Molecular criteria for screening of Beauveria strains used for insect pest control

Lednev, G. R.; Tokarev, Yurij; Uspanov, A. M.; Malysh, Jylia; Duisembekov, B. A.; Sabitova, Marina; Levchenko, M. V.; Smagulova, Sholpan; Orazova, S. B.; Amanov, Serzhan; Sagitov, A. O.

doi:10.1016/j.jbiotec.2014.07.215

Cited by 8 publications

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“…Our findings were consistent with these reports as the major species in the forest ecosystems were B. pseudobassiana , and the proportion of B. bassiana increased towards the southern (dfb) more agricultural areas ( Figure 2 B and Figure S3 ). Other studies have also indicated that B. bassiana is associated with the steppe zone, whereas B. pseudobassiana is more common in forest ecosystems [ 70 ]. Although only 10 B. bassiana isolates were obtained during the survey, they were collected from similar spectra of insect orders to B. pseudobassiana .…”

Section: Discussionmentioning

confidence: 99%

Distribution and Diversity of Beauveria in Boreal Forests of Northern European Russia

Казарцев

Lednev

2021

Microorganisms

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The distribution and genetic diversity of 91 of Beauveria isolates collected during a long-term survey in boreal forests of northern European Russia was studied. Based on morphological and sequence analysis of TEF and Bloc loci, three Beauveria spp. were identified: B. pseudobassiana, B. bassiana, and B. caledonica, with abundance of 81, 11, and 8%, respectively. Through multilocus sequencing, four haplotypes of B. bassiana and two haplotypes of B. caledonica were detected. Twelve haplotypes of B. pseudobassiana with non-random distribution were identified. Two haplotypes of B. pseudobassiana were the most abundant and widespread occurring across the whole study area, whereas others tended to be more specific to either the north or south of the study area, indicating the presence of different subpopulations. For further analysis of these putative subpopulations, southern and northern areas were separated along the boundary of the Köppen–Geiger climate zones (dfb and dfc), and the genetic structure was examined by analysis of molecular variance and spatial autocorrelation. Molecular evidence of intraspecific recombination of B. pseudobassiana and B. bassiana across northern European Russia area was indicated.

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Section: Discussionmentioning

confidence: 99%

Distribution and Diversity of Beauveria in Boreal Forests of Northern European Russia

Казарцев

Lednev

2021

Microorganisms

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“…Prior to other manipulations, the samples were consequently surface-sterilized with 1 % solution of sodium hypochlorite and 70 % ethanol, followed by triple washing with sterile distilled water. After observation of mycelium growth, fungi were routinely isolated into pure culture and identified using light microscopy and molecular genetic analysis [11]. The model insect hosts were laboratory cultures of migratory locust Locusta migratoria migratorioides and desert locust Shistocerca gregaria from the collection of Moscow Zoo, reared in cages at 32-34°C and fed on wheat.…”

Section: Methodsmentioning

confidence: 99%

The effect of endophytic colonization of wheat plants by the fungus Beauveria bassiana on the development of the nymphs of the migratory and desert locusts

Levchenko¹,

Gerus²,

Malysh³

et al. 2020

BIO Web Conf.

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The experiments were aimed at evaluation of the effect of feeding with wheat colonized by the fungus Beauveria bassiana on the development of locusts. The seeds were treated with fungal conidia and 90 days after sowing, the plant parts were fed to II instar nymphs of laboratory colonies of migratory and desert locusts within the period of 18 days. The mortality levels between experimental and control (fed with untreated wheat) groups were not significantly different though a tendency of an increase in variants with contaminated fodder was observed. Two weeks after the beginning of the bioassay, insect mortality was 20-30% and 15% in control. As many as 50% of migratory locust and 40% of desert locust cadavers were covered by a fungal mycelium. Sequencing of two diagnostic DNA loci has confirmed attribution of the reisolate to the genotype of the initial Beauveria bassiana strain BBK-1. It can be therefore concluded that the endophyte fungus is capable of causing classical pathogenesis of mycosis in locust nymphs. Moreover, the nymphal development was retarded by feeding with the fungus-colonized plants.

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“…have been developed by All-Russian Institute of Plant Protection in collaboration with colleagues from Kazakhstan. These formulations have high efficiency in arid zones [30].…”

Section: Fungimentioning

confidence: 99%

Entomopathogenic microorganisms in locusts and grasshoppers populations and prospects for their use for control of this pest group

2020

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Brief information on the main groups of microorganisms parasitizing on locusts and grasshoppers is given. It has been shown that viral and most bacterial infections have no practical prospects, both as natural regulators of density populations and as potential producers of bioinsecticides. Alpha-proteobacteria, entomophthoralean fungi and microsporidia may make some contributions as natural mechanisms of population dynamics. The most significant group as producers of biopesticides are anamorphic ascomycetes from the genera Metarhizium and Beauveria.

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Molecular criteria for screening of Beauveria strains used for insect pest control

Cited by 8 publications

References 0 publications

Distribution and Diversity of Beauveria in Boreal Forests of Northern European Russia

Distribution and Diversity of Beauveria in Boreal Forests of Northern European Russia

The effect of endophytic colonization of wheat plants by the fungus Beauveria bassiana on the development of the nymphs of the migratory and desert locusts

Entomopathogenic microorganisms in locusts and grasshoppers populations and prospects for their use for control of this pest group

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