Molecular Composition of Mitochondrial ATP-sensitive Potassium Channels Probed by Viral Kir Gene Transfer

Seharaseyon, Jegatheesan; Ohler, Andreas; Sasaki, Norihito; Fraser, Heather; Sato, Toshiaki; Johns, David C.; O’Rourke, Brian; Marbán, Eduardo

doi:10.1006/jmcc.2000.1226

Cited by 88 publications

(58 citation statements)

References 29 publications

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“…Several studies have been reported since the initial report by Liu et al on the pharmacology and regulation of mitoK ATP using the flavoprotein oxidation technique [6,[28][29][30][31][32]. These studies support the previous results from isolated mitochondria as far as the selectivity of K + channel modulators for the mitoK ATP vs. the surface isoform.…”

Section: Studies On Intact Myocytessupporting

confidence: 79%

Mitochondrial K channels in cell survival and death

Ardehali¹,

O’Rourke²

2005

Journal of Molecular and Cellular Cardiology

196

151

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Since the discovery of the mitochondrial ATP-sensitive potassium channel (mitoK ATP ) more than 13 years ago, it has been implicated in the processes of ischemic preconditioning (IPC), apoptosis and mitochondrial matrix swelling. Different approaches have been employed to characterize the pharmacological profile of the channel, and these studies strongly suggest that cellular protection well correlates with the opening of mitoK ATP . However, there are many questions regarding mitoK ATP that remain to be answered. These include the very existence of mitoK ATP itself, its degree of importance in the process of IPC, its response to different pharmacological agents, and how its activation leads to the process of IPC and protection against cell death. Recent findings suggest that mitoK ATP may be a complex of multiple mitochondrial proteins, including some which have been suggested to be components of the mitochondrial permeability transition pore. However, the identity of the pore-forming unit of the channel and the details of the interactions between these proteins remain unclear. In this review, we attempt to highlight the recent advances in the physiological role of mitoK ATP and discuss the controversies and unanswered questions.

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Section: Studies On Intact Myocytessupporting

confidence: 79%

Mitochondrial K channels in cell survival and death

Ardehali¹,

O’Rourke²

2005

Journal of Molecular and Cellular Cardiology

196

151

View full text Add to dashboard Cite

show abstract

“…Na v 1.5 on IDs was similar to those fixed with FA reported in earlier studies [8]. The heteromultimeric complexes of sulfonylurea receptors (SUR) and K + inward rectifier (K ir ) gene products were suggested to form sarcolemmal channels [11]; however, the subcellular localisation of K ir has rarely been found. In this study, K ir 6.2 was only detected as possessing a transversely striated pattern.…”

Section: Resultssupporting

confidence: 82%

Comparison of formaldehyde and methanol fixatives used in the detection of ion channel proteins in isolated rat ventricular myocytes by immunofluorescence labelling and confocal microscopy

et al. 2015

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“…The uncertainty as to whether Kir6.1 is a subunit of the sarcolemmal (Seharaseyon et al 2000;Kuniyasu et al 2003) or mitochondrial K ATP channel (Lacza et al 2003;Singh et al 2003;Zhou et al 2005) applies to SURs as well. Originally, the molecular mass of SUR was determined to be 140-170 kDa by gel filtration chromatography and about z250 kDa by radiation inactivation analysis (Skeer et al 1994), suggesting that the b-cell SUR is composed of more than one subunit in the native membrane.…”

Section: Discussionmentioning

confidence: 99%

“…Cardiomyocyte mitochondria contain both Kir6.1 and Kir6.2 (Lacza et al 2003;Singh et al 2003;Zhou et al 2005), although conflicting views exist (Seharaseyon et al 2000;Kuniyasu et al 2003). How do SURs cooperate with these pore-forming subunits in mitochondria?…”

mentioning

confidence: 99%

Localization of Sulfonylurea Receptor Subunits, SUR2A and SUR2B, in Rat Heart

Zhou

Suzuki

et al. 2007

J Histochem Cytochem.

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(KK) S U M M A R Y To understand the possible functions and subcellular localizations of sulfonylurea receptors (SURs) in cardiac muscle, polyclonal anti-SUR2A and anti-SUR2B antisera were raised. Immunoblots revealed both SUR2A and SUR2B expression in mitochondrial fractions of rat heart and other cellular fractions such as microsomes and cell membranes. Immunostaining detected ubiquitous expression of both SUR2A and SUR2B in rat heart in the atria, ventricles, interatrial and interventricular septa, and smooth muscles and endothelia of the coronary arteries. Electron microscopy revealed SUR2A immunoreactivity in the cell membrane, endoplasmic reticulum (ER), and mitochondria. SUR2B immunoreactivity was mainly localized in the mitochondria as well as in the ER and cell membrane. Thus, SUR2A and SUR2B are not only the regulatory subunits of sarcolemmal K ATP channels but may also function as regulatory subunits in mitochondrial K ATP channels and play important roles in cardioprotection. (J Histochem Cytochem 55:795-804, 2007)

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Molecular Composition of Mitochondrial ATP-sensitive Potassium Channels Probed by Viral Kir Gene Transfer

Cited by 88 publications

References 29 publications

Mitochondrial K channels in cell survival and death

Mitochondrial K channels in cell survival and death

Comparison of formaldehyde and methanol fixatives used in the detection of ion channel proteins in isolated rat ventricular myocytes by immunofluorescence labelling and confocal microscopy

Localization of Sulfonylurea Receptor Subunits, SUR2A and SUR2B, in Rat Heart

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