Molecular cloning of FSH and LH β subunits and their regulation by estrogen in Atlantic croaker

Banerjee, Ananyo; Khan, Izhar Ahmad

doi:10.1016/j.ygcen.2007.09.016

Cited by 24 publications

(15 citation statements)

References 48 publications

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“…Sex steroid, either androgens or oestrogens, feedback on gonadotropin synthesis and release is well established in fish (Dickey & Swanson 1998, Kobayashi et al 2001, Huggard-Nelson et al 2002, Banerjee & Khan 2008. In zebrafish, information on oestrogen effects on gonadotropin levels (either subunits mRNA expression levels or plasma levels) is scarce.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have reported detrimental effects of oestrogenic compounds on fertility at all levels of the brainpituitary-gonad axis in teleost fish (Tsai et al 2005, Filby et al 2006, Zhang et al 2008, including impairment of spermatogenesis (Van der Ven et al 2003, Van den Belt et al 2004, Pawlowski et al 2004, and inhibition of androgen synthesis, either directly on Leydig cells (Loomis & Thomas 2000, Govoroun et al 2001, Baron et al 2005 or via feedback on hypothalamus and pituitary to control synthesis and release of gonadotropins (Dickey & Swanson 1998, Kobayashi et al 2001, Huggard-Nelson et al 2002, Banerjee & Khan 2008. However, it is not known exactly which step(s) in the developmental sequence constituting spermatogenesis is/are affected by oestrogens in fish.…”

Section: Introductionmentioning

confidence: 99%

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Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis

Waal¹,

Leal²,

García-López³

et al. 2009

Journal of Endocrinology

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Androgens can induce complete spermatogenesis in immature or prepubertal teleost fish. However, many aspects of the role of androgens in adult teleost spermatogenesis have remained elusive. Since oestrogens inhibit androgen synthesis, we used an oestrogen-induced androgen depletion model to identify androgen-dependent stages during adult zebrafish spermatogenesis. Exposure to 10 nM 17b-oestradiol (E 2 ) in vivo at least halved the mass of differentiating germ cells (from type B spermatogonia to spermatids), while type A spermatogonia accumulated. Studies on the cellular dynamics revealed that a reduction of spermatogonial proliferation together with an inhibition of their differentiation to type B spermatogonia were the basis for the oestrogen-mediated disturbance of spermatogenesis. The capacity of the zebrafish testis to produce 11-ketotestosterone as well as the expression of steroidogenesis-related genes was markedly decreased after in vivo oestrogen exposure. Moreover, the androgen-release response to recombinant zebrafish Lh was lost after oestrogen exposure. We conclude that oestrogen exposure caused a state of androgen insufficiency in adult male zebrafish. Since the downregulation of the steroidogenic system as well as the disturbance of spermatogenesis in testicular explants exposed to E 2 ex vivo was much less severe than after in vivo exposure, the main inhibitory effect appears to be exerted via feedback inhibition of gonadotropin release. This experimental set-up helped to identify spermatogonial proliferation and their differentiation as androgen targets in adult zebrafish spermatogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis

Waal¹,

Leal²,

García-López³

et al. 2009

Journal of Endocrinology

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show abstract

“…FSH and LH are complex heterodimer glycoproteins, composed of a common a-subunit and a hormone specific beta subunit (Li and Ford 1998). Since two chemically distinct GTHs were identified in chum salmon (Oncorhynchus keta) (Suzuki et al 1988a; Kawauchi et al 1989), the properties, structural and expression analyses of FSHb, LHb and common a-subunit have been reported in a number of teleost species (Banerjee and Khan 2007;Chang et al 1988Chang et al ,1990Elizur et al 1996;Hassin et al 1995;Jackson et al 1999;Jeng et al 2007;Kagawa et al1998;Kajimura et al 2000;Kim et al 2007;Li et al 2005;So et al 2005;Van Der Kraak et al 1992, Yoshiura et al 1997.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, characterization and expression of FSH and LH beta subunits from grass carp (Ctenopharyngodon idella)

Zhou

Niu

Tao

et al. 2008

Fish Physiol Biochem

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Follicle-stimulating hormone beta subunit (FSHbeta) and luteinizing hormone beta subunit (LHbeta) have been cloned and characterized from the pituitary of grass carp (Ctenopharyngodon idella). The full length of FSHbeta and LHbeta cDNA was 393 bp and 441 bp, with open reading frame encoding proteins of 130 and 146 amino acids, respectively. Phylogenetic analysis of the proteins of FSHbeta and LHbeta showed a high homology with other fishes. Homology analysis also indicated that LHbeta has higher conservation than FSHbeta. The expression analysis of grass carp FSHbeta and LHbeta by RT-PCR suggested that they were only expressed in the pituitary. Real-time quantitative PCR protocols were developed and validated to measure FSHbeta and LHbeta mRNAs during ovarian development. The FSHbeta and LHbeta mRNA level was very low in the pituitaries of early-pubertal fish and significantly increased during the ovulation period. These results suggested that in grass carp the gonadotropins synthesized synchronously in order for asynchronous oogenesis to take place.

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“…It has been well established that FSH plays an important role in gonadal development and gametogenesis in vertebrates, including fish (Banerjee & Khan 2008;Pham et al 2008). Recently, a vertebrate FSH-like substance has also been identified in Portunidae, the mud crab Scylla paramamosain and the swimming crab Portunas trituberculatus by immunological techniques (Huang et al 2008Ye et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In the Atlantic croaker (Micropogonias undulates Linnaeus, 1766) the FSH mRNA abundance was higher in the pituitaries of early pubertal than in late-maturing/mature individuals (Banerjee & Khan 2008). In tilapia (Oreochromis niloticus Linnaeus, 1758), the plasma level of FSH increased significantly during the second day after the eggs had been removed from the females, probably due to recruitment of a new generation of follicles for the successive spawning (Aizen et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of follicle-stimulating hormone-like substance in the Kuruma prawn,Marsupenaeus japonicus, during ovarian maturation

Ye¹,

Ma²,

Lin³

et al. 2011

Marine Biology Research

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The identification of vertebrate follicle-stimulating hormone (FSH) may further elucidate the reproductive mechanisms in the prawn Marsupenaeus japonicus. By immunohistochemistry, FSH-like neurons were detected in the brain and thoracic ganglia of the female kuruma prawn. By enzyme-linked immunosorbent assay (ELISA), concentrations of an FSH-like substance were determined in the brain, thoracic ganglia and haemolymph during the maturation of the ovaries. Peak levels of an FSH-like substance culminated in the brain and thoracic ganglia during the exogenous vitellogenic stage, while the maximum level of the FSH-like substance was detected in the haemolymph. Our results indicated that the vertebrate FSHlike substances are present in M. japonicus, suggesting they may be involved in the ovarian maturation.

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Molecular cloning of FSH and LH β subunits and their regulation by estrogen in Atlantic croaker

Cited by 24 publications

References 48 publications

Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis

Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis

Molecular cloning, characterization and expression of FSH and LH beta subunits from grass carp (Ctenopharyngodon idella)

Occurrence of follicle-stimulating hormone-like substance in the Kuruma prawn,Marsupenaeus japonicus, during ovarian maturation

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