Molecular Cloning of a Novel Isoform of Diphosphoinositol Polyphosphate Phosphohydrolase: A Potential Target of Lithium Therapy

Hua, Len

doi:10.1016/s0893-133x(00)00233-5

Cited by 21 publications

(22 citation statements)

References 43 publications

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“…This includes inositol monophosphatase (Shamir et al 1998), diphosphoinositol polyphosphate phosphohydrolase II (Hua et al 2001), sodium/myo-inositol cotransporter (Lubrich and van Calker 1999), aldolase A (Hua et al 2000) and C isozymes (Hua and Li, unpublished), prolyl oligopeptidase (Williams et al 1999), and CD151 (present study). Such a cluster of changes is unlikely a chance event.…”

Section: Discussionmentioning

confidence: 66%

“…This process resulted in the amplification of three PCR fragments, two of which (5R1, 5R2) were later proved to be partial clones of diphosphoinositol polyphosphate phosphohydrolase II (Hua et al 2001). In an initial characterization of the other PCR fragment, designated 5R3, the tissue distribution of its corresponding mRNA was examined.…”

Section: Pcr Amplification Of a Novel Cdna And Regulation By Mood Stamentioning

confidence: 99%

“…These novel lithium-regulated targets included 2 Ј , 3 Ј -cyclic nucleotide 3 Ј -phosphodiesterase type II (Wang and Young 1996), polyomavirus enhancer-binding protein 2 ␤ , nitrogen permease regulator 2 (Wang et al 1999), aldolase A (Hua et al 2000), and diphosphoinositol polyphosphate phosphohydrolase II (Hua et al 2001). Unexpectedly, during the course of experiments amplifying the 5 Ј end of a putative lithium-regulated ddPCR fragment, we isolated another cDNA clone, a homolog of human/mouse transmembrane-4-superfamily (TM4SF) protein, CD151.…”

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confidence: 99%

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Tetraspan Protein CD151 A Common Target of Mood Stabilizing Drugs?

Hua

Green

Wong

et al. 2001

Neuropsychopharmacology

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The latency in onset of antimanic and mood stabilizing effects of lithium suggest that long-term neuronal adaptations mediated by changes in gene expression may be important to the therapeutic action of lithium treatment. Using differential display-polymerase chain reaction, several novel, hitherto unexpected lithium-regulated genes have been isolated, all of which would not have beenAlthough lithium is widely used in the treatment of acute mania and the prophylaxis for bipolar affective disorder, the cellular and molecular mechanisms underlying its therapeutic action remain unclear. However, significant progress has been made over the past decade with cumulative evidence indicating that modulation of postreceptor signaling mechanisms in critical regions of the brain may be essential to the mood stabilizing properties of this agent (reviewed in Jope 1999;Li et al. 2000;Manji and Lenox 1999).Many signal transduction cascades are known to induce long-term cellular responses through regulation of gene transcription. Moreover, the delayed onset of therapeutic action of lithium has led to the hypothesis that regulation of gene expression may be important for its mood stabilizing effects (Jope 1999;Li et al. 2000). In line with this hypothesis, several studies have demonstrated that chronic lithium treatment alters the expression of an array of genes, including G protein ␣ -subunits, adenylyl cyclase subtypes, neuropeptides (reviewed in Jope 1999;Li et al. 2000;Manji and Lenox 1999) NO . 5 et al. 2000). The modulatory effects of lithium on gene expression have been suggested to be mediated, at least in part, through the cAMP response element binding protein (CREB) or activator protein-1 (AP-1) transcriptional factor pathway (Ozaki and Chuang 1997;Asghari et al. 1998;Yuan et al. 1998). It is thus conceivable that other genes containing the consensus sequences for these transcription factors in their promoter region may also be the potential targets of lithium. Therefore, the identification of other lithium-regulated genes is important in better understanding the spectrum of cellular and molecular mechanisms that contribute to the therapeutic and/or side effects of this drug.Using differential display-polymerase chain reaction (ddPCR), several novel lithium-regulated genes have been identified that would not otherwise have been considered using a candidate gene approach. These novel lithium-regulated targets included 2 Ј , 3 Ј -cyclic nucleotide 3 Ј -phosphodiesterase type II (Wang and Young 1996), polyomavirus enhancer-binding protein 2 ␤ (Chen et al. 1999), nitrogen permease regulator 2 (Wang et al. 1999), aldolase A (Hua et al. 2000, and diphosphoinositol polyphosphate phosphohydrolase II (Hua et al. 2001). Unexpectedly, during the course of experiments amplifying the 5 Ј end of a putative lithium-regulated ddPCR fragment, we isolated another cDNA clone, a homolog of human/mouse transmembrane-4-superfamily (TM4SF) protein, CD151. The transcript levels of CD151 were significantly decreased in the rat frontal corte...

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Section: Discussionmentioning

confidence: 66%

Section: Pcr Amplification Of a Novel Cdna And Regulation By Mood Stamentioning

confidence: 99%

mentioning

confidence: 99%

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Tetraspan Protein CD151 A Common Target of Mood Stabilizing Drugs?

