1997
DOI: 10.1074/jbc.272.48.30329
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Molecular Cloning of a New Aquaporin from Rat Pancreas and Liver

Abstract: A new water channel (aquaporin-8, gene symbol AQP8) was isolated from rat pancreas and liver by homology cloning. Ribonuclease protection assay showed intense expression of the gene in pancreas and liver, less intense in colon and salivary gland, and negligible in other organs. The full-length cDNA was obtained by ligation of ϳ1.4-kilobase (kb) cDNA isolated from the rat liver cDNA library to ϳ0.5 kb of the 5-end fragment obtained by the rapid amplification of cDNA ends method. A major transcript of ϳ1.45 kb w… Show more

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Cited by 168 publications
(134 citation statements)
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“…1A, immunoblotting analysis for AQP8 detected a band of ϳ34 kDa in total liver and hepatocyte membranes. An identical band in location was obtained for colon mucosa (another rat AQP8 message-expression tissue) (7,8), whereas no band was detected for kidney or lung tissues (negative controls for rat AQP8 expression) (7,8). The 34 kDa band was absent when AQP8 antibody was preabsorbed with a molar excess of the immunizing peptide (not shown).…”
Section: Aquaporin-8 Protein Expression In Hepatocytesmentioning
confidence: 71%
See 1 more Smart Citation
“…1A, immunoblotting analysis for AQP8 detected a band of ϳ34 kDa in total liver and hepatocyte membranes. An identical band in location was obtained for colon mucosa (another rat AQP8 message-expression tissue) (7,8), whereas no band was detected for kidney or lung tissues (negative controls for rat AQP8 expression) (7,8). The 34 kDa band was absent when AQP8 antibody was preabsorbed with a molar excess of the immunizing peptide (not shown).…”
Section: Aquaporin-8 Protein Expression In Hepatocytesmentioning
confidence: 71%
“…AQP8 has been identified recently (7)(8)(9), and its transcript has been found to be present in rat liver (7,8). In situ hybridization studies suggest that AQP8 mRNA is present in hepatocytes; nevertheless, studies on protein expression were not undertaken (7).…”
Section: Discussionmentioning
confidence: 99%
“…AQP 9 is the most recently cloned water channel and is most similar to AQP 3 and 7 in that it also permits glycerol transport. It is expressed in leukocytes and liver and is weakly expressed in the heart, kidney and small intestine (224,237,238). The blastocyst provides a comparatively simple system from which fundamental information on AQP gene expression and function can be obtained.…”
Section: Aquaporinsmentioning
confidence: 99%
“…AQP6 is expressed in kidney but exhibits extremely low water permeability, and its distribution has not been defined (35). The cDNA encoding AQP8 was recently isolated from testis, pancreas, liver, and other tissues (36).…”
Section: Minireview: Aquaporins Blueprints For Cellular Plumbing Sysmentioning
confidence: 99%