Molecular cloning of sea bass (Dicentrarchus labrax L.) caspase-8 gene and its involvement in Photobacterium damselae ssp. piscicida triggered apoptosis

The bacterial exotoxin AIP56 induces fish macrophage and neutrophil apoptosis using mechanisms of the extrinsic and intrinsic pathways

“…The extrinsic and intrinsic pathways are the major and best characterized pathways involved in the progress of apoptosis [59] and although distinct they can be concurrent. The present results showing that caspase-8 and -9 are activated, and cytochrome c translocated from mitochondria to the cytosol, together with the observation that expression of caspase-8 occurs early in the process [54], suggest that rAIP56-induced apoptosis involves both the extrinsic and intrinsic apoptotic pathways.…”

“…Therefore these caspase-8 and -9 inhibition results cannot be used to establish the initiator caspase in the apoptotic process here described. Nonetheless, when a kinetic study measuring the expression of caspase-8, -9 and -3 by real-time PCR was performed in one of the Phdp targeted organs, the spleen, following inoculation of sea bass with AIP56-containing mid-exponential phase Phdp culture supernatants, the results showed that the expression levels of caspase-8 and -3 increased earlier than those of caspase-9 [54].…”

Molluscan death effector domain (DED)-containing caspase-8 gene from disk abalone (Haliotis discus discus): Molecular characterization and expression analysis

“…In sea bass fish, caspase-8 is constitutively expressed in all tested tissues [27]. Also, invertebrate shrimp and the variously-colored abalone (H. diversicolor) have shown caspase expression in all tissues tested.…”

“…Sea bass caspase-8 expression was increased in spleen by Photobacterium damelae spp. piscicida infection [27]. In marine shrimp Marsupenaeus japonicas, caspase was significantly up-regulated in hepatopancreas during infection with white spot syndrome virus (WSSV) [30].…”

Effect of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infection on caspase 3c expression and activity in freshwater prawn Macrobrachium rosenbergii

“…Among all the caspases, caspase 8 serves as the key regulator of the entire apoptosis process as it is initiated upon activation by Fas ligand, tumour necrosis factor receptor 1 (TNFR1), and related death receptors of the tumour necrosis factor (TNF) superfamily [19]. Caspase 2 exhibits both the characteristics of initiator and effector of caspases and promotes apoptosis in some cells [8].…”