Molecular Cloning of Newt Prolactin (PRL) cDNA: Effect of Temperature on PRL mRNA Expression

Takahashi, Noriyuki; Hasunuma, Itaru; Iwata, Takeo; Umezawa, Kuniaki; Yamamoto, Kazutoshi; Marin, Antoine; Perroteau, Isabelle; Vellano, Camillo; Kikuyama, Sakaé

doi:10.1006/gcen.2000.7594

Cited by 13 publications

(7 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Assuming that 8 weeks of acclimation results in a steady resetting of the protein expression machinery in a cell, we speculate that differences in mRNA levels reflect equivalent differences in protein production and secretion, implying that 29°C-acclimated carp have lower plasma prolactin levels (which we cannot determine directly by radioimmunoassay) than the 15 and 22°C-acclimated fish. This is in agreement with plasma measurements in rainbow trout Oncorhynchus mykiss (Rand-Weaver et al, 1995) and newt Cynops pyrrhogaster (Takahashi et al, 2001) Regulation of branchial Na + /K + -ATPase in carp permeability to water and ions (Hirano, 1986;Wendelaar Bonga et al, 1990;Evans, 2002), our observation that 29°C-acclimated fish have a higher apparent Na + /K + -ATPase activity is in accordance with an enhanced branchial permeability as a result of downregulated prolactin expression, leaving plasma mineral composition within a physiological range.…”

Section: Cortisol and Prolactinsupporting

confidence: 91%

Regulation of branchial Na+/K+-ATPase in common carpCyprinus carpioL. acclimated to different temperatures

Metz

Burg

Bonga

et al. 2003

Journal of Experimental Biology

113

View full text Add to dashboard Cite

Isogenic carp Cyprinus carpio L. were acclimated to water temperatures of 15, 22 and 29°C for at least 8 weeks. The acclimations consistently resulted in slightly, but significantly, different plasma osmolality, sodium, potassium and chloride concentrations between the groups studied. Plasma total and ionic calcium levels were unaffected, indicating successful adaptation. The apparent changes in set point for plasma ion levels are explained by altered sodium pump activity and hormonal control of branchial permeability to water and ions. It appears that in 15°C-acclimated fish, a lower apparent Na + /K + -ATPase activity is compensated by strongly enhanced Na + /K + -ATPase expression (determined biochemically and immunohistochemically). In 29°C-acclimated fish, the higher ambient temperature activates the enzyme. Arrhenius plots for branchial Na + /K + -ATPase preparations of the three groups of fish suggest the occurrence of different enzyme isoforms or protein (in)stability as explanations for differences in apparent enzyme activities, rather than temperature-dependent changes in membrane fluidity. As for hormonal control over permeability, prolactin mRNA expression (and anticipated production and release) is lower in fish kept at 29°C, suggesting that control over branchial permeability to water and ions needs to be downregulated at higher temperatures. In so doing, enhanced sodium pump activity is balanced by a controlled passive ion loss to fine-tune plasma sodium levels. Basal plasma cortisol levels did not correlate positively with Na + /K + -ATPase expression, but doubling plasma cortisol levels in control fish by administering exogenous cortisol (for 7 days, using implanted minipumps and thus stress-free) enhanced Na + /K + -ATPase expression. This effect must be the result of a glucocorticoid action of the steroid: in fish, mineralocorticoid receptors have higher affinity for cortisol than glucocorticoid receptors. At a lower ambient temperature, branchial Na + /K + -ATPase expression is upregulated to counteract the temperature-inhibited activity of the sodium pump, perhaps via a mineralocorticoid receptor.

show abstract

Section: Cortisol and Prolactinsupporting

confidence: 91%

Regulation of branchial Na+/K+-ATPase in common carpCyprinus carpioL. acclimated to different temperatures

Metz

Burg

Bonga

et al. 2003

Journal of Experimental Biology

113

View full text Add to dashboard Cite

show abstract

“…Sex pheromone production is known to be affected by temperature in red-bellied newts, Cynops pyrrhogaster, where low temperatures (8-12°C) induce increased synthesis of prolactin mRNA, and prolactin, in conjunction with androgens, can induce hypertrophy of the secretory capacity of the newt pheromone (sodefrin) gland and stimulate production of sodefrin (Toyoda et al 1994;Yamamoto et al 1996;Iwata et al 2000a;Takahashi et al 2001). Sodefrin is a peptide pheromone, thus the mechanisms controlling its synthesis and expression will differ from those acting in garter snakes to produce the relatively small methyl ketone molecules that act as pheromones in our system.…”

