Molecular cloning of a cDNA encoding human SPH-binding factor, a conserved protein that binds to the enhancer-like region of the U6 small nuclear RNA gene promoter

Gómez-Rincón, Julio César; Engler, Sarah K.; Hargrove, Brian W.; Kunkel, Gary R.

doi:10.1093/nar/26.21.4846

Cited by 27 publications

(35 citation statements)

References 3 publications

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“…The C2 is shown as the retarded probe at the origin of the gel, which was described previously. 17 Similar results were obtained with nuclear extracts of PC3 cells (data not shown). Because Staf binding activity increased in nuclear extracts of cisplatin-treated cells, we investigated whether the expression of ZNF 76 and/or ZNF 143 is cisplatin-inducible.…”

Section: Expression Of Znf 143 Is Cisplatin-induciblesupporting

confidence: 81%

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ZNF143 activates gene expression in response to DNA damage and binds to cisplatin‐modified DNA

Ishiguchi

Izumi

Torigoe

et al. 2004

Intl Journal of Cancer

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We have identified a cisplatin-inducible gene, the mitochondrial ribosomal protein S11 (MRP S11) gene, by means of mRNA differential display. Functional analysis of the MRP S11 promoter showed that a Staf binding site in the promoter is required for both basal promoter activity and cisplatin-inducible activity. We also found that Cisplatin is a widely used anticancer agent, and DNA damage is believed to be the main basis of the antitumor effect of cisplatin. Since cisplatin resistance is a major obstacle to the treatment of solid tumors, understanding the molecular basis of the DNA damage response and of cisplatin resistance is important for clinical protocols. Cisplatin resistance is thought to involve several mechanisms, including increased drug efflux, the presence of cellular thiols, increased nucleotide excision repair activity and decreased mismatch repair activity. [1][2][3][4] In addition, several mechanisms are thought to contribute to the DNA damage response associated with cisplatin resistance. To understand fully these molecular mechanisms, it is desirable to identify and characterize all the transcription factors that are activated by DNA damage. We have previously shown that transcription factors such as Y-box binding protein (YB-1) and the CCAAT-binding transcription factor 2 (CTF2) are overexpressed in human cancer cell lines resistant to cisplatin. 5,6 YB-1 has been shown to bind preferentially to cisplatin-modified DNA 5 and is activated by nuclear translocation and by transcriptional mechanisms. Oct1 participates in the cellular response to DNA damage, 7,8 and its induction involves posttranscriptional mechanisms. Both c-Myc and AP-1 are also activated and involved in cellular responses to cisplatin. 8 One cisplatininducible cDNA clone has been shown to encode the vacuolar H ϩ -ATPase subunit c (ATP6L), 9,10 and analysis of its expression revealed that both Sp1 and Oct1 are critical for its activation by anticancer agents. 11 In order to identify novel mechanisms involved in the DNA damage response, we have used differential display to compare gene expression between control and cisplatin-treated cells. Here, we characterize one cisplatin-inducible gene, MRP S11, whose product, MRP S11, is a component of the ribosomal small subunit and binds to 12S rRNA. 12 MRP S11 appears to have RNA binding but not DNA binding activity. We have isolated the promoter region of MRP S11 in order to identify its transcriptional activators. Functional analysis revealed a critical Staf binding site in the core of the promoter. The zinc finger transcription factor, Staf, originally identified in the frog, is involved in transcriptional regulation of snRNA type and mRNA promoters transcribed either by RNA polymerase II or III. 13 Two human homologues of Staf, ZNF 76 and ZNF 143, have been isolated. 14 Here we show that activation of the transcriptional activator ZNF 143 upregulates the transcription of MRP S11. We present evidence that ZNF 143, but not ZNF 76, is induced by DNA damage. We further demonstrate that ZN...

