Molecular cloning of cDNA encoding MS2 antigen, a novel cell surface antigen strongly expressed in murine monocytic lineage

Abstract

cDNA clones which strongly hybridized with a 3.1 kb mRNA from mouse macrophages and macrophage cell lines and weakly with mRNA from P815 but not from a variety of other cell lines and tissues were isolated from cDNA libraries constructed using mRNA from murine macrophage cell lines and peritoneal macrophages. Treatment of a macrophage cell line with macrophage stimulators significantly enhanced transcription of the mRNA. Sequencing analysis of these clones demonstrated that the cDNA consisted of 3036 bp insert containing a 2478 bp open reading frame followed by a 538 bp 3' untranslated region. The amino acid sequence, deduced from the nucleotide sequence of the cDNA, predicted a protein containing a signal peptide, an extracellular region, a transmembrane domain, and a cytoplasmic tail. The extracellular region had five putative N-glycosylation sites and a cysteine-rich domain, whereas the cytoplasmic region consisted of a proline-rich amino acid sequence significantly similar to CD2. SDS-PAGE and NEPHGE SDS-PAGE analysis of the immunoprecipitated membrane of the macrophage cell lines prepared by using rabbit anti-MS2 peptide antibody raised against a synthetic peptide preparation relative to a hydrophilic region of the MS2 amino acid sequence confirmed that MS2 protein is a cross-linked protein having approximate molecular sizes of 89 kd and pl 6.5-7.0. These results show that MS2 protein is a novel cell surface antigen expressed mainly in monocytic lineages.

PrPCHomodimerization Stimulates the Production of PrPCCleaved Fragments PrPN1 and PrPC1

“…5A). Amplification of ADAM8 was verified in the human monocytic cell line U-937 (Yoshida et al, 1990). To assess the role of ADAM10 and ADAM17, we used the metalloproteinase inhibitor marimastat.…”

Cloning and characterization of ADAM28: evidence for autocatalytic pro-domain removal and for cell surface localization of mature ADAM28

“…Little is known about the activity or functions of the other 13 ADAMs with a catalytic site consensus sequence, except that seven of them appear to be expressed predominantly in the testis, suggesting a role in sperm function [4,[15][16][17][18][19][20][21][22][23]. ADAMs harbouring a catalytic site that are known to be expressed in somatic cells include MDC9 [24], ADAM10\KUZ [8,9,25], ADAM8\ MS2 [26], ADAM12\meltrin α [27], MDC15 [28][29][30], ADAM17\ TACE (tumour necrosis factor converting enzyme) [10,11] and ADAM19\meltrin β [31,32]. Here we report the cDNA cloning and initial biochemical characterization of the mouse protein ADAM28, a catalytic-site-containing ADAM that is expressed in at least two somatic tissues, mouse epididymis and lung.…”

Structure of the Murine CD156 Gene, Characterization of Its Promoter, and Chromosomal Location

“…The mouse CD156 (mCD156) 1 (MS2) and human CD156 are type I transmembrane glycoproteins found on myelomonocytic cell lineage (1,2). The mCD156 cDNA is 3.2 kb long with an open reading frame encoding 826 amino acids.…”