Hua

Green

Wong

et al. 2001

Neuropsychopharmacology

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“…The DIPPs are very active enzymes: four mammalian genes have been cloned, all of which encode proteins of around 20 kDa in size: type 1 Chu et al, 2004), types 2␣/2␤ Hua et al, 2001), and types 3␣/3␤ (Hidaka et al, 2002;Leslie et al, 2002;Hua et al, 2003). The specificity constants (k cat /K m ) for these enzymes range in value from 2 ϫ 10 5 to 5 ϫ 10 7 M Ϫ1 s Ϫ1 , the latter being close to the limit for diffusion-controlled encounter between enzyme and substrate (Fersht, 1985).…”

Section: Cellular Levels Metabolic Turnover and The Issue Of Comparmentioning

confidence: 99%

Diphosphoinositol Polyphosphates: Metabolic Messengers?

Shears¹

2009

Mol Pharmacol

138

148

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The diphosphoinositol polyphosphates ("inositol pyrophosphates") are a specialized subgroup of the inositol phosphate signaling family. This review proposes that many of the current data concerning the metabolic turnover and biological effects of the diphosphoinositol polyphosphates are linked by a common theme: these polyphosphates act as metabolic messengers. This review will also discuss the latest proposals concerning possible molecular mechanisms of action of this intriguing class of molecules.The discovery of cyclic AMP Sutherland and Rall, 1958) introduced us to the concept of a "second messenger" (Robison et al., 1968): a diffusible molecule (or ion) that, in response to an extracellular stimulus, is rapidly generated at (or released from) a particular subcellular site and then regulates particular effector proteins within the cell so as to elicit a cellular response. Of course, evolution has a remarkable tendency to repeat a good idea, so many different second messengers are now known. The inositol phosphate family represents the convergence of several "good signaling ideas," most notably in their use of a recurring theme in the cell-signaling genre: phosphate groups. Phosphates have two especially prominent features that facilitate specificity of interactions between cell signaling entities. First, the bulky nature of the phosphate group imposes geometric constraints on ligand-protein and protein-protein interactions. Second, the phosphate's negative charge at physiological pH bestows specificity on its interactions with target proteins through multiple ionic and hydrogen bonds. The negative charges on the phosphate group also make soluble, phosphorylated molecules lipid-impermeant, so that they can be retained inside cells.Inositol offers several additional assets for a signaling molecule. It is chemically stable, it is small (hence it diffuses through cytosol quickly), and it is only a short synthetic offshoot from the glycolytic pathway (Sherman et al., 1977). There is also a functionally significant plane of symmetry across the 2/5-axis of the inositol ring (Fig. 1). That symmetry permits one inositol phosphate to imitate another's threedimensional phosphate recognition pattern when the orientation of the inositol ring changes in relation to the protein's ligand-binding site (Wilcox et al., 1994), although in addition the binding site itself has to be somewhat flexible . This phenomenon provides a molecular explanation for the metabolic promiscuity of certain inositol phosphate kinases (for review, see Shears, 2004), and it also facilitates functionally important metabolic interactions between differ-

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“…As all these substrates are either potentially toxic, cell signaling molecules, or metabolic intermediates whose concentrations require modulation during the cell cycle, it has been postulated that the role of Nudix hydrolases is to sanitize or regulate the accumulation of these metabolites (4). To date, a number of Nudix hydrolases from different organisms have been characterized with respect to their major substrates such as nucleotide sugars (5), Ap n A (n ϭ 3, 4, 5, and 6) (6 -12), deoxynucleoside triphosphates (13)(14)(15)(16)(17), ADP-ribose (18 -23), GDP-mannose (5,25), NADH (26 -29), coenzyme A (30 -32), and diphosphoinositol polyphosphates (33)(34)(35).…”

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confidence: 99%

Cloning and Characterization of the First Member of the Nudix Family from Arabidopsis thaliana

Dobrzanska¹,

Szurmak²,

Wysłouch‐Cieszyńska³

et al. 2002

Journal of Biological Chemistry

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The sequence motif commonly called a Nudix box, represented by (GX 5 EX 7 REVXEEXGU) is the marker of a widely distributed family of enzymes that catalyze the hydrolysis of a variety of nucleoside diphosphate derivatives. Here we describe the cloning and characterization of an Arabidopsis thaliana cDNA encoding a Nudix hydrolase that degrades NADH. The deduced amino acid sequence of AtNUDT1 contains 147 amino acids. The recombinant AtNUDT1 was expressed in Escherichia coli and purified. In the presence of Mn 2؉ and the optimal pH of 7. 0, the recombinant AtNUDT1 catalyzed the hydrolysis of NADH with a K m value of 0. 36 mM. A V max of 12. 7 units mg ؊1 for NADH was determined. The recombinant AtNUDT1 migrated as a dimer on a gel filtration column. Biochemical analysis of recombinant AtNUDT1 indicated that the first characterized member of the Nudix family from A. thaliana is a NADH pyrophosphatase.

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Molecular Cloning of a Novel Isoform of Diphosphoinositol Polyphosphate Phosphohydrolase: A Potential Target of Lithium Therapy

Cited by 21 publications

References 43 publications

Tetraspan Protein CD151 A Common Target of Mood Stabilizing Drugs?

Tetraspan Protein CD151 A Common Target of Mood Stabilizing Drugs?

Diphosphoinositol Polyphosphates: Metabolic Messengers?

Cloning and Characterization of the First Member of the Nudix Family from Arabidopsis thaliana

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