Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in <i>Brassica rapa</i>

Toriyama, Kinya; Hanaoka, Kazunori; Okada, Takashi; Watanabe, Masao

doi:10.1016/s0014-5793(98)00174-4

Cited by 42 publications

(20 citation statements)

References 32 publications

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“…Finally, thioredoxin-like molecules have been found on extracellular pollen and detected in self-incompatibility reactions (88,156). A thioredoxin activity linked to the C terminus of the S gene product seems to be responsible for selfincompatibility.…”

Section: New Functions For Thioredoxins In Plantsmentioning

confidence: 99%

Plant Thioredoxin Systems Revisited

Schürmann

Jacquot

2000

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

376

267

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s Abstract Thioredoxins, the ubiquitous small proteins with a redox active disulfide bridge, are important regulatory elements in plant metabolism. Initially recognized as regulatory proteins in the reversible light activation of key photosynthetic enzymes, they have subsequently been found in the cytoplasm and in mitochondria. The various plant thioredoxins are different in structure and function. Depending on their intracellular location they are reduced enzymatically by an NADP-dependent or by a ferredoxin (light)-dependent reductase and transmit the regulatory signal to selected target enzymes through disulfide/dithiol interchange reactions. In this review we summarize recent developments that have provided new insights into the structures of several components and into the mechanism of action of the thioredoxin systems in plants.

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Section: New Functions For Thioredoxins In Plantsmentioning

confidence: 99%

Plant Thioredoxin Systems Revisited

Schürmann

Jacquot

2000

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

376

267

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“…The PCP-A1 was a cysteine-rich basic protein, and a member of a large protein family, the PCP family (Doughty et al 1998). A cDNA clone homologous to the PCP-A1 gene was also isolated from a cDNA library of immature anthers with polyclonal antiserum against the PCPs (Toriyama et al 1998). By using a surface plasmon resonance sensor (BIAcore) along with chromatographic methods, many PCP-like molecules, which can interact with SLG, were identified in the proteins extracted from the pollen coat, though their interaction was not S haplotype-specific, like PCP-A1 ).…”

Section: Identification and Functional Characterization Of Male S Detmentioning

confidence: 99%

Recent Progresses on Self-incompatibility Research in Brassica Species

et al. 2003

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Many hermaphrodite plant species have evolved mechanisms to prevent self-fertilization. One such mechanism is self-incompatibility (SI), which is defined as the inability of a fertile hermaphrodite plant to produce zygotes after self-pollination. SI prevents self-fertilization by rejecting pollen from plants with the same genotype. The SI system in Brassica is controlled sporophytically by multiple alleles at a single locus, designated as S, and involves cell-cell communication between male and female. When the S phenotype of the pollen is the same as that of the stigma, pollen germination and/or pollen tube penetration are disturbed on the papilla cells. On the female side, two genes (SLG and SRK) located at the S locus, are involved in the SI reaction. SLG is a secreted glycoprotein expressed abundantly in the papilla cell, and SRK is a membrane-spanning receptor-like serine/ threonine kinase whose extracellular domain is highly similar to SLG. Gain-of-function experiments have demonstrated that SRK solely determines S haplotypespecificity of the stigma, while SLG enhances the recognition reaction of SI. The sequence analysis of the S locus genomic region of Brassica campestris (syn. rapa) has led to the identification of an anther-specific gene, designated as SP11, which encodes a small cysteine-rich basic protein. Pollination bioassay and gain-of-function experiments have indicated that SP11 is the male S determinant. When the sequence of SP11 was aligned, six cysteine residues were found to be completely conserved among alleles. These conserved cysteine residues could be important for the tertiary structure of SP11. Recent biochemical analysis has suggested that SP11 operates as a sole ligand to activate its cognate SRK specifically. Because the activity of the S allele is controlled sporophytically, dominance relationships influence the ultimate phenotype of both the stigma and pollen. Molecular analysis has demonstrated that the dominance relationships between S alleles in the stigma were determined by SRK itself, but not by the relative expression level. In contrast, in the pollen, the expression of SP11 from the recessive S allele was specifically suppressed in the S heterozygote, suggesting that the dominance relationships in pollen were determined by the expression level of SP11.

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“…Analysis of PCPs, successfully lead to identification of the male S determinant (Heslop-Harrison 1975). Initially, SLG-interactive PCPs were searched (Doughty et al 1993), and these interactive molecules were then characterized as cysteine-rich small proteins (Hiscock et al 1995;Stanchev et al 1996;Toriyama et al 1998;Doughty et al 1998;Takayama et al 2000a). The cysteinerich small proteins were shown to be a < 10kDa basic pollen coat proteins (PCP) by an elegant in vitro bioassay in which PCPs were isolated and fractionated (Stephenson et al 1997).…”

Section: Si Recognition Genes Identified From Anthermentioning

confidence: 99%

Molecular Genetic Aspects of Self-incompatibility in Brassicaceae

Jung¹,

Ahmed²,

Park³

et al. 2013

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Department of Crop Science, Chungbuk National University, 52 Naesudongro, Heungdokgu, Cheongju 361-763, Republic of Korea ABSTRACT Molecular genetic studies of self-incompatibility (SI) are the most accentuating part in the way of advancement of reproductive mechanisms in flowering plants. In the Brassicaceae plants, self-incompatibility has been mapped genetically to a single chromosomal location where several closely linked genes have been identified. Recently, various studies have provided a novel insight into the basis of specificity in the S-receptor kinase (SRK) and S-locus protein 11 or S-locus Cysteine-rich (SP11/SCR) interaction, the nature of the signaling cascade that culminates in the inhibition of 'self' pollen, and the physiological and morphological changes that are associated with transitions between the outbreeding and inbreeding modes of mating in the Brassicaceae. In this review, we discuss the current view of the molecular genetic aspects of the self-incompatibility in Brassicaceae.

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Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa

Cited by 42 publications

References 32 publications

Plant Thioredoxin Systems Revisited

Plant Thioredoxin Systems Revisited

Recent Progresses on Self-incompatibility Research in Brassica Species

Molecular Genetic Aspects of Self-incompatibility in Brassicaceae

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