Molecular cloning of a novel gene involved in serotonin receptor‐mediated signal transduction in rat stomach

Ohya, Susumu; Takii, Takemasa; Yamazaki, Hiroko; Matsumori, Miki; Onozaki, Kikuo; Watanabe, Minoru; Imaizumi, Yuji

doi:10.1016/s0014-5793(96)01485-8

Cited by 4 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The amino acid sequences corresponding to four variants of Drosophila Shal (dShal) and Kv4.3 from rat brain (Kv4.3M and Kv4.3S) and vas deferens (Kv4.3L) were aligned. The sequences, Kv4.3M, Kv4.3S and dShal have been reported previously by Serodio et al (1996) [10], Tsaur et al (1997) [15]and Wei et al (1990) [32], respectively. Dashes indicate gaps introduced into the sequence to improve alignment.…”

Section: Resultssupporting

confidence: 72%

“…Expression of a splice variant of Kv4.3S isolated from rat hippocampus [15]with an open reading frame of 611 amino acids. RT‐PCRs were performed with a specific degenerate PCR primer for 40 cycles.…”

Section: Resultsmentioning

confidence: 99%

“…Human embryonal kidney cell line (HEK293) was obtained from Health Science Research Resources Bank (HSRRB) and maintained in Minimum Essential Medium (Gibco BRL) supplemented with 10% heat‐inactivated fetal calf serum, penicillin (100 units/ml), streptomycin (100 μg/ml). A mammalian expression vector, pcDNA3.1 (Invitrogen), was used for stable transfection of HEK239 cells with Kv4.3 by calcium phosphate precipitation and then G418 (Gibco BRL) resistance cells were selected as previously described [15]and identified by Northern blot hybridization.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K⁺ channel α‐subunit, Kv4.3 in the rat

et al. 1997

Self Cite

View full text Add to dashboard Cite

We describe here (1) the heterogeneous expression of Ca 2+ -independent transient (A-type) K + channel K K-subunits (Kv1.4, Kv3.3, Kv3.4, Kv4.2 and Kv4.3) in rat smooth muscle, heart and brain, (2) the molecular cloning and tissue distribution of a novel alternatively spliced variant of an A-type K + channel K K-subunit, Kv4.3, and (3) The longer splice variant is very weakly expressed in brain, but is the major product in heart. z 1997 Federation of European Biochemical Societies.

show abstract

Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K⁺ channel α‐subunit, Kv4.3 in the rat

et al. 1997

Self Cite

View full text Add to dashboard Cite

show abstract

“…Oocytes were incubated at 20°C in standard ND96 solution (in mM: 96 NaCl, 2 KCl, 1.8 CaCl 2 , 1 MgCl 2 , and 5 HEPES, pH 7.4, adjusted with NaOH) supplemented with antibiotics to permit channel expression. One or 2 days after the injection of cRNA, oocytes were voltage-clamped using two microelectrodes (22). Membrane currents were recorded using an amplifier (CEZ-1250; Nihon Koden, Tokyo, Japan) and a pen recorder (FBR-252A; DKK-TOA, Tokyo, Japan).…”

Section: Rna Extraction Rt-pcr and Real Time Pcr-totalmentioning

confidence: 99%

Involvement of Dominant-negative Spliced Variants of the Intermediate Conductance Ca2+-activated K+ Channel, KCa3.1, in Immune Function of Lymphoid Cells

Ohya

Niwa

Yanagi

et al. 2011

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

“…Each sequence of rK v4.3 mutants [rK v4.3T53A, rKv4.3S516A (C1), rKv4.3S550A (C2), and rK v4.3 S516A and S550A (C1 and C2)] was confirmed by DNA sequencing. HEK-293 cell lines were used for stable transfection with the calcium phosphate coprecipitation technique, and then G418 (GIBCO-BRL) resistance cells were selected as previously reported (27).…”

Section: Expression Of Kv43 In Hek-293 Cellsmentioning

confidence: 99%

Regulation of K_v4.3 currents by Ca²⁺/calmodulin-dependent protein kinase II

Sergeant

Ohya²,

Reihill³

et al. 2005

American Journal of Physiology-Cell Physiology

Self Cite

View full text Add to dashboard Cite

The voltage-dependent K ϩ channel 4.3 (Kv4.3) is one of the major molecular correlates encoding a class of rapidly inactivating K ϩ currents, including the transient outward current in the heart (I to) and A currents (IA) in neuronal and smooth muscle preparations. Recent studies have shown that I to in human atrial myocytes and IA in murine colonic myocytes are modulated by Ca 2ϩ /calmodulin-dependent protein kinase II (CaMKII); however, the molecular target of CaMKII in these studies has not been elucidated. We performed experiments to investigate whether CaMKII could regulate K v4.3 currents directly. Inclusion of the autothiophosphorylated form of CaMKII in the patch pipette (10 nM) prolonged K v4.3 currents such that the time required to reach 50% inactivation from peak more than doubled, with positive shifts in voltage dependence of both activation and inactivation. In contrast, the rate of recovery from inactivation was accelerated under these conditions. CaMKII-inhibitory peptide or KN-93 produced effects opposite to that above; thus the rate of inactivation was increased, and recovery from inactivation decreased. A number of mutagenesis experiments were conducted on the three candidate CaMKII consensus sequence sites on the channel. Mutations at S550A, located at the COOH-terminal region of the channel, resulted in currents that inactivated more rapidly but recovered from inactivation at a slower rate than that of wild-type controls. In addition, these currents were unaffected by dialysis with either autothiophosphorylated CaMKII or the specific inhibitory peptide of CaMKII, suggesting that CaMKII slows the inactivation and accelerates the rate of recovery from inactivation of Kv4.3 currents by a direct effect at S550A, located at the COOH-terminal region of the channel. transient outward current; potassium channel; inactivation; phosphorylation

show abstract

Molecular cloning of a novel gene involved in serotonin receptor‐mediated signal transduction in rat stomach

Cited by 4 publications

References 27 publications

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K⁺ channel α‐subunit, Kv4.3 in the rat

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K⁺ channel α‐subunit, Kv4.3 in the rat

Involvement of Dominant-negative Spliced Variants of the Intermediate Conductance Ca2+-activated K+ Channel, KCa3.1, in Immune Function of Lymphoid Cells

Regulation of K_v4.3 currents by Ca²⁺/calmodulin-dependent protein kinase II

Contact Info

Product

Resources

About

Molecular cloning of a novel gene involved in serotonin receptor‐mediated signal transduction in rat stomach

Cited by 4 publications

References 27 publications

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K+ channel α‐subunit, Kv4.3 in the rat

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K+ channel α‐subunit, Kv4.3 in the rat

Involvement of Dominant-negative Spliced Variants of the Intermediate Conductance Ca2+-activated K+ Channel, KCa3.1, in Immune Function of Lymphoid Cells

Regulation of Kv4.3 currents by Ca2+/calmodulin-dependent protein kinase II

Contact Info

Product

Resources

About

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K⁺ channel α‐subunit, Kv4.3 in the rat

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K⁺ channel α‐subunit, Kv4.3 in the rat

Regulation of K_v4.3 currents by Ca²⁺/calmodulin-dependent protein kinase II