1998
DOI: 10.1073/pnas.95.2.472
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Molecular cloning of a human cDNA encoding β-1,4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc β-1,4-galactosyltransferase

Abstract: A cDNA encoding a ␤-1,4-galactosyltransferase named ␤-1,4-GalT II was cloned from a cDNA library of the human breast tumor cell line, MRK-nu-1. Initially, a 860-bp PCR fragment was obtained from MRK-nu-1 mRNA by 3-rapid amplification of cDNA ends by using two nested degenerate oligonucleotide primers based on a highly conserved amino acid sequence found in the catalytic domain of mammalian ␤-1,4-galactosyltransferases and Lymnaea stagnalis ␤-1,4-N-acetylglucosaminyltransferase (␤-1,4-GlcNAcT), both of which ut… Show more

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Cited by 122 publications
(78 citation statements)
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“…The ␤-1,4-GalT V gene is expressed at high levels in most human tissues (21), and its expression increases upon malig- nant transformation of cells (17,20). Because the reduction in the expression level of the ␤-1,4-GalT V gene in SH-SY5Y cells resulted in the decreased galactosylation of highly branched N-glycans, the ␤-1,4-GalT V is considered to be involved in the expression of highly branched N-glycans, which are involved in abnormal growth and metastasis of cancer cells (11,13).…”
Section: Discussionmentioning
confidence: 99%
“…The ␤-1,4-GalT V gene is expressed at high levels in most human tissues (21), and its expression increases upon malig- nant transformation of cells (17,20). Because the reduction in the expression level of the ␤-1,4-GalT V gene in SH-SY5Y cells resulted in the decreased galactosylation of highly branched N-glycans, the ␤-1,4-GalT V is considered to be involved in the expression of highly branched N-glycans, which are involved in abnormal growth and metastasis of cancer cells (11,13).…”
Section: Discussionmentioning
confidence: 99%
“…Characterization of an Insect ␤4-Galactosyltransferase Family Member-The cDNA described in this study was isolated on the basis of short, conserved amino acid sequences in ␤4-galactosyltransferase family members, which were noted in the literature by several different investigators during the late 1990s (75)(76)(77)(78)(79)(80). Degenerate oligonucleotides encoding four of these conserved amino acid sequences (Table I) were synthesized and used as primers for nested PCRs with cDNA prepared from T. ni membrane-bound RNA, as described under "Experimental Procedures."…”
Section: Isolation Andmentioning
confidence: 99%
“…preparation (Sigma) was directly used as ␤4Gal-TI (27). ␤4Gal-TV (37) was cloned and expressed in COS-1 cells as described previously (27). For comparing the enzymatic activities of different ␤4Gal-T samples, the final concentration of ␤4Gal-TI, -TII, -TIII, -TIV, and -TV was adjusted to 38.0 nmol/h/ml as measured using 0.5 mM GlcNAc␤13p-nitrophenol (Sigma) as an acceptor.…”
Section: Isolation and Expression Of Cdna Encoding Ignt-mentioning
confidence: 99%