Molecular Confirmation of Massive Taenia pisiformis Cysticercosis in One Rabbit in Poland

Samorek-Pieróg, Małgorzata; Karamon, Jacek; Brzana, Adam; Bilska–Zając, Ewa; Zdybel, Jolanta; Cencek, Tomasz

doi:10.3390/pathogens10081029

Cited by 8 publications

(3 citation statements)

References 34 publications

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“…The second PCR employed in the present study amplified a partial sequence of cytochrome c oxidase subunit 1 ( cox1 fragment gene) using a protocol designed by Bowles et al [ 44 ] and modified by Casulli et al [ 45 ], which has been described previously [ 43 ] and is reported in Table 1 . The PCR amplified a fragment to about 446 bp using primers CO1F and CO1R ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland

Samorek-Pieróg

Karamon

Brzana³

et al. 2022

Pathogens

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(1) Background: Taenia crassiceps is a cosmopolitan tapeworm endemic to the northern hemisphere with an indirect lifecycle. Its definitive hosts are carnivores, and its intermediate hosts are rodents and rabbits. Nonhuman primates in zoos appear to be highly susceptible to T. crassiceps cysticercosis. The aim of this study was to confirm the presence and the molecular characterization of T. crassiceps cysts isolated from a captive ring-tailed lemur. (2) Methods: Surgery revealed multifocal, transparent saccules containing several thin-walled tapeworm cysticerci. In some of the metacestodes, single or multiple exogenous buds from daughter cysticerci were spotted. A molecular analysis was performed to confirm our morphological examinations, using two protocols to obtain the partial nad1 and cox1 genes of the Taenia sp. (3) Results: On the basis of morphological features and molecular analysis, the cysticerci were identified as T. crassiceps metacestodes, and products taken from the PCRs were sequenced. With respect to interpreting the sequencing results of the obtained amplicons, we compared them with data in the GenBank database, proving that, in this case, the causative agent was indeed T. crassiceps. (4) Conclusions: The received data can be used to supplement descriptions of this species. To the best of our knowledge, this is the first case of cysticercosis caused by T. crassiceps in a nonhuman primate in Poland.

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Section: Methodsmentioning

confidence: 99%

Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland

Samorek-Pieróg

Karamon

Brzana³

et al. 2022

Pathogens

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“…Подібні дослідження були проведені у Польщі. Молекулярно підтверджено метацестоду T. Pisiformis у кроликів (Samorek-Pieróg et al, 2021). Лікування личинкової стадії C. pisiformis цестоди T. pisiformis не розроблене.…”

Section: поширення діагностика та лікування гельмінтозних хвороб кролівunclassified

Parasitic diseases of rabbits (distribution, diagnosis and treatment)

Kruchynenko

2023

SMLNUVMB

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This review presents data on the most common causative agents of protozooses, helminthiases, and ectoparasitic diseases of rabbits (Oryctolagus cuniculus, Linnaeus, 1758), which are of interest to veterinarians. Modern methods of diagnosing parasitic diseases in rabbits have been determined. Chemotherapeutic drugs available on the world pharmaceutical market for therapeutic and preventive treatments in rabbits are listed. The main causative agents of protozoan, helminthic, and ectoparasitic diseases of rabbits are considered, considering the modern systematic position of parasites. The features of the distribution of parasitic diseases in Ukraine and the world, according to the data of well-known scientists in the field of veterinary parasitology, are briefly given. Information on the specifics of the use of antiparasitic drugs in the case of animals affected by protozooses, helminths, and ticks is summarized. The work briefly provides information on the most common medicines and chemical combinations that are the active ingredients of these drugs (their chemical names existing in different countries, synonyms, and main pharmacological properties). Information on the dosage and features of the use of antiparasitic agents in the case of rabbit diseases is given following the data of modern scientific literature and the relevant recommendations for their industrial use. During the inspection, it was established that rabbits are most often affected by eimeriosis (coccidiosis). Currently, 15 species of Eimeria spp. have been identified. Young animals (2–6 months) are most susceptible to eimeriosis. The peak of infestation in the territory of Ukraine falls in the spring-summer period. It was found that P. ambiguus is most often detected among helminthiases in rabbits in our country. Rabbits aged 1 to 2 years are most susceptible to these helminths, and the peak of infestation occurs in January. In rabbits, psoroptosis is one of the most common acaroses, the causative agent of which is ticks of the P. cuniculi species. The purpose of this work was to show the current state of rabbits' most common parasitic diseases on the territory of Ukraine and in the world in general, highlight the existing antiparasitic drugs, and summarize information on their use. It has been established that animals with encephalitozoonosis are the most difficult to diagnose and treat. At the same time, there is a wide range of coccidiostats and coccidiolytics for treating rabbits affected by eimeries. For the infestation of animals with helminths, it is appropriate to use benzimidazoles and ivermectins. In the case of acaroses, ivermectin and fungicidal-acaricidal ointments remain effective drugs. The analysis of literary sources will allow for the expansion of the already available data on the spread, prevention, and treatment of parasitic diseases in rabbits. The given information will help ensure the rabbits' veterinary well-being on Ukraine's territory.

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“…Cysticercus pisiformis is a widely prevalent larval form of tapeworm that primarily parasitizes the liver, omentum, and mesenteries of lagomorphs and rodents, leading to the development of cysticercosis pisiformis ( 1 , 2 ). Among these hosts, rabbits are the most frequently affected and suffer significant consequences from cysticercosis pisiformis.…”

Section: Introductionmentioning

confidence: 99%

Cysticercus pisiformis-derived novel-miR1 targets TLR2 to inhibit the immune response in rabbits

Chen¹,

Pu²,

Wang³

et al. 2023

Front. Immunol.

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Cysticercosis pisiformis, a highly prevalent parasitic disease worldwide, causes significant economic losses in the rabbit breeding industry. Previous investigations have identified a novel microRNA, designated as novel-miR1, within the serum of rabbit infected with Cysticercus pisiformis. In the present study, we found that C. pisiformis-derived novel-miR1 was released into the rabbit serum via exosomes. Through computational analysis using TargetScan, miRanda, and PITA, a total of 634 target genes of novel-miR1 were predicted. To elucidate the functional role of novel-miR1, a dual-luciferase reporter assay was utilized and demonstrated that novel-miR1 targets rabbit Toll-like receptor 2 (TLR2). Rabbit peripheral blood lymphocytes (PBLCs) were transfected with novel-miR1 mimic and mimic NC, and the in vitro experiments confirmed that novel-miR1 suppressed the expression of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 through the nuclear factor kappa B (NF-κB) pathway. In vivo experiments demonstrated that novel-miR1 was significantly upregulated during the 1–3 months following infection with C. pisiformis in rabbits. Notably, this upregulation coincided with a downregulation of TLR2, P65, pP65, TNF-α, IL-1β, and IL-6 in PBLCs. Collectively, these results indicate that the novel-miR1 derived from C. pisiformis inhibited the rabbits’ immune response by suppressing the NF-κB-mediated immune response. This immune modulation facilitates parasite invasion, survival, and establishment of a persistent infection.

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Molecular Confirmation of Massive Taenia pisiformis Cysticercosis in One Rabbit in Poland

Cited by 8 publications

References 34 publications

Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland

Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland

Parasitic diseases of rabbits (distribution, diagnosis and treatment)

Cysticercus pisiformis-derived novel-miR1 targets TLR2 to inhibit the immune response in rabbits

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