Section: Discussionmentioning

confidence: 99%

“…In all vertebrates studied to date with described or putative pheromones, pheromone production and expression are thought to be controlled almost exclusively by hormones (e.g., amphibians, Yamamoto et al 1996;Iwata et al 2000a;Kikuyama et al 2005;reptiles, Mendonça and Crews 1996;birds, Rajchard 2007;rodents, Bruce 1965;Thiessen et al 1968;Mugford and Nowell 1971;primates, Michael 1975;goats, Iwata et al 2000b). However, in at least one vertebrate, the red-bellied newt, Cynops pyrrhogaster, pheromone production may be promoted by low temperature (Iwata et al 2000a;Takahashi et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Low Temperature Dormancy Affects the Quantity and Quality of the Female Sexual Attractiveness Pheromone in Red-sided Garter Snakes

Parker

Mason

2009

J Chem Ecol

View full text Add to dashboard Cite

Low temperature dormancy is a necessary requirement of the annual cycle of most nonmigratory, temperate vertebrates. The red-sided garter snake, Thamnophis sirtalis parietalis, overwinters in communal dens during its prolonged winter dormancy (8 mo), and upon emergence, reproductive behavior of both sexes is maximal. Previous work on this species showed that male courtship behavior is maximally induced after simulated low temperature dormancy. The purpose of this study was to determine whether low temperature dormancy affects the pheromone profiles of individual female red-sided garter snakes. We collected females in the fall at den sites in Manitoba, Canada, and extracted pheromones from individuals at three different time points: fall (field), winter (lab), and spring (lab). Total skin lipid and pheromone fraction masses increased from fall to spring, and pheromone profiles were distinctly different in the fall and spring. Pheromone profiles became dominated by the long-chain, unsaturated methyl ketone components of the blend by the time snakes emerged in the spring. Further, the amounts of both saturated and unsaturated components increased from fall to spring, suggesting significant sex pheromone synthesis was induced by low temperature dormancy.

show abstract

“…In general, PRL-secreting cells are the earliest of the adenohypophysial cells (thyrotrope, melanotrope, adrenocorticotrope, somatotrope, somatolactotrope, gonadotrope) to appear in freshwater teleosts (as in the salmoniforms and the cyprinidontiforms: Ruijter & Weenderlaar Bonga 1987, Mal et al 1989, Naito et al 1993, Saga et al 1993 and brackish water teleosts (as in some perciforms -gilthead sea bream and Oreochromis mossambicus : Power & Canario 1992, Ayson et al 1994, Villaplana et al 2000. Also, in amphibians PRL is possibly one of the most important adenohypophysial hormones in early development, being responsible for many functional processes, for example osmoregulation of the embryo or very young larvae (Takahashi et al 2001). Hence, we show in this work a tendency towards the late appearance of PRL cells as was demonstrated in amniotic animals and seawater teleosts (e.g.…”

Section: Discussionmentioning

confidence: 99%

Transient expression pattern of prolactin in Sparus aurata

Herrero-Turrión

Rodríguez

Aijón³

et al. 2003

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Using reverse transcription-polymerase chain reaction and in situ hybridization, the expression of the prolactin (PRL) gene was determined during development in gilthead sea bream (Sparus aurata) for the first time. The mRNA for PRL was detected from the second day of the larval stage onwards. This transcript was also located in the adenohypophysial cells, starting at four days post-hatching and was found to be pituitary-specific. Moreover, the possible involvement of PRL in asynchronous growth in the cultivation of gilthead sea bream was also examined. No differences in the distribution of PRL cells were observed in the three sizes of juvenile gilthead sea bream studied. These results suggest that the transcription of PRL is involved in the early development stages of sea bream and that the asynchronous growth-related changes are not due to distinct distribution of PRL cells.

show abstract

Molecular Cloning of Newt Prolactin (PRL) cDNA: Effect of Temperature on PRL mRNA Expression

Cited by 13 publications

References 46 publications

Regulation of branchial Na+/K+-ATPase in common carpCyprinus carpioL. acclimated to different temperatures

Regulation of branchial Na+/K+-ATPase in common carpCyprinus carpioL. acclimated to different temperatures

Low Temperature Dormancy Affects the Quantity and Quality of the Female Sexual Attractiveness Pheromone in Red-sided Garter Snakes

Transient expression pattern of prolactin in Sparus aurata

Contact Info

Product

Resources

About