Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential

Mardassi, Boutheina Ben Abdelmoumen; Khiari, A. Béjaoui; Oussaief, Lassad; Landoulsi, Ahmed; Brik, C.; Mlik, Béhija; Amouna, F.

doi:10.1016/j.vetmic.2006.08.008

Cited by 12 publications

(4 citation statements)

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“…Immunoscreening and isolation of M . meleagridis -specific DNA fragments from recombinant λ phages were performed as previously described [ 29 ]. In order to favor selection of DNA fragments that encode surface antigens, we screened the M .…”

Section: Methodsmentioning

confidence: 99%

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Mm19, a Mycoplasma meleagridis Major Surface Nuclease that Is Related to the RE_AlwI Superfamily of Endonucleases

Yacoub

Mardassi

2016

PLoS ONE

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Mycoplasma meleagridis infection is widespread in turkeys, causing poor growth and feathering, airsacculitis, osteodystrophy, and reduction in hatchability. Like most mycoplasma species, M. meleagridis is characterized by its inability to synthesize purine and pyrimidine nucleotides de novo. Consistent with this intrinsic deficiency, we here report the cloning, expression, and characterization of a M. meleagridis gene sequence encoding a major surface nuclease, referred to as Mm19. Mm19 consists of a 1941- bp ORF encoding a 646-amino-acid polypeptide with a predicted molecular mass of 74,825 kDa. BLASTP analysis revealed a significant match with the catalytic/dimerization domain of type II restriction enzymes of the RE_AlwI superfamily. This finding is consistent with the genomic location of Mm19 sequence, which dispalys characteristics of a typical type II restriction-modification locus. Like intact M. meleagridis cells, the E. coli-expressed Mm19 fusion product was found to exhibit a nuclease activity against plasmid DNA, double-stranded DNA, single-stranded DNA, and RNA. The Mm19-associated nuclease activity was consistently enhanced with Mg2+ divalent cations, a hallmark of type II restriction enzymes. A rabbit hyperimmune antiserum raised against the bacterially expressed Mm19 strongly reacted with M. meleagridis intact cells and fully neutralized the surface-bound nuclease activity. Collectively, the results show that M. meleagridis expresses a strong surface-bound nuclease activity, which is the product of a single gene sequence that is related to the RE_AlwI superfamily of endonucleases.

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Section: Methodsmentioning

confidence: 99%

“…Genomic DNA was extracted from M . meleagridis as described elsewhere [ 29 , 32 ]. Specific primers; Mm19-BamF ( ) and Mm19-NotR ( ) were used to amplify the full-length Mm19 coding sequence.…”

Section: Methodsmentioning

confidence: 99%

Mm19, a Mycoplasma meleagridis Major Surface Nuclease that Is Related to the RE_AlwI Superfamily of Endonucleases

Yacoub

Mardassi

2016

PLoS ONE

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“…Mycoplasma meleagridis is widespread in turkey flocks, causing poor growth, air sacculitis, osteodystrophy, and immunosuppression ( 1 , 2 ). A recent work provided the first evidence that M. meleagridis could also establish a natural infection in chickens ( 3 ).…”

Section: Genome Announcementmentioning

confidence: 99%

“…A recent work provided the first evidence that M. meleagridis could also establish a natural infection in chickens ( 3 ). Moreover, a number of antigens appear to be shared between M. meleagridis and the more important poultry mycoplasmas ( Mycoplasma gallisepticum and Mycoplasma synoviae ) resulting in cross-reactivity that complicates serological investigations ( 2 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Genome Sequence of a Mycoplasma meleagridis Field Strain

et al. 2016

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Mycoplasma

Brown¹,

May²,

Bradbury³

et al. 2015

Bergey's Manual of Systematics of Archaea and Bacteria

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My.co.plas'ma. Gr. masc. n. myces a fungus; Gr. neut. n. plasma something formed or molded, a form; N.L. neut. n. Mycoplasma fungus form. Tenericutes / Mollicutes / Mycoplasmatales / Mycoplasmataceae / Mycoplasma Pleomorphic cells, 300–800 nm in diameter, varying in shape from spherical, ovoid or flask‐shaped, or twisted rods, to slender branched filaments ranging in length from 50 to 500 nm. Cells lack a cell wall and are bounded by a single plasma membrane. Gram‐stain‐negative due to the absence of a cell wall. Some have a complex internal cytoskeleton. Some have a specific tip structure that mediates attachment to host cells or other surfaces. Usually nonmotile , but gliding motility has been demonstrated in some species. Aerobic or facultatively anaerobic . Optimum growth at 37°C is common, but permissive growth temperatures range from 20 to 45°C. Chemo‐organotrophic , usually using either sugars or arginine as the major energy source. Require cholesterol or related sterols for growth. Colonies are usually less than 1 mm in diameter. The typical colony has a fried‐egg appearance . The genome size of species examined ranges from 580 kbp to about 1350 kbp . The codon UGA encodes tryptophan in all species examined. Commensals or pathogens in a wide range of vertebrate hosts. DNA G + C content ( mol %): 23–40. Type species : Mycoplasma mycoides (Borrel, Dujardin‐Beaumetz, Jeantet and Jouan 1910) Freundt 1955, 73 ( Asterococcus mycoides Borrel, Dujardin‐Beaumetz, Jeantet and Jouan 1910, 179).

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Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential

Cited by 12 publications

References 33 publications

Mm19, a Mycoplasma meleagridis Major Surface Nuclease that Is Related to the RE_AlwI Superfamily of Endonucleases

Mm19, a Mycoplasma meleagridis Major Surface Nuclease that Is Related to the RE_AlwI Superfamily of Endonucleases

Genome Sequence of a Mycoplasma meleagridis Field Strain

Mycoplasma

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