Molecular cloning, nucleotide sequence and gene expression of a cytochrome P450 (CYP6F1) from the pyrethroid-resistant mosquito, Culex quinquefasciatus Say

Kasai, Shinji; Weerashinghe, Indira S.; Shono, Toshiyuki; Yamakawa, Minoru

doi:10.1016/s0965-1748(99)00114-9

Cited by 87 publications

(54 citation statements)

References 24 publications

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“…Aegypti, where the whole genome sequence is available, microarray of all P450 has been used to identify possible detoxification genes that are involved in the metabolism of insecticides and xenobiotics . However, for important pest species such as Helicoverpa armigera Hübner and Culex quinquefasciatus Say, where whole genome sequence is not available, isolation of P450 genes or construction of P450 cDNA library would be required before further examination of their function can be carried out (Kasai et al 2000, Komagata et al 2010, Zhou et al 2010a. As mentioned earlier, most cases of P450-mediated insecticide resistance involve family 6 P450.…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 Monooxygenases in the Cotton Bollworm (Lepidoptera: Noctuidae): Tissue Difference and Induction

Qiu¹,

Li²,

Tian³

et al. 2003

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Section: Discussionmentioning

confidence: 99%

Cytochrome P450 Monooxygenases in the Cotton Bollworm (Lepidoptera: Noctuidae): Tissue Difference and Induction

Qiu¹,

Li²,

Tian³

et al. 2003

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“…Two degenerate oligonucleotide primers (CYP6-F and CYP6-R, Table I) were designed for reverse transcription-polymerase chain reaction (RT-PCR) to amplify cytochrome P450 family 6 genes (Kasai et al, 2000). Two P450 clones representing two novel family 6 genes were obtained after cloning and sequencing (data not shown).…”

Section: Cloning Of the Full Cdnasmentioning

confidence: 99%

Characterization and Induction of Two Cytochrome P450 Genes, CYP6AE28 and CYP6AE30, in Cnaphalocrocis medinalis: Possible Involvement in Metabolism of Rice Allelochemicals

Liu

Yang

2010

Zeitschrift Für Naturforschung C

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Two cDNAs specific for P450 genes, CYP6AE28 and CYP6AE30, have been isolated from the rice leaf folder Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae). Both cDNApredicted proteins have 504 amino acid residues in length, but with molecular masses of 60177 Dalton for CYP6AE28 and 60020 Dalton for CYP6AE30, and theoretical pI values of 8.49 for CYP6AE28 and 8.56 for CYP6AE30, respectively. Both putative proteins contain the conserved structural and functional domains characteristic of all CYP6 members. CYP6AE28 and CYP6AE30 show 52% amino acid identity to each other; both of them have 49 - 56% identities with CYP6AE1, Cyp6ae12, and CYP6AE14. Phylogenetic analysis showed that the two P450s are grouped in the lineage containing some of the CYP6AE members, CYP6B P450s and CYP321A1. The transcripts of CYP6AE28 and CYP6AE30 were found to be induced in response to TKM-6, a rice variety with high resistance to C. medinalis. The results suggest that the two P450s may play important roles in adaptation to the host plant rice. This is the first report of P450 genes cloned in C. medinalis

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“…A significant characteristic of insect P450s that is associated with enhanced metabolic detoxification of insecticides in insects is the constitutively increased P450 proteins and P450 activities that result from constitutively transcriptional overexpression of P450 genes in insecticide resistant insects (Carino et al, 1994;Feyereisen, 2005;Kasai et al, 2000; Scott, 1997, 1998). Cytochrome P450s constitute the largest gene superfamily found in all living organisms examined (Feyereisen, 2005).…”

Section: Introductionmentioning

confidence: 99%

Differential expression of CYP6A5 and CYP6A5v2 in pyrethroid‐resistant house flies, Musca domestica

Zhu

Liu

2007

Arch Insect Biochem Physiol

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Two cytochrome P450 alleles, CYP6A5 and CYP6A5v2, were isolated from a pyrethroid-resistant house fly stain, ALHF. The two alleles shared 98% similarity in amino acid sequence. To understand the importance of these two alleles in resistance and examine the expression profile of the two alleles between resistant and susceptible strains, quantitative real-time PCR (qRT-PCR) was performed and compared with the Northern blot analysis. We found that qRT-PCR was an efficient method to characterize the expression profiles between these two sequence-closely-related P450 genes between resistant and susceptible houses flies. One of them, CYP6A5v2, was constitutively overexpressed in ALHF house flies compared with susceptible house fly strains. Moreover, this gene was predominantly expressed in the abdominal tissues of ALHF, in which the primary detoxification organs of insects are located. However, there was no significant difference in the expression of CYP6A5 between ALHF and susceptible house flies. The genetic linkage analysis was conducted to determine the possible link between the constitutively overexpressed CYP6A5v2 and insecticide resistance. CYP6A5v2 was mapped on autosome 5, which is correlated with the linkage of resistance in ALHF. Taken together, the study suggests the importance of CYP6A5v2 in increasing metabolic detoxification of insecticides in ALHF. The distinct expression of CYP6A5 and CYP6A5v2 in resistant and susceptible house flies implies the functional difference of theses two genes in house flies and suggests that they are two recently diverged P450 genes presented in a single organism.

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Molecular cloning, nucleotide sequence and gene expression of a cytochrome P450 (CYP6F1) from the pyrethroid-resistant mosquito, Culex quinquefasciatus Say

Cited by 87 publications

References 24 publications

Cytochrome P450 Monooxygenases in the Cotton Bollworm (Lepidoptera: Noctuidae): Tissue Difference and Induction

Cytochrome P450 Monooxygenases in the Cotton Bollworm (Lepidoptera: Noctuidae): Tissue Difference and Induction

Characterization and Induction of Two Cytochrome P450 Genes, CYP6AE28 and CYP6AE30, in Cnaphalocrocis medinalis: Possible Involvement in Metabolism of Rice Allelochemicals

Differential expression of CYP6A5 and CYP6A5v2 in pyrethroid‐resistant house flies, Musca domestica

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