Molecular comparison of Slc11a1 and Slc11a2 genes of swamp‐ and riverine‐type water buffaloes

Abstract

Solute-linked carrier 11a and 11a2 (Slc) have been associated with disease resistance and/or susceptibility across animal species. These genes have an important mechanism in the regulation against intracellular infection. This study analysed the genetic characteristic of Slc 11a and 11a2 in swamp-type and riverine-type water buffaloes to understand their immunological distinction. Characterization of Slc11a1 and Slc11a2 genes from swamp- and riverine-type water buffaloes was carried out by molecular cloning, sequencing and phylogenetic analysis. The cloned cDNA of Slc11a1 and Slc11a2 contained an open reading frame of 1647 and 1723 nucleotides, encoding 549 and 574 amino acids, respectively. Nucleotide sequence homology of both Slc11a1 and Slc11a2 had 99% in swamp and riverine type, which gives almost identical polypeptide. However, Slc11a1 and Slc11a2 have substitutions of 5 and 1 amino acid residues, correspondingly. These substitutions suggest as a potential gene markers for resistance and/or susceptibility to intracellular infection. Furthermore, phylogenetic analysis confirmed the degree of relationship between the bubaline species and justifies the distinctness of each breed by the bootstrap value generated.

Identification of Susceptibility Genes to Allergic Rhinitis by Gene Expression Data Sets

“…concluded that SLC5A5 is a direct target gene of the p53 signaling pathway and suggested that the DNA‐damaging agents‐mediated SLC5A5 could hypothetically potentiate its upregulation in vivo . Solute‐linked carrier has been linked to disease resistance and susceptibility in animals, with research proposing its potential as an essential mechanism in the mediation against intracellular infection . Those findings were largely inconsistent with the findings of the current study, whereby these five genes were all markedly elevated in AR, and displayed sustained high levels following allergen challenge.…”

“…Solute-linked carrier has been linked to disease resistance and susceptibility in animals, with research proposing its potential as an essential mechanism in the mediation against intracellular infection. 32 Those findings were largely inconsistent with the findings of the current study, whereby these five genes were all markedly elevated in AR, and displayed sustained high levels following allergen challenge. AA metabolism has been shown to trigger a large release of a number of inflammatory regulators, including leukotrienes and prostaglandins, which may be involved in numerous inflammatory-associated diseases, such as asthma, rheumatoid arthritis, as well as various types of cancers.…”

Immunogenetic Predictors of Allergic Rhinitis

“…SLC5A5 є прямим геном-мішенню сигнального шляху p53, опосередкований агентами, що пошкоджують ДНК [26,27]. Він пов'язаний із стійкістю до хвороб та має потенціал важливого механізму посередництва проти внутрішньоклітинної інфекції [28].…”