

et al. 2019

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 93%

“…The sequence analysis confirms the structural conservation between MAMIT and MACTSB and other vertebrates. These results were consistent with other reported genes of M. albus (Li, Sun, et al, 2011; Li, Sun, Meng, & Hong, 2014) and traditional taxonomy of fish (http://fishdb.sinica.edu.tw/AjaxTree/tree.php). Conservation of amino acid sequence and genomic structure of MIFs and CTSBs between M. albus and teleost fish suggests that they might have a similar immunological function.…”

Section: Discussionsupporting

confidence: 92%

The char of migration inhibitory factor and Cathepsin B from Asian swamp eel (Monopterus albus) and their response to challenge with Aeromonas hydrophila

Wang

Lin

et al. 2021

J World Aquaculture Soc

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Aeromonas hydrophila causes sepsis and is responsible for significant economic losses in Asian finless eel (Monopterus albus) aquaculture. Previous studies have found that macrophage migration inhibitory factor (MIF) and Cathepsin B (CTSB) may be expressed in many vertebrate tissues and organs. However, there have been no reports discussing the importance of MIF and CTSB in Asian swamp eel. The complete ORF sequences of MAMIF and MACTSB were found to be 348 and 993 bp, respectively. The adaptive analysis showed that the sequences of MAMIF and MACTSB were highly conserved, and only two positive selection sites were identified in MAMIF. Quantitative real time polymerase chain reaction (qRT‐PCR) demonstrated that MAMIF and MACTSB genes were expressed in all tissues evaluated with the highest expression occurring in the head kidney, spleen and liver. Challenge with Aeromonas hydrophila resulted in a significant increase in the expression of MAMIF and MACTSB in skin, liver, head kidney and spleen that peaked at 12‐h post‐infection. The results show that MAMIF and MACTSB play important roles in the immune function of Monopterus albus.

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“…As it has been mentioned earlier, M. cuchia can tolerate harsh conditions, its culture is easier and highly profitable than a number of other small size fish culture activities [14,15]. Several techniques of capturing the fish have been established, however, no cultural practice has been consecrated and only a few works have from different aspects been done on this so far [6,5,[16][17][18][19][20][21][22]. The mud eel has also been identified for polyculture in seasonal and perennial ponds as well as in paddy fields, and in Bangladesh, some experiments were performed in captivity for observing growth, survival and diet condition [23].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity of Freshwater Mud Eel (Monopterus cuchia) Using RAPD and RFLP Markers

Miah¹,

Anwar²,

Ali³

et al. 2018

J Aquac Res Development

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Background: Monopterus cuchia, an economically important eel of Bangladesh, is confronting the challenge of population reduction and germplasm degeneration since the vast majority of fingerlings are collected from natural habitats and artificial cultivation of the fish is yet to be established. Materials and Methods: 30 individuals of M. cuchia from a natural population of Northern-East part of Bangladesh were analyzed using 8 decamer primers and glutamine synthetase gene was digested with 2 restriction enzymes. Results: A total of 735 bands with 228 polymorphic loci were detected among the selected 30 individuals by using RAPD assay while 100% polymorphism was revealed by all the primers. The genetic distance among the individuals was calculated by using the data from pair-wise similarity index where 36 groups of genetic diversities were measured while the lowest and highest genetic distance were found 0.58 and 0.97 respectively with an average genetic diversity of 0.81. The Nei's genetic similarity values were found from 0.06 to 0.6 where average value was recorded 0.301746 which was found significant regarding genetic distance. Phylogenetic relationships using UPGMA clustering revealed linkage distance ranged from 3.6 to 6.24 and generated 6 clusters by 11 clades with the involvement of 22 individuals while the rest of the samples were connected to those clades with specific linkage distances. Genetic diversity of glutamine synthetase gene was analyzed with two restriction enzymes, e.g. +CfrI and +Hpy178III while both the enzymes digested the gene fragment at a length of 541 bp and polymorphism was detected in terms of wild type homogygotes, polymorphic homozygote and heterozygosity. The genetic diversity was observed by using the RFLP band analysis and four different groups of individuals were identified with the p-values of 0, 0.033, 0.05 and 1 respectively whereas different distances were found among the groups indicating the polymorphism among experimental individuals of glutamine synthetase gene. Conclusion: Herein, RAPD and RFLP analysis indicating the rich genetic diversity of this fish in the experimental ecological habitat and this would come helpful for the conservation of germplasm diversity and to support the sustainable breeding program of M. cuchia.

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Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus

Cited by 12 publications

References 48 publications

Molecular ontogeny of larval immunity in European eel at increasing temperatures

Molecular ontogeny of larval immunity in European eel at increasing temperatures

The char of migration inhibitory factor and Cathepsin B from Asian swamp eel (Monopterus albus) and their response to challenge with Aeromonas hydrophila

Assessment of Genetic Diversity of Freshwater Mud Eel (Monopterus cuchia) Using RAPD and RFLP Markers

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