Molecular cloning of glutathione peroxidase gene of Antarctic ice microalga Chlamydomonas sp. ICE‐L and its expression changes under temperature and salinity stress
Abstract:A complete open reading frame (ORF) encoding a glutathione peroxidase (ICE-LGPx) was determined from Antarctic Chlamydomonas sp. ICE-L by the molecular biological methods. The transcriptional expression patterns of ICE-LGPx under different salinity and temperature stresses were determined by the real-time polymerase chain reaction, and the activities of ICE-LGPx were assayed under salinity stress as well. The fulllength cDNA sequence of ICE-LGPx mRNA was 1956 bp including an ORF of 765 bp. Molecular structure … Show more
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