Molecular cloning of feline Fas antigen and Fas ligand cDNAs

Mizuno, Takuya; Endo, Yasuyuki; Momoi, Yasuyuki; Goto, Yuko; Nishimura, Yoshiaki; Tsubota, Kenjiro; Mikami, Taisei; Ohno, Koichi; Watari, Toshihiro; Tsujimoto, Hajime; Hasegawa, Atsuhiko

doi:10.1016/s0165-2427(98)00151-2

Cited by 10 publications

(13 citation statements)

References 45 publications

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“…(2)(3)(4) To date, rat, mouse, human, rhesus macaque, and feline cDNA encoding FasL have been isolated. (4)(5)(6)(7)(8) FasL is a type II membrane-type cytokine that belongs to the tumor necrosis factor (TNF) family. (4) It is expressed mainly in activated T cells, whereas Fas antigen is broadly expressed in various tissues.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning, Characterization, and Expression of Porcine Fas Ligand (CD95 Ligand)

Muneta

Shimoji²,

Inumaru³

et al. 2001

Journal of Interferon & Cytokine Research

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We isolated and sequenced cDNA that contained the coding sequence of porcine Fas ligand (FasL). Using mixed oligonucleotide primers based on the 5' and 3' nucleotide sequences conserved among human, murine, and rat FasL, we performed the reverse transcription polymerase chain reaction (RT-PCR) with total RNA prepared from porcine thymocytes stimulated with 5 microg/ml concanavalin A (ConA) to clone the cDNA of porcine FasL. The open reading frame (ORF) of porcine FasL cDNA was 849 base pairs (bp) in length and encoded 282 amino acids. The predicted amino acid sequence was 85.5%, 76.6%, and 75.5% homologous to the predicted human, murine, and rat FasL, respectively. The recombinant porcine FasL expressed by recombinant baculovirus containing the whole coding sequences of porcine FasL showed cytotoxic effect and induced apoptosis in porcine renal tubular cell line PK-15 cells sensitized by cycloheximide (CHX), which was confirmed by MTT assay, DNA fragmentation assay, and TUNEL staining, respectively. Furthermore, the mRNA expression of porcine FasL in porcine peripheral blood lymphocytes (PBL) was induced by porcine interleukin-18 (IL-18). These results indicate that porcine FasL identified in this study is biologically functional and has the ability to induce apoptosis as reported in other species.

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Section: Introductionmentioning

confidence: 99%

Molecular Cloning, Characterization, and Expression of Porcine Fas Ligand (CD95 Ligand)

Muneta

Shimoji²,

Inumaru³

et al. 2001

Journal of Interferon & Cytokine Research

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show abstract

“…Fluorescence in situ hybridization (FISH) was performed essentially as described previously (Satoh et al, 1993). A genomic clone of the feline FasL gene (FGFasL) was isolated from a cat PBMC genomic library constructed with Lambda FIX-II vector (Stratagene, CA) by screening with a feline FasL cDNA clone (pCRFL) (Mizuno et al, 1998) as a probe. The FGFasL clone contained F11-kb insert of feline genomic FasL and was used for FISH.…”

Section: Methodsmentioning

confidence: 99%

“…Based on the nomenclature for feline Q-banded chromosomes (Cho et al, 1997), the chromosome locus was determined. (Mizuno et al, 1998) Gene reference: The nucleotide sequence of feline FasL cDNA was published in Mizuno et al (1998). …”

Section: Methodsmentioning

confidence: 99%

Assignment<footref rid="foot01"><sup>1</sup></footref> of the feline Fas ligand gene (TNFSF6) to chromosome F1q12→q13 by fluorescence in situ hybridization

Fujino

Mizuno

Masuda

et al. 2001

Cytogenet Genome Res

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“…FISH was performed essentially as described previously (Satoh et al, 1993;Fujino et al, 2001). A genomic clone of the feline Fas gene (FGFasA) was isolated from a cat PBMC genomic library constructed with Lambda FIX-II vector (Stratagene, CA) by screening with a feline Fas cDNA clone (pCRFA) (Mizuno et al, 1998) as a probe. The FGFasA clone contained an F20-kb insert of feline genomic Fas and was used for FISH.…”

Section: Methodsmentioning

confidence: 99%

“…The FGFasA clone contained an F20-kb insert of feline genomic Fas and was used for FISH. The chromosome locus was determined based on the nomenclature for feline Qbanded chromosomes (Cho et al, 1997 (Mizuno et al, 1998) Gene reference: the nucleotide sequence of the feline Fas cDNA was published in Mizuno et al (1998). …”

Section: Methodsmentioning

confidence: 99%

Assignment<footref rid="foot01"><sup>1</sup></footref> of the feline Fas (TNFRSF6) gene to chromosome D2p13→p12.2 by fluorescence in situ hybridization

Fujino

Mizuno

Masuda

et al. 2001

Cytogenet Genome Res

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Molecular cloning of feline Fas antigen and Fas ligand cDNAs

Cited by 10 publications

References 45 publications

Molecular Cloning, Characterization, and Expression of Porcine Fas Ligand (CD95 Ligand)

Molecular Cloning, Characterization, and Expression of Porcine Fas Ligand (CD95 Ligand)

Assignment<footref rid="foot01"><sup>1</sup></footref> of the feline Fas ligand gene (TNFSF6) to chromosome F1q12→q13 by fluorescence in situ hybridization

Assignment<footref rid="foot01"><sup>1</sup></footref> of the feline Fas (TNFRSF6) gene to chromosome D2p13→p12.2 by fluorescence in situ hybridization

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