Molecular cloning of a novel water channel from rice: its products expression in Xenopus oocytes and involvement in chilling tolerance

Li, Legong; Li, Shifang; Yan, Tang; Kitagawa, Yoshichika

doi:10.1016/s0168-9452(99)00269-1

Cited by 70 publications

(65 citation statements)

References 23 publications

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“…At the mRNA level, decreased expression of individual PIP genes during chilling was observed in rice [19,7], maize [6] and Arabidopsis [20]. We determined the expression of all the 11 PIP genes by real-time RT-PCR using TaqMan-MGB probes, and found that chilling reduced the expression of most PIP genes in our experiments (Figure 3), a result consistent with a previous report [7].…”

Section: Discussionsupporting

confidence: 91%

“…The composite water transport model proposes that water channels play a role in cell-tocell water flow under adverse environmental conditions when low transpiration and xylem tension occurs [17,18]. Aquaporins have previously been reported to be involved in chilling responses [4,19] but only recently have comprehensive analysis of aquaporin expression at the mRNA level in response to low temperature been done. Techniques used were Northern blot, semi-quantitative reverse transcription (RT)-PCR and real-time RT-PCR using SYBR green dye in species such as Arabidopsis [20], maize [6] and rice [7].…”

Section: Introductionmentioning

confidence: 99%

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Water relations and an expression analysis of plasma membrane intrinsic proteins in sensitive and tolerant rice during chilling and recovery

et al. 2006

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A symptom of chilling injury is development of water deficit in shoots, resulting from an imbalance of water transport and transpiration. In this work, two rice varieties (Oryza sativa L. var. Wasetoitsu and Somewake) seedlings were chilled at 7 o C, followed by recovery at 28 o C. Based on the growth phenotype and electrolyte leakage tests, Somewake was shown to be a chilling-tolerant variety, and Wasetoitsu a chilling-sensitive one. The chilling stress reduced markedly the relative water content (RWC) of leaves, accumulative transpiration and osmotic root hydraulic conductivity (Lp) in both varieties. But when returned to 28 o C, the water relation balance of Somewake recovered better. The mRNA expression profile of all the 11 plasma membrane intrinsic proteins (PIPs), a subgroup of aquaporins, was subsequently determined by real-time reverse transcription (RT)-PCR with TaqMan-minor grove binder (MGB) probes derived from rice var. Nipponbare during chilling treatment and recovery. Most of the PIP genes was down-regulated at the low temperature, and recovered at the warm temperature. The relative expression of some PIPs in both Somewake and Wasetoitsu decreased in parallel during the chilling. However during the recovery, the relative expression of OsPIP1;1, OsPIP2;1, OsPIP2;7 in shoots and OsPIP1;1, OsPIP2;1 in roots were significantly higher in Somewake than Wasetoitsu. This supports the role of PIPs in re-establishing water balance after chilling conditions. We discuss the diversified roles played by members of the aquaporin PIP subfamily in plant chilling tolerance depending on aquaporin isoforms, plant tissue and the stage of chilling duration.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Water relations and an expression analysis of plasma membrane intrinsic proteins in sensitive and tolerant rice during chilling and recovery

et al. 2006

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“…Different research groups reached different conclusions on the function of PIP proteins. It has previously been reported that OsPIP1-1 and OsPIP1-3 had ability to transport water in the Xenopus oocytes [30,32], but other studies showed that only PIP2 proteins showed a rapid water transport activity, whereas PIP1s had no or a low water transport activity in the Xenopus oocytes [28,39]. Our data indicated that the transgenic plants over-expressing OsPIP1-1 or Os-PIP2-2 showed high tolerance to mild salt stress ( Figure 6C and 6D), suggesting that OsPIP genes play important roles in regulating water homeostasis, and that both OsPIP1-1 and OsPIP2-2 could possibly have intrinsic water transport ability in planta, which needs to be further confirmed biochemically in vivo.…”

Section: Discussionmentioning

confidence: 96%

“…OsPIP1-1, npg 290 amino acid residues, and the sequence similarity among them ranged from 57% (between OsPIP1-3 and OsPIP2-7) to 92% (between OsPIP1-1 and OsPIP1-2). The 10 OsPIP proteins were found sharing six conserved transmembrane domains (TMs) and five connecting loops ( Figure 1A) [30,36,37]. Moreover, the sequences around the NPA motifs (Asn-Pro-Ala) were highly conserved ( Figure 1A).…”

Section: Identification and Sequence Analysis Of Ospips In Ricementioning

confidence: 99%

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Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family

et al. 2006

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Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporins that enable fast and controlled translocation of water across the membrane. In this study, we systematically identified and cloned ten PIP genes from rice. Based on the similarity of the amino acid sequences they encoded, these rice PIP genes were classified into two groups and designated as OsPIP1-1 to OsPIP1-3 and OsPIP2-1 to OsPIP2-7 following the nomenclature of PIP genes in maize. Quantitative RT-PCR analysis identified three root-specific and one leaf-specific OsPIP genes. Furthermore, the expression profile of each OsPIP gene in response to salt, drought and ABA treatment was examined in detail. Analysis on transgenic plants over-expressing of either OsPIP1 (OsPIP1-1) or OsPIP2 (OsPIP2-2) in wild-type Arabidopsis, showed enhanced tolerance to salt (100 mM of NaCl) and drought (200 mM of mannitol), but not to salt treatment of higher concentration (150 mM of NaCl). Taken together, these data suggest a distinct role of each OsPIP gene in response to different stresses, and should add a new layer to the understanding of the physiological function of rice PIP genes.

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Metalloid Transport Systems

Jiang

Rosen

2010

Biological Chemistry of Arsenic, Antimony and Bismuth

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Molecular cloning of a novel water channel from rice: its products expression in Xenopus oocytes and involvement in chilling tolerance

Cited by 70 publications

References 23 publications

Water relations and an expression analysis of plasma membrane intrinsic proteins in sensitive and tolerant rice during chilling and recovery

Water relations and an expression analysis of plasma membrane intrinsic proteins in sensitive and tolerant rice during chilling and recovery

Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family

Metalloid Transport Systems

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