Molecular cloning of phenylalanine ammonia-lyase cDNA fromPisum sativum

Kawamata, Shinji; Yamada, Tetsuji; Tanaka, Yoshikazu; Sriprasertsak, Permpong; Kato, Hisayoshi; Ichinose, Yuki; Kato, Hidenori; Shiraishi, Takeshi; Oku, Hachiro

doi:10.1007/bf00029163

Cited by 29 publications

(14 citation statements)

References 18 publications

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“…In addition to the sad cDNA clones (see below), the following cDNA clones were used to probe the northern blots for the occurrence of their specific mRNAs: the cab cDNA clone (pAB96), which corresponds to the nuclear gene encoding the chloroplast-localized chlorophyll a/bbinding protein (Coruzzi et al, 1983); the probe for chalcone synthase (the pCC6 cDNA pea clone; Ichinose et al, 1992); the PAL cDNA clone (cPAL-1; Kawamata et al, 1992). An 18S rRNA probe (construct termed pÅS3; Kalbin et al, 1997) was used to check even loading of total RNA on the agarose gel and the transfer efficiency.…”

Section: Northern Blottingmentioning

confidence: 99%

Cloning, Expression, and Molecular Characterization of a Small Pea Gene Family Regulated by Low Levels of Ultraviolet B Radiation and Other Stresses

Brosché

Strid

1999

Plant Physiology

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A pea (Pisum sativum) DNA fragment (termed MB3) was isolated by differential display of cDNAs obtained from total leaf RNA of ultraviolet B (UV-B) radiation-treated plants. Longer cDNAs were cloned by rapid amplification of cDNA ends in the 3 to 5 direction. Three different, but very similar, cDNAs were cloned, sadA, sadB, and sadC, the major difference between them being a 36-bp deletion in the coding region of sadB. Southern blotting confirmed the occurrence of at least three genes in the pea genome. Database comparisons of the SAD protein sequences revealed high identity (46%) and similarity (77%) with a putative tomato (Lycopersicon esculentum) short-chain alcohol dehydrogenase. Very low levels of UV-B radiation (the biologically effective radiation normalized to 300 nm ‫؍‬ 0.08 W m ؊2 ) was shown to up-regulate expression, a dose considerably lower than that needed to induce expression of the well-known UV-B defensive chalcone synthase and phenylalanine ammonia lyase genes. RNase protection assay revealed that primarily sadA and sadC mRNA accumulation was enhanced by UV-B. In addition to UV-B irradiation, ozone fumigation, wounding, aluminum stress, and salt stress induced increased transcript levels of the sad genes in pea.Exposure of plants to UV-B radiation (280-320 nm) results in deleterious effects in plant cells and to the switch-on of defense programs in order to minimize the effects. In particular, the stress responses are directed to protect the genetic content of the nucleus. The adverse effects and the defense responses have been partly characterized in several plant species. For instance, a number of Arabidopsis mutants, hypersensitive to UV-B radiation, have been isolated. These mutants show defects in several types of cellular functions, such as flavonoid biosynthesis (Li et al., 1993;Rao and Ormrod, 1995) and DNA repair (Britt et al., 1993). These mutants indicate what metabolic routes are important for UV-B tolerance in plants.In pea (Pisum sativum) plants, the effects discovered can roughly be divided into two groups: (a) deleterious effects on chloroplast components, chiefly those involved in photosynthesis (Chow et al

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Section: Northern Blottingmentioning

confidence: 99%

Cloning, Expression, and Molecular Characterization of a Small Pea Gene Family Regulated by Low Levels of Ultraviolet B Radiation and Other Stresses

Brosché

Strid

1999

Plant Physiology

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“…The deduced tobacco PAL.E protein shares, at the amino acid level, a degree of similarity higher than 80% with those deduced from cDNA clones isolated from dicotyledonous species such as bean (Edwards et al, 1985), parsley (Lois et al, 1989), alfalfa (Gowri et al, 1991), potato (Joos and Hahlbrock, 1992), pea (Kawamata et al, 1992), loblolly pine (Wetten and Sederoff, 1992), and poplar (Subramaniam et al, 1993). When this paper was in process a very similar cDNA clone was isolated from tobacco cell culture (Nagai et al, 1994).…”

Section: Isolation and Sequence Analysis Of Pal Cdna Clonesmentioning

confidence: 99%

Phenylalanine Ammonia-Lyase in Tobacco (Molecular Cloning and Gene Expression during the Hypersensitive Reaction to Tobacco Mosaic Virus and the Response to a Fungal Elicitor)

et al. 1994

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“…Ten mg of total RNA was denatured, fractionated by electrophoresis on a 1% agarose gel and then blotted onto a nylon membrane filter with a downward alkaline capillary transfer method (Chomczynski 1992). Using S64 and PsPAL1 cDNA clones (D10001, Kawamata et al 1992), DIG-labeled S64 and PAL RNA probes were made with an RNA Transcription Kit (Stratagene) using the DIG RNA Labeling Mix (Boehringer Mannheim, Mannheim, Germany). Hybridization was performed at 68°C overnight in a solution containing 50% (v/v) formamide, 5´ SSC, 0.1% laurylsarcosine, 0.02% SDS (w/v) and 2% blocking solution (Boehringer Mannheim).…”

Section: Northern Blotting Analysismentioning

confidence: 99%

Expression of the 12-Oxophytodienoic Acid 10,11-Reductase Gene in the Compatible Interaction between Pea and Fungal Pathogen

Ishiga

Funato

Tachiki

et al. 2002

Plant and Cell Physiology

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;Suppressors produced by Mycosphaerella pinodes are glycopeptides to block pea defense responses induced by elicitors. A clone, S64, was isolated as cDNA for suppressorinducible gene from pea epicotyls. The treatment of pea epicotyls with suppressor alone induced an increase of S64 mRNA within 1 h, and it reached a maximum level at 3 h after treatment. The induction was not affected by application of the elicitor, indicating that the suppressor has a dominant action to regulate S64 gene expression. S64 was also induced by inoculation with a virulent pathogen, M. pinodes, but not by inoculation with a non-pathogen, Ascochyta rabiei, nor by treatment with fungal elicitor. The deduced structure of S64 showed high homology to 12-oxophytodienoic acid reductase (OPR) in Arabidopsis thaliana. A recombinant protein derived from S64 had OPR activity, suggesting compatibility-specific activation of the octadecanoid pathway in plants. Treatment with jasmonic acid (JA) or methyl jasmonic acid, end products of the octadecanoid pathway, inhibited the elicitor-induced accumulation of PAL mRNA in pea. These results indicate that the suppressor-induced S64 gene expression leads to the production of JA or related compounds, which might contribute to the establishment of compatibility by inhibiting the phenylpropanoid biosynthetic pathway.

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Molecular cloning of phenylalanine ammonia-lyase cDNA fromPisum sativum

Cited by 29 publications

References 18 publications

Cloning, Expression, and Molecular Characterization of a Small Pea Gene Family Regulated by Low Levels of Ultraviolet B Radiation and Other Stresses

Cloning, Expression, and Molecular Characterization of a Small Pea Gene Family Regulated by Low Levels of Ultraviolet B Radiation and Other Stresses

Phenylalanine Ammonia-Lyase in Tobacco (Molecular Cloning and Gene Expression during the Hypersensitive Reaction to Tobacco Mosaic Virus and the Response to a Fungal Elicitor)

Expression of the 12-Oxophytodienoic Acid 10,11-Reductase Gene in the Compatible Interaction between Pea and Fungal Pathogen

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