Molecular Cloning of a Widely Distributed Microsatellite Core Sequence from the Cultivated Mushroom Agaricus bisporus

Barroso, Gérard; Sonnenberg, A.S.M.; Griensven, L. J. L. D. van; Labarère, Jacques

doi:10.1006/fgbi.2000.1239

Cited by 15 publications

(8 citation statements)

References 36 publications

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“…Each VNTR includes a short, highly conserved region, known as a core sequence (Jeffreys et al, 1985), that can be used as a single primer in PCR to direct the amplification to regions rich in minisatellite repeats, a technique denominated Direct Amplification of Minisatellite‐region DNA (DAMD) (Heath et al, 1993). This methodology has been used in some fish, birds (Heath et al, 1993), rice (Zhou et al, 1997), wheat (Somers et al, 1996; Bebeli et al, 1997), bean (Metais et al, 2000), and fungal species (Barroso et al, 2000; Santini and Capreti, 2000) to detect DNA polymorphism through RAPD‐like results. As these VNTR core sequences are longer than RAPD primers, this methodology can also be effectively carried out at relatively high stringencies (high PCR annealing temperatures), thus yielding more reproducible results.…”

Section: Introductionmentioning

confidence: 99%

PCR primed with minisatellite core sequences yields species-specific patterns and assessment of population variability in fishes of the genus Brycon

Wasko

Galetti

2003

Journal of Applied Ichthyology

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Summary Minisatellite core sequences were used as single primers in polymerase chain reaction (PCR) to amplify genomic DNA in a way similar to the random amplified polymorphic DNA methodology. This technique, known as Directed Amplification of Minisatellite‐region DNA, was applied in order to differentiate three neotropical fish species (Brycon orbignyanus, B. microlepis and B. lundii) and to detect possible genetic variations among samples of the threatened species, B. lundii, collected in two regions with distinct environmental conditions in the area of influence of a hydroelectric dam. Most primers generated species‐specific banding patterns and high levels of intraspecific polymorphism. The genetic variation observed between the two sampling regions of B. lundii was also high enough to suggest the presence of distinct stocks of this species along the same river basin. The results demonstrated that minisatellite core sequences are potentially useful as single primers in PCR to assist in species and population identification. The observed genetic stock differentiation in B. lundii associated with ecological and demographic data constitute a crucial task to develop efficient conservation strategies in order to preserve the genetic diversity of this endangered fish species.

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Section: Introductionmentioning

confidence: 99%

PCR primed with minisatellite core sequences yields species-specific patterns and assessment of population variability in fishes of the genus Brycon

Wasko

Galetti

2003

Journal of Applied Ichthyology

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“…The number of repeat units in a particular microsatellite region generates DNA polymorphism which can be detected by specific polymerase chain reaction (PCR) primers amplifying such regions (37). Microsatellites have become powerful tools in genetic analyses of numerous organisms, including animals, plants, fungi, and humans, for the study of genomic evolution, population structure, gene flow, intraspecific phylogeny, mating systems, and genetic mapping (2,8,37). Recently, highly polymorphic microsatellite markers were developed for studying population genetics of rice-, soybean-, and maize-infecting R. solani AG-1 IA (3,46) and potato-infecting R. solani AG-3 (12).…”

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confidence: 99%

Population Structure ofRhizoctonia oryzae-sativaein California Rice Fields

Chaijuckam¹,

Baek²,

Greer³

et al. 2010

Phytopathology®

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Six pairs of single-locus microsatellite primers were developed to study the population structure of Rhizoctonia oryzae-sativae, the cause of aggregate sheath spot disease of rice, among and within three rice-growing areas in California over a 3-year period. A high level of gene flow among growing areas was indicated by low population subdivision according to analysis of molecular variance and moderate to no population differentiation between pairs of populations based on the fixation index (F(ST)). Gametic equilibrium of most pairs of microsatellite loci, high numbers of unique multilocus genotypes, and high genotypic diversity indicated extensive sexual recombination within growing areas. Because there was little differentiation among populations in all hierarchical levels, including among growing areas within sampling years, fields within growing areas, and corners within individual fields, a high level of gene flow was revealed in all levels. Basidiospores were likely the main vehicle of gene flow among populations, including short and long distances. Asexual inocula (sclerotia and mycelia) probably overwinter because a few clones were detected over a 2-year period within the same field. A few clones were shared among fields but were not commonly shared among growing areas.

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“…This makes strain protection problematic, and impedes strain improvement. Molecular markers of rDNA sequencing, RFLP (restriction fragment length polymorphism), RAPD (random amplified polymorphic DNA), microsatellite and mitochondrial genotypes have all been used to discriminate mushroom species and/or strains of Agaricus (Castle et al 1987;Sonnenberg et al 1991;Khush et al 1992;Barroso et al 2000;Calvo-Bado et al 2000;Ramirez et al 2001), Auricularia (Yan et al 1999), Ganoderma (Hseu et al 1996), Lentinula (Chiu et al 1996), Stropharia rugoso-annulata (Yan et al 2003), and Volvariella (Chiu et al 1995). These technologies provide ways to obtain reliable data for mushroom strain identification and protection.…”

Section: Introductionmentioning

confidence: 99%

RAPD molecular differentiation of the cultivated strains of the jelly mushrooms, Auricularia auricula and A. polytricha

Yan¹,

Luo

Zhou

2004

World J Microbiol Biotechnol

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Auricularia mushrooms are the fourth most important cultivated mushrooms in the world, with a unique jelly taste and horizontal-septated basidium which are significantly different from other cultivated mushrooms. Differentiation of commercial cultivated strains is difficult to conduct, due to the lack of useful distinguishable characters. In this study, we used the RAPD technique to differentiate 11 commercial strains of A. auricula and five commercial strains of A. polytricha and one white-fruitbody mutant strain, and to characterize their genetic diversity. Results showed that all the strains tested could be differentiated by pooled RAPD data, and even one individual primer (S10) could also discriminate all tested strains. RAPD analysis could differentiate strains having identical rDNA RFLP and supports the classification of the white-fruitbody mutant to the species of A. polytricha. Genetic similarity analysis and grouping derived from RAPD markers reveals a high level of genetic diversity of commercial strains of Auricularia auricula and A. polytricha. Therefore, the RAPD technique can provide a powerful tool to discriminate the commercial Auricularia strains and offer the molecular information useful for breeding systems.

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Molecular Cloning of a Widely Distributed Microsatellite Core Sequence from the Cultivated Mushroom Agaricus bisporus

Cited by 15 publications

References 36 publications

PCR primed with minisatellite core sequences yields species-specific patterns and assessment of population variability in fishes of the genus Brycon

PCR primed with minisatellite core sequences yields species-specific patterns and assessment of population variability in fishes of the genus Brycon

Population Structure ofRhizoctonia oryzae-sativaein California Rice Fields

RAPD molecular differentiation of the cultivated strains of the jelly mushrooms, Auricularia auricula and A. polytricha

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