Molecular cloning of Kuruma shrimp Marsupenaeus japonicus endonuclease-reverse transcriptase and its positive role in white spot syndrome virus and Vibrio alginolyticus infection

Ma, Xiongchao; Sun, Baozhen; Zhu, Faming

doi:10.1016/j.fsi.2017.12.031

Cited by 17 publications

(5 citation statements)

References 29 publications

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“…in shrimp intestines is higher than its metabolic activity. This is in agreement with previous studies, where innate immune system activation of shrimp, such as Marsopenaeus japonicus and L. vannamei, was evaluated in response to V. alginolyticus and V. parahaemolyticus infections, demonstrating that they could modulate host signalling proteins and activate their defence system in the presence of potentially pathogenic microorganisms, activating genes that have activities related to immune response mechanisms, such as prophenoloxidase and superoxide dismutase activities, and apoptosis in the hepatopancreas tissue 36,37 as well as in the intestinal epithelium 38 . www.nature.com/scientificreports/ Therefore, a selective biological pressure exerted by the host on the metabolic activity of this bacteria could be occurring, while stimulating the shrimp's immune system by functioning as a natural probiotic necessary for the life cycle of the host.…”

Section: Discussionsupporting

confidence: 92%

The implication of metabolically active Vibrio spp. in the digestive tract of Litopenaeus vannamei for its post-larval development

Garibay-Valdez

Martínez‐Córdova

López-Torres

et al. 2020

Sci Rep

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This work aimed to evaluate the link between the occurrence/abundance of Vibrio populations and bacterial composition in shrimp's intestine (Litopenaeus vannamei) during post-larval ontogenetic development and in its culture water, and the correlation of these with environmental parameters. The total and metabolically active populations of Vibrio in the digestive tract of shrimp during its post-larval development were analysed using quantitative PCR (qPCR) and reverse transcription qPCR targeting the 16S rRNA gene sequence. A lab-scale shrimp bioassay was performed for 80 days in a recirculating aquarium under strictly controlled conditions. The results indicate that the Vibrio population from shrimp's gut is associated with its developmental stage and the environment. Multivariate analyses revealed that the presence of Vibrio spp. drove the studied system, but their metabolically active performance was related to earlier developmental stages in an aqueous environment. Also, the samples taken from water of culture units to compare the influence of the aquatic environment on the intestinal microbial community during shrimp's ontogenetic development showed significant differences. Finally, our results revealed that Vibrio is an important member of shrimp's gut microbiota; however, its metabolic activity seems to be highly regulated, possibly by the host and by the rest of the microbiota. Shrimp aquaculture plays an important role in the world's economy. In the western hemisphere, Mexico is the sixth-largest producer of shrimp by aquaculture and, specifically, 37% of this production is provided by the State of Sonora, where the Pacific white shrimp, Litopenaeus vannamei is one of the most exported species around the world for human consumption due to its edible protein and nutrients 1,2. In the last decade, the high demand from the aquaculture market, the quickly growing rate of shrimp production, and intensive farming practices have made these shrimp susceptible to disease and mortality, causing spoiled cultures and irreparable economic losses for the aquaculture industry 2-4. One of the principal factors threatening shrimp production is related to biotic factors, which encompass virus and bacterial infections, and abiotic factors which are mostly linked to intensive farming practices, triggering the accumulation of carbonaceous, nitrogenous, and phosphorous species in farming ponds 1,3. Shrimp diseases caused by opportunistic bacteria such as bacteria and viruses are the major problems that can lead to considerable losses in the shrimp farm industry. The main shrimp diseases are caused by bacterial pathogens mainly from Vibrio species such as V. harveyi, V. alginolyticus, V. campbellii, and V. parahaemolyticus, causing necrosis, slow growth, anorexia, and mortality during the post-larval development 5. In this regard V. parahaemolyticus strains constitute the etiological agent of the most relevant bacterial disease recorded in shrimp to this day, which has severely disrupted the shrimp industry containing a unique...

