Molecular cloning, mRNA expression and nutritional regulation of a Δ6 fatty acyl desaturase-like gene of mud crab, Scylla paramamosain

Lin, Zhideng; Hao, Meilin; Zhu, Deguang; Li, Shengkang; Wen, Xiaobo

doi:10.1016/j.cbpb.2017.03.004

Cited by 44 publications

(32 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present study, key genes involved in LC-PUFA biosynthesis, such as fad, elovl4, elovl, srebp1, hnf4a and rxr, were significantly down-regulated when dietary lipid level increased from 5•8 to 15•1 %, which was in agreement with previous studies in teleost fish when subjected to similar experimental conditions (38) . Moreover, some studies in crustaceans showed that fad and elovl4 expression were inhibited by the level of dietary fish oil (39)(40)(41) . This result may suggest that high dietary fish oil could inhibit the synthesis of LC-PUFA in hepatopancreas of crab or, alternatively, that low dietary LC-PUFA activates endogenous production of these key fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

et al. 2019

View full text Add to dashboard Cite

The regulation of lipogenesis and lipolysis mechanisms related to consumption of lipid has not been studied in swimming crab. The aims of the present study were to evaluate the effects of dietary lipid levels on growth, enzymes activities and expression of genes of lipid metabolism in hepatopancreas of juvenile swimming crab. Three isonitrogenous diets were formulated to contain crude lipid levels at 5·8, 9·9 and 15·1 %. Crabs fed the diet containing 15·1 % lipid had significantly lower growth performance and feed utilisation than those fed the 5·8 and 9·9 % lipid diets. Crabs fed 5·8 % lipid had lower malondialdehyde concentrations in the haemolymph and hepatopancreas than those fed the other diets. Highest glutathione peroxidase in haemolymph and superoxide dismutase in hepatopancreas were observed in crabs fed 5·8 % lipid. The lowest fatty acid synthase and glucose 6-phosphate dehydrogenase activities in hepatopancreas were observed in crabs fed 15·1 % lipid, whereas crabs fed 5·8 % lipid had lower carnitine palmitoyltransferase-1 activity than those fed the other diets. Crabs fed 15·1 % lipid showed lower hepatopancreas expression of genes involved in long-chain-PUFA biosynthesis, lipoprotein clearance, fatty acid uptake, fatty acid oxidation, lipid anabolism and lipid catabolism than those fed the other diets, whereas expression of some genes of lipoprotein assembly and fatty acid oxidation was up-regulated compared with crabs fed 5·8 % lipid. Overall, high dietary lipid level can inhibit growth, reduce antioxidant enzyme activities and influence lipid metabolic pathways to regulate lipid deposition in crab.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Scylla paramamosain (Crustacea: Decapoda: Portunidae: Syclla De Hann) is a commercially important mud crab distributed along the coast of southern China and other Indo‐Pacific countries (Lin, Hao, Zhu, Shengkang & Xiaobo, ; Wang, Sun & Zhu, ; Ye et al., ). The production of the mud crab in China reached 240,293 t in 2016, (China Fishery Statistical Yearbook, ).Because of its delicious meat and high nutritional and medicinal value, S. Paramamosain is very popular in China (Wang et al., ).…”

Section: Introductionmentioning

confidence: 99%

“Butter Crab”: an environment‐induced phenotypic variation of Scylla paramamosain with special nutrition and flavour

et al. 2018

View full text Add to dashboard Cite

An "abnormal" female Scylla paramamosain, named "Butter crab" (B_♀) is more popular than wild-type mud crab (CK) with people. Histologically, "butter crab" ovaries are partially degraded, with full of yellow oily substances in body. Composition of the essential nutritional components is quite similar in "butter crab" and CK. In a comparison of fatty acids, B_♀ is richer. A comparison of flavour amino acids and nucleotide in muscle showed that sweet amino acids and bitter amino acids in B_♀ is lower than CK_♀, but umami amino acids are significantly higher in B_♀. However, the umami amino acid content in the gonads is significantly less in B_♀ than CK_♀. Moreover, umami nucleotides in B_♀ muscle is significantly higher. From the perspective of EUC evaluation, the umami taste of CK_ ♀ gonads is strongest, followed by B_ ♀ gonads. In the hepatopancreas, the umami taste ranks CK_♀> B_♀ >CK_♂, and there are no significant differences among muscles in the three groups. In conclusion, the characteristics of "butter crab" are more fragrant and nutritious, with sweet and umami taste. The article systematically scientifically analysed the nutritional composition and flavour characteristics of "butter crab", providing a novel insight for development of S. paramamosain industry. K E Y W O R D S"Butter crab", flavour, nutritional composition, Scylla paramamosain

show abstract

“…Desaturase enzymes 67 themselves can be split up in front-end and methyl-end (or w-end) desaturases, depending on the location of the 68 double bond insertion (21). For crustaceans, genes encoding for front-end desaturase and elongase enzymes were 69 so far mainly detected in decapods (22)(23)(24)(25)(26). Interestingly, a recent study discovered genes encoding w-end 70 desaturases in many aquatic invertebrates including some orders of copepods, challenging the current dogma that 71 de novo PUFA biosynthesis occurs exclusively in marine microbes (27).…”

mentioning

confidence: 99%

Fatty acid bioconversion in harpacticoid copepods in a changing environment: a transcriptomic approach

Boyen

Fink

Mensens

et al. 2019

Preprint

View full text Add to dashboard Cite

By 2100, global warming is predicted to significantly reduce the capacity of marine primary producers for long-chain polyunsaturated fatty acid (LC-PUFA) synthesis. Primary consumers such as harpacticoid copepods (Crustacea) might mitigate the resulting adverse effects on the food web by increased LC-PUFA bioconversion. Here, we present a highquality de novo transcriptome assembly of the copepod Platychelipus littoralis, exposed to changes in both temperature (+3°C) and dietary LC-PUFA availability. Using this transcriptome, we detected multiple transcripts putatively encoding for LC-PUFA-bioconverting front-end fatty acid desaturases and elongases, and performed phylogenetic analyses to identify their relationship with sequences of other (crustacean) taxa. While temperature affected the absolute fatty acid concentrations in copepods, LC-PUFA levels remained unaltered even when copepods were fed a LC-PUFAdeficient diet. While this suggests plasticity of LC-PUFA bioconversion within P. littoralis, none of the putative frontend desaturase or elongase transcripts were differentially expressed under the applied treatments. Nevertheless, the transcriptome presented here provides a sound basis for future ecophysiological research on harpacticoid copepods.

show abstract

Molecular cloning, mRNA expression and nutritional regulation of a Δ6 fatty acyl desaturase-like gene of mud crab, Scylla paramamosain

Cited by 44 publications

References 46 publications

Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

“Butter Crab”: an environment‐induced phenotypic variation of Scylla paramamosain with special nutrition and flavour

Fatty acid bioconversion in harpacticoid copepods in a changing environment: a transcriptomic approach

Contact Info

Product

Resources

About