Molecular control of rodent spermatogenesis

Jan, Sabrina Z.; Hamer, Geert; Repping, Sjoerd; Rooij, Dirk G. de; Pelt, Ans M.M. van; Vormer, Tinke L.

doi:10.1016/j.bbadis.2012.02.008

Cited by 177 publications

(158 citation statements)

References 207 publications

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“…At E7.5, these sexually undifferentiated PGCs start migrating toward the genital ridge, involving the interactions of the membrane receptors KIT (c-Kit/CD117) and CXCR4 with their respective ligands SCF and SDF1, where they will become resident and enclosed by Sertoli cells, forming testicular cords at E12.5dpc in rodents (reviewed in Jan et al (2012)). During their migration, PGCs undergo genome-wide DNA demethylation, leading to the erasure of parental imprints (Reik et al 2001, Seki et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Mammalian gonocyte and spermatogonia differentiation: recent advances and remaining challenges

Manku¹,

Culty²

2015

Reproduction

127

105

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The production of spermatozoa relies on a pool of spermatogonial stem cells (SSCs), formed in infancy from the differentiation of their precursor cells, the gonocytes. Throughout adult life, SSCs will either self-renew or differentiate, in order to maintain a stem cell reserve while providing cells to the spermatogenic cycle. By contrast, gonocytes represent a transient and finite phase of development leading to the formation of SSCs or spermatogonia of the first spermatogenic wave. Gonocyte development involves phases of quiescence, cell proliferation, migration, and differentiation. Spermatogonia, on the other hand, remain located at the basement membrane of the seminiferous tubules throughout their successive phases of proliferation and differentiation. Apoptosis is an integral part of both developmental phases, allowing for the removal of defective cells and the maintenance of proper germ-Sertoli cell ratios. While gonocytes and spermatogonia mitosis are regulated by distinct factors, they both undergo differentiation in response to retinoic acid. In contrast to postpubertal spermatogenesis, the early steps of germ cell development have only recently attracted attention, unveiling genes and pathways regulating SSC self-renewal and proliferation. Yet, less is known on the mechanisms regulating differentiation. The processes leading from gonocytes to spermatogonia have been seldom investigated. While the formation of abnormal gonocytes or SSCs could lead to infertility, defective gonocyte differentiation might be at the origin of testicular germ cell tumors. Thus, it is important to better understand the molecular mechanisms regulating these processes. This review summarizes and compares the present knowledge on the mechanisms regulating mammalian gonocyte and spermatogonial differentiation.

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Section: Introductionmentioning

confidence: 99%

Mammalian gonocyte and spermatogonia differentiation: recent advances and remaining challenges

Manku¹,

Culty²

2015

Reproduction

127

105

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“…During development, germ cells undergo a series of specialized cellular processes, including migration, sexual differentiation, and meiosis, and mature male and female gametes have few cellular features in common (1,2). Nevertheless, germ cells of both sexes and all life stages are unified by the shared ability to contribute genetic material to the next generation: unlike any other cell type, terminally differentiated germ cells can give rise to a totipotent zygote.…”

mentioning

confidence: 99%

A set of genes critical to development is epigenetically poised in mouse germ cells from fetal stages through completion of meiosis

Lesch

Dokshin

Young

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

150

142

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Significance Germ cells, meaning the mature sperm and egg and their developmental precursors, carry the DNA that is passed from one generation to the next. Although the sperm and egg are highly specialized, differentiated cells, they meet at fertilization to produce a totipotent zygote, a cell that can generate any other cell type. We report the finding that a set of developmentally important genes is kept in a “poised” state in the germ cells: although these genes are never expressed in the germ cells themselves, they maintain a chromatin state usually associated with the potential for rapid gene activation. We propose that maintenance of this poised state in the germ cells contributes to the generation of totipotency in the fertilized zygote.

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“…Причинами нарушения сперматогенеза могут быть де-фектная миграция зародышевых клеток, гибель сперма-тогониальных стволовых клеток, нарушения в структуре СК в профазе I мейоза, дефектный спермиогенез или нарушение функций микроокружения [39]. Фрагмента-ция СК может быть следствием апоптоза или некроза мейотических клеток, а высокие уровни (20-70 %) -следствием развития блока мейоза на стадии сперма-тоцитов I порядка [40].…”

Section: Discussionunclassified

Genotoxic effects of N-nitrosodimethylamine in somatic and generative cells of mice

Lovinskaya

Владимировна²,

Kolumbayeva

et al. 2017

Ecological genetics

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N-Nitrosodimethylamine (NDMA) was shown to have genotoxic properties in acute and subacute studies on laboratory mice. The organ-specificity of the genotoxic effect of NDMA was revealed using the Comet assay. The most sensitive organs to the action of NDMA were kidneys and liver. DNA damage in liver cells of NDMA-treated animals at doses of 4.0 and 8.0 mg/kg, increased compared to control in 6.9 and 12.5 (р < 0.001), and in kidney cells – in 8.1 and 14.2 times (р < 0.001), respectively. NDMA also showed genotoxic activity in the reproductive cells of experimental animals, causing structural disorders of synaptonemal complexes in spermatocyte. In NDMA-treated animals at a dose of 2.0 mg/kg in acute and subacute studies, the level of spermatocytes with damaged synaptonemal complexes increased statistically significantly compared to control in 6.0 and 7.0 (р < 0.05) times, respectively.

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Molecular control of rodent spermatogenesis

Cited by 177 publications

References 207 publications

Mammalian gonocyte and spermatogonia differentiation: recent advances and remaining challenges

Mammalian gonocyte and spermatogonia differentiation: recent advances and remaining challenges

A set of genes critical to development is epigenetically poised in mouse germ cells from fetal stages through completion of meiosis

Genotoxic effects of N-nitrosodimethylamine in somatic and generative cells of mice

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