Molecular Cloning of Humand-Dopachrome Tautomerase cDNA: N-terminal Proline Is Essential for Enzyme Activation

Nishihira, Jun; Fujinaga, Mami; Kuriyama, Taisuke; Suzuki, Masaki; Sugimoto, Hiroshi; Nakagawa, Atsushi; Tanaka, Isao; Sakai, Masaharu

doi:10.1006/bbrc.1998.8123

Cited by 41 publications

(29 citation statements)

References 23 publications

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“…2A). D-dopachrome tautomerase has a molecular weight of 12 kDa, and is highly expressed in the liver, but has also been found in the kidneys, brain, spleen, lung and heart (Odh et al 1993;Nishihira et al 1998). The protein shares homology (33%) with the macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine involved in many inflammatory and immune responses (Zhang et al 1995;Orita et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat

et al. 2008

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Proteomic techniques such as two-dimensional gel electrophoresis (2-DGE) and mass spectrometry have become important tools for the identification of novel biomarkers of toxicity and disease. Ideally, such biomarkers need to be sensitive and organ specific, but, recently, it has become apparent that it would be an additional benefit to be able to measure biomarkers in samples obtained using non-invasive methods. The present study is concerned with the identification of novel urinary markers of hepatic fibrosis. In a carbon-tetrachloride-induced liver fibrosis rat model, analysis of urine by 2-DGE revealed an increase in the concentration of a number of proteins in animals with hepatic fibrosis. Using in-gel trypsin digest and nano-scale liquid chromatography combined with electrospray ionisation tandem mass spectrometry, protein spots were identified as copper/zinc superoxide dismutase, D: -dopachrome tautomerase, beta-2-microglobulin and neutrophil gelatinase associated lipocalin. These proteins are known to have important roles in the inflammatory response.

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Section: Discussionmentioning

confidence: 99%

Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat

et al. 2008

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“…The conversion of -dopachrome into 5,6-dihydroxyindole is the only known enzymic activity of the enzyme DDCT [22,23]. Since -dopachrome is not naturally occurring, it is highly likely that the enzyme has another, yet to be discovered, function and may in the future be more appropriately renamed.…”

Section: Discussionmentioning

confidence: 99%

“…DDCT converts 2-carboxy-2,3-dihydroindole-5,6-quinone (-dopachrome) into 5,6-dihydroxyindole, and is expressed widely in human tissues [22,23]. Additional sequence from the 5h ends of the GSTT2 and GSTT2P genes was obtained from T2cos8 and T2cos2, and it was noted that both 5h flanking sequences contained the start of a gene encoding DDCT.…”

Section: Ddctmentioning

confidence: 99%

Structure and organization of the human Theta-class glutathione S-transferase and d-dopachrome tautomerase gene complex

Coggan

Whitbread

Whittington

et al. 1998

Biochemical Journal

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The structure and organization of the human Theta-class glutathione S-transferase (GST) genes have been determined. GSTT1 and GSTT2 are separated by approx. 50 kb. They have a similar structure, being composed of five exons with identical exon/intron boundaries. GSTT1 is 8.1 kb in length, while GSTT2 is only 3.7 kb. The GSTT2 gene lies head-to-head with a gene encoding d-dopachrome tautomerase (DDCT), which extends over 8.5 kb and contains four exons. The sequence between GSTT2 and DDCT may contain a bidirectional promoter. The GSTT2 and DDCT genes have been duplicated in an inverted repeat. Sequence analysis of the duplicated GSTT2 gene has identified an exon 2/intron 2 splice site abnormality and a premature translation stop signal at codon 196. These changes suggest that the duplicate gene is a pseudogene, and it has been named GSTT2P.

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“…To examine the biological link between isomerase activity and biological functions, cDNA of the P1A mutant of MIF was prepared using a site-directed mutagenesis technique as described previously (26). In brief, the sense primer designed was 5Ј-CATATGGGCATGTTCATCGTA-3Ј (1-18) containing the 5Ј-end sequence encoding an alanine instead of a proline after the initiating methionine with an NdeI restriction site, and the antisense primer was 5Ј-GGATCCTTAGGCGAAGGTGGAGTT-3Ј (331-348) containing a 3Ј-end sequence identical to the wild-type cDNA with a BamHI restriction site.…”

Section: Methodsmentioning

confidence: 99%

Macrophage Migration Inhibitory Factor Up-regulates Expression of Matrix Metalloproteinases in Synovial Fibroblasts of Rheumatoid Arthritis

Onodera¹,

Kaneda²,

Mizue³

et al. 2000

Journal of Biological Chemistry

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Molecular Cloning of Humand-Dopachrome Tautomerase cDNA: N-terminal Proline Is Essential for Enzyme Activation

Cited by 41 publications

References 23 publications

Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat

Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat

Structure and organization of the human Theta-class glutathione S-transferase and d-dopachrome tautomerase gene complex

Macrophage Migration Inhibitory Factor Up-regulates Expression of Matrix Metalloproteinases in Synovial Fibroblasts of Rheumatoid Arthritis

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