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Section: Discussionsupporting

confidence: 92%

The implication of metabolically active Vibrio spp. in the digestive tract of Litopenaeus vannamei for its post-larval development

Garibay-Valdez

Martínez‐Córdova

López-Torres

et al. 2020

Sci Rep

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“…Many genes either inhibit viral replication or facilitate viral pathogenesis [33]. Endonuclease-reverse transcriptase in M. japonicus promotes anti-WSSV immunity by regulating superoxide dismutase activity, apoptosis, and phenoloxidase activity [34]. The Lvc-Jun gene could upregulate the activity of the wsv249 promoter to facilitate viral replication [35].…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of a novel L-type lectin (MjLTL2) from kuruma shrimp (Marsupenaeus japonicus)

Jing

Liu

et al. 2020

Fish & Shellfish Immunology

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L-type lectins (LTLs) belong to the lectin family and are characterized by a conserved structural motif in their carbohydrate recognition domain. LTLs are homologous to leguminous lectins. In this study, we identified and functionally characterized an LTL from kuruma shrimp Marsupenaeus japonicus. We designated this LTL as MjLTL2. MjLTL2 contains a signal peptide, a Lectin_leg domain, a coiled coil, and transmembrane domain. MjLTL2 is distributed in hemocytes, heart, hepatopancreas, gill, stomach, and intestine; higher expression levels are seen in hemocytes and the hepatopancreas than in other tissues. MjLTL2 was upregulated following challenge of shrimp with Vibrio anguillarum and white spot syndrome virus (WSSV). MjLTL2 can agglutinate several bacteria without Ca 2+ . In addition, MjLTL2 could bind to several Gram-positive and -negative bacteria by binding to their lipopolysaccharide and peptidoglycan. However, MjLTL2 could not enhance the clearance of V. anguillarum in vivo. In the presence of WSSV infection, MjLTL2 knockdown by RNA interference resulted in a 7day lower cumulative mortality of M. japonicus. Moreover, less VP19, VP24, VP26, and VP28 mRNAs were extracted from the hemocytes of MjLTL2 knockdown shrimp than from the control. These results suggest that MjLTL2 is involved in immune responses in shrimp.

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“…However, Zheng et al (2017) demonstrated that all the dominating vibrio in the diseased shrimp were not pathogenic. Recently, Garibay-Valdez et al (2018) reported that Vibrio spp maintain symbiotic relationship with host and gut microbiota in addition to modulating host signaling proteins, activate defense system and improve immune response like prophenoloxidase and superoxide dismutase activities (Ma et al 2018;Qi et al 2017;Velázquez et al 2019). Further, Vibrios like V. gazogenes have been reported to confer resistance to bacterial infection suggesting their potential probiotic activity (Thompson et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Gut microbial diversity and composition of Indian white shrimp (Penaeus indicus)

Patil

Ghate

et al. 2021

Preprint

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The endemic Indian white shrimp (Penaeus indicus) is an economically important shrimp species, distributed in the Indo-West Pacific region. Knowledge of gut microbial composition helps in dietary interventions to ensure improved health and production. Here we analyzed V3-V4 hypervariable regions of the 16S rRNA gene to examine intestinal microbiota in the wild and farmed P. indicus. The study revealed Proteobacteria, Fusobacteria, Tenericutes, and Bacteroidetes, were the dominant phyla in both the groups; though the differences in relative abundance were evident. The dominant genera in case of the wild group were Photobacterium (29.5%) followed by Propionigenium (13.9%), Hypnocyclicus (13.7%) and Vibrio (11.1%); while Vibrio (46.5%), Catenococcus (14%), Propionigenium (10.3%) and Photobacterium (8.7%) were dominant in the farmed group. The results of the study suggest the role of environment on the relative abundance of gut bacteria. This is the first report characterizing gut microbial diversity in P. indicus, which can be used to understand the role of gut microbiota in health, nutrition, reproduction, and growth.

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Molecular cloning of Kuruma shrimp Marsupenaeus japonicus endonuclease-reverse transcriptase and its positive role in white spot syndrome virus and Vibrio alginolyticus infection

Cited by 17 publications

References 29 publications

The implication of metabolically active Vibrio spp. in the digestive tract of Litopenaeus vannamei for its post-larval development

The implication of metabolically active Vibrio spp. in the digestive tract of Litopenaeus vannamei for its post-larval development

Identification and characterization of a novel L-type lectin (MjLTL2) from kuruma shrimp (Marsupenaeus japonicus)

Gut microbial diversity and composition of Indian white shrimp (Penaeus indicus)

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