Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens.

Szala, Stanisław; Kasai, Yasushi; Steplewski, Zenon; Rodeck, Ulrich; Koprowski, Hilary; Linnenbach, Alban J.

doi:10.1073/pnas.87.17.6833

Cited by 131 publications

(91 citation statements)

References 30 publications

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“…A15 (Talla-1) was identified as a highly specific surface marker of T-cell acute lymphoblastic leukemia and neuroblastoma, 20,21 whereas CO-029 is frequently highly expressed in stomach, colon and pancreas carcinoma. 22 Our study provides evidence that NET-1/C4.8 represents another member of the tetraspanin family of proteins, whose upregulation is clearly associated with carcinogenesis. The potential use of this gene as a prognostic marker for cervical cancer will have to be evaluated in further studies.…”

Section: Discussionmentioning

confidence: 88%

Identification of a new proliferation‐associated protein NET‐1/C4.8 characteristic for a subset of high‐grade cervical intraepithelial neoplasia and cervical carcinomas

Wollscheid

Kühne-Heid

Stein

et al. 2002

Intl Journal of Cancer

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A large number of genes are known to be differentially expressed at distinct steps of carcinogenesis. By using a cell culture model system for cervical cancer, we had previously identified several genes that were more strongly expressed when comparing normal cervical epithelium with cervical intraepithelial neoplasia (CIN) and cervical cancer. In our study, we show that one of these genes, C4.8, is identical to NET-1, which is a new member of the tetraspanin family of proteins. By generating a mouse polyclonal antiserum against the major extracellular domain of the protein, we could detect NET-1/C4.8 expression both after ectopic expression of the gene in cell cultures and in cryostat sections of cervical biopsies. Moreover, immunohistochemic analyses of normal cervical epithelium, metaplasia, condyloma and CIN of different severity suggest that NET-1/C4.8 expression is associated with neoplastic cell proliferation. Notably, expression of the protein throughout the entire epithelium is only evident for a subset of CIN3. The potential importance for this gene in cervical carcinogenesis is underlined by an invariably strong expression in all undifferentiated squamous cell cancers examined. This indicates that this gene may be of prognostic value.

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Section: Discussionmentioning

confidence: 88%

Identification of a new proliferation‐associated protein NET‐1/C4.8 characteristic for a subset of high‐grade cervical intraepithelial neoplasia and cervical carcinomas

Wollscheid

Kühne-Heid

Stein

et al. 2002

Intl Journal of Cancer

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“…This cluster included the epithelial membrane protein EMP1, the connective tissue genes COL14A1, decorin (DCN), elastin (ELN), and the putative elastin associated gene MFAP4 (Zhao et al, 1995). Interestingly, this cluster also included two transmembrane four superfamily proteins, TM4SF1 and TM4SF3, which have been found to be expressed in lung, breast, ovarian, gastric, colon, rectal, and pancreatic tumors, but not in most normal tissues (Szala et al, 1990;Marken et al, 1992). A second cluster of genes that were predictive of T histology were downregulated in T samples and included genes that are involved in cell cycle and proliferation, such as CENPA, CDCA1, CCNB1, CDC20, and MCM5.…”

Section: Differentiation Programs From Classifier Genesmentioning

confidence: 99%

Gene expression-based classification of nonseminomatous male germ cell tumors

et al. 2005

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Male adult germ cell tumors (GCTs) comprise two major histologic groups: seminomas and nonseminomas. Nonseminomatous GCTs (NSGCTs) can be further divided into embryonal carcinoma (EC), teratoma (T), yolk sac tumor (YS), and choriocarcinoma (CC) on the basis of the lineage differentiation that they exhibit. NSGCTs frequently present as mixed tumors consisting of two or more histological subtypes, often limiting correlative studies of clinical and molecular features to histology. We sought to develop a molecular classifier that could predict the predominant histologic subtype within mixed NSGCT tumor samples. The expression profiles of 84 NSGCTs (42 pure and 42 mixed) and normal age-matched testes were obtained using Affymetrix microarrays. Using prediction analysis for microarrays, we identified 146 transcripts that classified the histology of pure NSGCTs samples with 93% accuracy. When applied to mixed NSGCTs, the classifier predicted a histology that was consistent with one of the reported components in 93% of cases. Among the predictive transcripts were CGB (high in CC), LCN2 (high in T), BMP2 (high in YS), and POU5F1 (high in EC). Thus, the expression-based classifier accurately assigned a single predominant histology to mixed NSGCTs, and identified transcripts differentially expressed between histologic components with relevance to NSGCT differentiation.

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“…The ®rst one has been shown to be a variant isoform of the adhesion molecule CD44, CD44v4-v7 (GuÈ nthert et al, 1991). D6.1A is a tetraspanin molecule and the ortholog of the human tumor antigen CO-029 (Szala et al, 1990a). Likely by association with integrins or additional membrane structures the molecule can initiate activation of the clotting cascade .…”

Section: Introductionmentioning

confidence: 99%

Metastasis-association of the rat ortholog of the human epithelial glycoprotein antigen EGP314

Würfel

Rösel

Seiter³

et al. 1999

Oncogene

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Screening for surface molecules expressed by metastasizing rat tumors had revealed evidence for metastasisassociation of a molecule also expressed on epithelial cells. The similarity to the expression pro®le of the panepithelial glycoprotein EGP314 prompted us to isolate and sequence the gene and to explore functional features of the molecule in transfected tumor lines. The molecule D5.7A, named according to the antibody, D5.7, used for selection, indeed, is the ortholog of EGP314 with 92% and 80% identity to the murine and the human molecules. Like EGP314, D5.7A has a particular cleavage site, a small cleavage product being resolved under reducing conditions from the membrane anchored part of the molecule. Transfection of a low metastasizing ®brosarcoma, pheochromoblastoma and adenocarcinoma revealed that expression of D5.7A facilitates tumor progression. Depending on the origin of the tumor, D5.7A transfectants either metastasized via the lymphatic system (pheochromoblastoma, adenocarcinoma) or hematogeneously (®brosarcoma). Particularly after proteolytic cleavage, D5.7A facilitated cell ± cell adhesion and provided a proliferative signal upon crosslinking. Thus, the rat ortholog of EGP314 is involved in metastasis formation. Importantly, its functional activities apparently rely on proteolytic cleavage. These ®ndings provide a ®rst evidence on how a panepithelial marker can be involved in tumor progression.

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Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens.

Cited by 131 publications

References 30 publications

Identification of a new proliferation‐associated protein NET‐1/C4.8 characteristic for a subset of high‐grade cervical intraepithelial neoplasia and cervical carcinomas

Identification of a new proliferation‐associated protein NET‐1/C4.8 characteristic for a subset of high‐grade cervical intraepithelial neoplasia and cervical carcinomas

Gene expression-based classification of nonseminomatous male germ cell tumors

Metastasis-association of the rat ortholog of the human epithelial glycoprotein antigen EGP314

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