Molecular cloning of the F8 fimbrial antigen from Escherichia coli

Hacker, J

doi:10.1016/0378-1097(86)90300-9

Cited by 8 publications

(17 citation statements)

References 12 publications

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“…Among P fimbriae, the serotypes F7 through F13 have been distinguished (27). From uropathogenic E. coli, the genes responsible for synthesis of various serologically different P fimbriae, i.e., F71, F72, F8, F9, Fll, and F13, have been cloned (5,6,10,12,28,36,37). It has been shown that these fimbrial gene clusters are very similar in general organization (25,36,38), which suggests that the biogenesis of P fimbriae may follow a general concept.…”

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confidence: 99%

Genetic manipulation of major P-fimbrial subunits and consequences for formation of fimbriae

Die¹,

Wauben²,

Megen³

et al. 1988

J Bacteriol

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was studied. Deletion of two regions that code for hypervariable parts of the P fimbrillin resulted in strong reduction or total absence of fimbria production. Replacement of deleted amino acids by other amino acid residues restored the formation of fimbriae. The hypervariable regions may be important for biogenesis of fimbriae by imposing correct spacing between conserved regions of the protein.The potential for substituting amino acids in the P-fimbrial subunit opens interesting possibilities for use of fimbriae as carriers of foreign antigenic determinants. An antigenic determinant of foot-and-mouth disease virus (FMDV) was incorporated in the F11 fimbrial subunit. Hybrid fimbriae, recognized by an FMDV-specific neutralizing monoclonal antibody directed against FMDV, were formed.Fimbriae are long filamentous appendages that occur on many bacteria and consist of about 1,000 protein subunits (for a review, see reference 14). Many uropathogenic Escherichia coli strains expose on their cell surfaces P fimbriae that mediate adherence of the pathogen to the uroepithelium (11,31,32). P fimbriae recognize the a-D-galactose-(1,4)-P-D-galactose moiety of antigens of the P blood group (13,18). Among P fimbriae, the serotypes F7 through F13 have been distinguished (27). From uropathogenic E. coli, the genes responsible for synthesis of various serologically different P fimbriae, i.e., F71, F72, F8, F9, Fll, and F13, have been cloned (5,6,10,12,28,36,37). It has been shown that these fimbrial gene clusters are very similar in general organization (25,36,38), which suggests that the biogenesis of P fimbriae may follow a general concept.The composition of P fimbriae is very complex. The major subunit, or P fimbrillin, is predominant and determines the antigenic properties (35). Several minor components, among them the adhesin protein, are also present in the fimbrial structure (19,20,29 MATERIALS AND METHODSBacterial strains, plasmids, and growth conditions. E. coli K-12 HB101 (2), a strain deficient in the production of type 1 fimbriae, was used as the host strain in all experiments unless otherwise indicated. JM101 was used as the host strain for M13mp8 derivatives (24). HB2154 (3) was used as the host strain for M13mpl8 (26) derivatives in the localized mutagenesis experiments. The mutant plasmids described were derived from pPIL110-75, carrying thefso gene cluster, and pPIL291-15, carrying the fel (F-eleven) gene cluster (6,29,36

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confidence: 99%

Genetic manipulation of major P-fimbrial subunits and consequences for formation of fimbriae

Die¹,

Wauben²,

Megen³

et al. 1988

J Bacteriol

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“…A cosmid gene library of strain 2980 was constructed as described [11]. Mannose-resistant and mannose-sensitive hemagglutinating clones were selected and characterized (see Table 1).…”

Section: Isolation Of Cosmid Clonesmentioning

confidence: 99%

“…The DNAs were treated with suitable restriction enzymes and the resulting fragments were separated by agarose gel electrophoresis using 0.7-1% gels [11]. The transfer of DNA fragments from agarose gels to nitrocellulose filters, washing and autoradiography were performed as described by Southern [21].…”

Section: Nick Translation Southern Hybridization and Autoradiographymentioning

confidence: 99%

“…DNA fragments were 317 labeled by nick-translation with a m~xture of all 4 c~-32p-labeled dNTPs and purified by ethanol precipitation. As DNA probes a 6.0 Kb Kpnl-HindlII fragment derived from plasmid pANN921 (specific for the P determinants; see [11]) and a 4.2 Kb…”

Section: Nick Translation Southern Hybridization and Autoradiographymentioning

confidence: 99%

“…In contrast, type I fimbriae (F 1A) which recognizes a mannose-containing receptor are termed mannose-sensitive hemagglutination (MSH) fimbriae [5]. Genetic determinants which code for some of the P-specific fimbriae as well as for type I fimbriae have been cloned and analysed [6][7][8][9][10][11][12]. It has been described that in some uropathogenic E. coli strains copies of P-specific determinants were found to exist at multiple sites of the chromosome [13,14] whereas type I specific genes seem to be located at a fixed position on the E. coli chromosome [13,15, unpublished results].…”

Section: Introductionmentioning

confidence: 99%

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Chromosomal mapping of genes encoding mannose-sensitive (type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli O18:K5:H5

Krallmann-Wenzel¹

1989

FEMS Microbiology Letters

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Antiadhesive hydroalcoholic extract from Apium graveolens fruits prevents bladder and kidney infection against uropathogenic E. coli

et al. 2018

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Fruits from Apium graveolens (Celery) are used traditionally in Persian and European medicine for the treatment of uncomplicated urinary tract infections. No data are available on A. graveolens extract on the interplay between uropathogenic E. coli and the eukaryotic host cells and on quorum sensing of the bacteria. The present study aimed to characterize an antiadhesive and anti quorum sensing effect of a characterized A. graveolens extract by specific in vitro assays and to correlate these effects with in vivo data obtained by an animal infection model. Hydroalcoholic extract CSE (EtOH-water, 1:1) from A. graveolens fruits was characterized by UHPLC/+ESI-QTOF-MS and investigated on antiproliferative activity against UPEC (strain NU14) and human T24 bladder cells. Antiadhesive properties of CSE were investigated within two different in vitro adhesion assays. For in vivo studies BALB/c mice were used in an UPEC infection model. The effect of CSE on bacterial load in bladder tissue was monitored within a 4- and 7 days pretreatment (200, 500 mg/kg) of the animals. CSE was dominated by the presence of luteolin-glycosides and related flavons besides furocoumarins. CSE had no cytotoxic effects against UPEC and bladder cells. CSE exerts a dose dependent antiadhesive activity against UPEC strains NU14 and UTI89. CSE inhibited in a concentration-dependent manner bacterial quorum sensing. 4- and 7-day pretreatment of animals with CSE transurethrally infected with UPEC NU14, significantly reduced the bacterial load in bladder tissue. CSE is assessed as an antiadhesive extract for which the traditional use in phytotherapy for UTI is justified.

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Molecular cloning of the F8 fimbrial antigen from Escherichia coli

Cited by 8 publications

References 12 publications

Genetic manipulation of major P-fimbrial subunits and consequences for formation of fimbriae

Genetic manipulation of major P-fimbrial subunits and consequences for formation of fimbriae

Chromosomal mapping of genes encoding mannose-sensitive (type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli O18:K5:H5

Antiadhesive hydroalcoholic extract from Apium graveolens fruits prevents bladder and kidney infection against uropathogenic E. coli

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