Molecular cloning, structural analysis, and expression of carp ZP2 gene

Chang, Ya-Wen; Hsu, Chen-Yi; Wang, S.C.; Tsao, Chia Wen; Huang, Fore‐Lien

doi:10.1002/(sici)1098-2795(199703)46:3<258::aid-mrd4>3.0.co;2-o

Cited by 92 publications

(65 citation statements)

References 34 publications

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“…In particular, the inner layer of the envelope is well known to be highly homologous to the zona pellucida in mammalian eggs. In mammals, Xenopus and some teleosts, major glycoprotein components of the zona pellucida or egg envelope, are synthesized by the oocyte (Wassarman, 1988;Hedrick, 1996;Anderson, 1967;Begovac and Wallence, 1989;Chang et al, 1996Chang et al, , 1997, however, in several other teleost species these components have been found to be synthesized by the liver (Hamazaki et al, 1984;Yamagmi et al, 1994;Fujita et al, 1998;Shimizu et al, 1998). In the latter teleosts, the egg envelope components are generally observed in females.…”

Section: Introductionmentioning

confidence: 77%

Expression and Localization of Eel Testicular ZP-homologues in Female Japanese Eels (Anguilla japonica)

Kudo

Miura

et al. 2000

Zoological Science

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Section: Introductionmentioning

confidence: 77%

Expression and Localization of Eel Testicular ZP-homologues in Female Japanese Eels (Anguilla japonica)

Kudo

Miura

et al. 2000

Zoological Science

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“…Similar to CaP, ZP transcript levels in zebrafish are abundantly transcribed during oogenesis, where 10.3% of total transcripts expressed in the ovary code for proteins of the ZPA-ZPC families (Zeng and Gong, 2002). Consistent with this, Chang et al (1996Chang et al ( , 1997 used Northern blot analysis and found carp ZP2 and ZP3 mRNAs in all stages of oocytes, with decreasing levels from early to late stage. In mouse, the expression of all three ZP genes is precisely regulated during oogenesis and restricted to a 2-week growth phase, when they represent together approximately 1.5% of the total polyA + RNA.…”

Section: Discussionmentioning

confidence: 99%

Expression pattern of the zona pellucida 3 (ZP3) gene during ovarian development and the location of ZP3 protein in oocytes in a natural, wild triploid crucian carp mutant, Carassius auratus var. Pingxiangnensis

Shi¹,

Sheng²,

Peng³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Carassius auratus var. Pingxiangnensis (designated CaP), distributed in the Pingxiang region of Jiangxi Province, China, is a natural, wild triploid crucian carp mutant that has two reproductive development modes: gynogenesis and bisexual reproduction. Little information is available about the expression pattern of the zona pellucida 3 (ZP3) gene during ovarian development and the location of the ZP3 protein in oocytes of this fish. In this study, we obtained the full-length cDNA of ZP3 (CaP_ ZP3). CaP_ZP3 contains an open reading frame of 1305 bp that encodes 435 amino acid residues. Real-time polymerase chain reaction (PCR) was used to determine the CaP_ZP3 mRNA expression levels in the ovary at different stages of maturation. Results revealed high levels of CaP_ZP3 expression in 4-to 8-month-old ovaries (stage II-stage III), with a significant decline in 9-to 12-month-old ovaries (stages IV-stage V). The high levels of CaP_ ZP3 transcripts during the early growth period suggest an important role for CaP_ZP3 in early oocyte development. In addition, a polyclonal antibody was prepared against CaP_ZP3, and the immunofluorescence localization was determined. CaP_ZP3 protein was detected close to the oocyte plasma membrane. The results also showed that no fluorescent signal was detected in stage I and II oocytes. CaP_ZP3 protein is primarily detected in stage III oocytes, and the protein accumulates as oocytes develop into stage IV oocytes. These results suggested that the transcription and translation of the CaP_ZP3 gene is asynchronous and that the transcription of the CaP_ZP3 protein occurs prior to its translation in this triploid fish.

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“…In addition to such liver-derived egg envelope-related genes, the presence of egg envelope-related genes exclusively expressed in the ovary was also found. In carp [109,110] and zebrafish [111], the expression of ZP2 and ZP3 mRNA was only observed in the ovary, and based on deduced amino acid sequences, ZP2 and ZP3 reportedly have high homology to mammalian ZPB and ZPC, respectively. However, none of the liver-derived egg envelope-related genes mentioned above have been found in Cyprinidae.…”

Section: Choriogenesis and Choriogeninmentioning

confidence: 99%