Molecular Cloning of Bottle-Nosed Dolphin (Tursiops truncatus) MHC Class I cDNA.

Shirai, Katsuaki; Sakai, Takeo; Oike, Tatsuya

doi:10.1292/jvms.60.1093

Cited by 16 publications

(14 citation statements)

References 16 publications

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“…Currently, studies on the genetic variation of MHC class I genes in cetaceans are limited, although some studies have been reported for the fin whale (Balaenoptera physalus), the sei whale (Balaenoptera borealis), the gray whale (Eschrichtius robustus), the finless porpoise (Neophocaena phocaenoides), and the North Atlantic right whale (Eubalaena glacialis) (Flores-Ramirez et al 2000, 2004Gillett et al 2014;Trowsdale et al 1989;Xu et al 2007). In addition, cDNA amplification has been used to confirm the expression of MHC class I genes in the bottlenose dolphin (Tursiops truncatus) and Hector's dolphin (Cephalorhynchus hectori) (Heimeier et al 2009;Shirai et al 1998). Most studies suggest the presence of at least three MHC class I loci in cetaceans.…”

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Assembly and characterization of the MHC class I region of the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis)

et al. 2015

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The Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis; YFP) is the sole freshwater subspecies of N. asiaeorientalis and is now critically endangered. Major histocompatibility complex (MHC) is a family of highly polymorphic genes that play an important immunological role in antigen presentation in the vertebrates. Currently, however, little is known about MHC region in the genome of the YFP, which hampers conservation genetics and evolutionary ecology study using MHC genes. In this work, a nucleotide sequence of 774,811 bp covering the YFP MHC class I region was obtained by screening a YFP bacterial artificial chromosome (BAC) library, followed by sequencing and assembly of positive BAC clones. A total of 45 genes were successfully annotated, of which four were MHC class I genes. There are high similarities among the four YFP MHC class I genes (>94 %). Divergence in the coding region of the four YFP MHC class I genes is mainly localized to exons 2 and 3, which encode the antigen-binding sites of MHC class I genes. Additionally, comparison of the MHC structure in YFP to those of cattle, sheep, and pig showed that MHC class I genes are located in genome regions with regard to the conserved genes, and the YFP contains the fewest MHC class I genes among these species. This is the first report characterizing a cetacean MHC class I region and describing its organization, which would be valuable for further investigation of adaptation in natural populations of the YFP and other cetaceans.

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Assembly and characterization of the MHC class I region of the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis)

et al. 2015

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“…Since a whaling moratorium in 1945, the population appears to have recovered from an estimated 3000 to approximately 20,000 individuals ( Buckland et al 1993;Henderson 1984). Aspects of gray whale behavior and (Shirai et al 1998) MHC-I (a) exon 2 and (b) exon 3 sequences with mouse ( H2-K b , U47328), ( H2-K d , U47329); leopard ( LPAI69J, U07676); cheetah (AJUI1, U07665); harbor seal (PLA-A1, U88874); human ( HLA-B-0701, U21052); cattle ( BoLA, AB008626); and swine (SLA-I, AF074426). Sequences were aligned using the Clustal-W program ( Thompson et al 1994).…”

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Major histocompatibility complex class I loci from the gray whale (Eschrichtius robustus)

Flores-Ramírez

Urbán‐Ramírez²,

Miller³

2000

Journal of Heredity

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Sequences from exons encoding the peptide binding region of MHC class I (MHC-I) molecules were isolated from California gray whale (Eschrichtius robustus) genomic DNA to initiate an investigation of variation in these genes in a cetacean. These represent the first mysticete MHC-I sequences to be reported. The analysis of gray whale MHC-I sequences suggests the presence of at least three loci, which share greatest similarity to MHC-I in the ungulates, consistent with current views on cetacean phylogenetics. The peptide binding region of MHC is the most polymorphic part of the molecule and analysis of the variation and synonymous to nonsynonymous substitution ratios in gray whale sequences found these genes to display polymorphism characteristics similar to that attributed to selection in other species.

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“…5). The bottlenose dolphin sequence shown in the alignment (see Supporting Figure S4) has not been deposited in GenBank and was entered directly from the published article (Shirai et al . 1998).…”

Section: Resultsmentioning

confidence: 99%

“…2000, 2004), the finless porpoise ( Neophocaena phocaenoides ) (Xu et al . 2007), the vaquita ( Phocoena sinus ) (Munguia‐Vega 2003), and in the bottlenose dolphin ( Tursiops truncates ) (Shirai et al . 1998).…”

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Confirmed expression of MHC class I and class II genes in the New Zealand endemic Hector's dolphin (Cephalorhynchus hectori)

Heimeier¹,

Baker²,

Russell³

et al. 2009

Marine Mammal Science

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We provide the first description and confirmation of expression for three Major Histocompatibility Complex (MHC) genes (DQA, DQB, and class I) in the New Zealand endemic Hector's dolphin (Cephalorhynchus hectori). Sequences were derived from cDNA generated from peripheral blood leukocyte mRNA collected during a temporary live capture of two Hector's dolphins. The complete exons 2 and 3 of DQA, DQB, and class I were amplified from cDNA to confirm their expression. The same primers proved reliable for amplification from genomic DNA. Locus identity was supported by phylogenetic relationships to MHC molecules from other closely related species. No evidence was found for duplication of DQ loci, but cloning of amplified exon 2 of the class I gene indicated duplication or triplication of this locus. However, the expression of multiple class I loci was uncertain as only one allele could be detected from clones of cDNA. Balancing selection was indicated by a significantly greater ratio of nonsynonymous to synonymous substitutions at all three loci and by the apparent transspecific sharing of alleles for DQA and DQB.

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Molecular Cloning of Bottle-Nosed Dolphin (Tursiops truncatus) MHC Class I cDNA.

Cited by 16 publications

References 16 publications

Assembly and characterization of the MHC class I region of the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis)

Assembly and characterization of the MHC class I region of the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis)

Major histocompatibility complex class I loci from the gray whale (Eschrichtius robustus)

Confirmed expression of MHC class I and class II genes in the New Zealand endemic Hector's dolphin (Cephalorhynchus hectori)

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