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Section: Expression Of Znf 143 Is Cisplatin-induciblesupporting

confidence: 81%

“…Kunkel. 17 An antimethyl-CpG-binding protein 2 (MeCP2) antibody was purchase from Santa Cruz Biotechnology (Santa Cruz, CA).…”

Section: Cell Culture Drugs Irradiation and Antibodymentioning

confidence: 99%

ZNF143 activates gene expression in response to DNA damage and binds to cisplatin‐modified DNA

Ishiguchi

Izumi

Torigoe

et al. 2004

Intl Journal of Cancer

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“…The human ZNF143 is a Staf family transcription factor known to control the expression of tRNA sec and snRNAs, such as U1 and U6 (24,25). The Xenopus homologue of ZNF143 is called Staf and has been well characterized (18 -20).…”

Section: Cis-acting Elements Up-regulating the Ccta Gene Promotermentioning

confidence: 99%

“…CAE1/CAE2-binding Factor in HeLa Cell Is ZNF143-ZNF143 (hSBF) is known to be contained in HeLa cells and activate transcription of the human U6 gene (25). To determine whether the CAE1/CAE2-binding factor is ZNF143, we prepared a rabbit antibody against ZNF143 for EMSA supershift analysis.…”

Section: Figmentioning

confidence: 99%

“…There are seven zinc-finger repeats for DNA binding in the middle of the Staf molecule and the amino-terminal portion contains a transcription activation domain. Two mammalian homologues of Staf, ZNF76 and ZNF143, have been isolated (21,22) and shown to recognize very similar sequences (23)(24)(25). ZNF76 and ZNF143 are considered to play basically the same role as Staf, whereas the expression levels in various tissues are different between the two homologues.…”

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confidence: 99%

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Transcriptional Regulation of the Mouse Cytosolic Chaperonin Subunit Gene Ccta/t-Complex Polypeptide 1 by Selenocysteine tRNA Gene Transcription Activating Factor Family Zinc Finger Proteins

Yokota

Yanagi

Yura

2000

Journal of Biological Chemistry

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The chaperonin containing t-complex polypeptide 1 (CCT) is a molecular chaperone assisting in the folding of proteins in eukaryotic cytosol, and the Ccta (encoding the ␣ subunit of CCT)/t-complex polypeptide 1 gene encodes the ␣ subunit of CCT. We show here that transcription of the mouse Ccta gene is regulated by selenocysteine tRNA gene transcription activating factor (Staf) family zinc-finger transcription factors ZNF143 and ZNF76. Reporter gene assay using HeLa cells indicated that the Ccta gene promoter contains two 18-base pairlong cis-acting elements with similar sequences at -70 and -20 base pairs (designated CCT ␣ subunit gene transcription activating element 1 (CAE1) and CAE2, respectively). By yeast one-hybrid screening of CAE1-binding factors, we isolated human ZNF143, which is known to activate transcription of selenocysteine tRNA and small nuclear RNA genes. DNA binding domains of ZNF143 and ZNF76 produced in E. coli recognized CAE1 and CAE2 elements in electrophoretic mobility shift assay. HeLa cell nuclear extract contained a protein that specifically binds to CAE1 and CAE2 and recognized by anti-ZNF143 antibody. Transcription from a minimal Ccta promoter containing CAE2 element in HeLa cells was enhanced by overexpression of full-length ZNF143 and ZNF76 but inhibited by that of their DNA binding domains alone. These results demonstrate that the Staf family proteins control transcription of at least one of the chaperone-encoding genes besides that of tRNA and small nuclear RNA genes. These RNA and chaperone genes are suggested to be coregulated to facilitate synthesis of mature proteins during active cell growth.

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The RNA polymerase III transcription apparatus11Edited by P. E. Wright

Geiduschek

Kassavetis

2001

Journal of Molecular Biology

349

326

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Molecular cloning of a cDNA encoding human SPH-binding factor, a conserved protein that binds to the enhancer-like region of the U6 small nuclear RNA gene promoter

Cited by 27 publications

References 3 publications

ZNF143 activates gene expression in response to DNA damage and binds to cisplatin‐modified DNA

ZNF143 activates gene expression in response to DNA damage and binds to cisplatin‐modified DNA

Transcriptional Regulation of the Mouse Cytosolic Chaperonin Subunit Gene Ccta/t-Complex Polypeptide 1 by Selenocysteine tRNA Gene Transcription Activating Factor Family Zinc Finger Proteins

The RNA polymerase III transcription apparatus11Edited by P. E. Wright

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