Molecular Crowding and Viscosity as Determinants of Translational Diffusion of Metabolites in Subcellular Organelles

García-Pérez, Ana Isabel; López-Beltrán, Emilio A.; Klüner, Peter; Luque, J.; Ballesteros, Paloma; Cerdán, Sebastián

doi:10.1006/abbi.1998.1051

Cited by 108 publications

(77 citation statements)

References 28 publications

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“…They reported that medulloblastoma with a low ADC value was observed despite the low cell density, in this medulloblastoma, the nuclear / cytoplasm ratio was high. A similar phenomenon is reported by Garcia -Perez et al 19) . It is assumed that the reduction of ADC is not a phenomenon reflecting only simple cell density but actually consists of a combination of multiple phenomena.…”

Section: Bsupporting

confidence: 89%

Apparent diffusion coefficient on magnetic resonance imaging (MRI) in bladder cancer: relations with recurrence/progression risk

Kikuchi

Shigihara

Hashimoto

et al. 2017

FJMS

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AIMS :To evaluate the relationship between the apparent diffusion coefficient (ADC) value for bladder cancer and the recurrence/progression risk of post -transurethral resection (TUR). METHODS : Forty -one patients with initial and non -muscle -invasive bladder cancer underwent MRI from 2009 to 2012. Two radiologists measured ADC values. A pathologist calculated the recurrence/progression scores, and risk was classified based on the scores. Pearson's correlation was used to analyze the correlations of ADC value with each score and with each risk group, and the optimal cut -off value was established based on receiver operating characteristic (ROC) curve analysis. Furthermore, the relationship between actual recurrence / progression of cases and ADC values was examined by Unpaird U test. RESULTS : There were significant correlations between ADC value and the recurrence score as well as the progression score (P<0.01, P<0.01, respectively). There were also significant correlations between ADC value and the recurrence risk group as well as progression risk group (P=0.042, P<0.01, respectively). The ADC cut -off value on ROC analysis was 1.365 (sensitivity 100% ; specificity 97.4%) for the low and intermediate recurrence risk groups, 1.024 (sensitivity 47.4% ; specificity 100%) for the intermediate and high recurrence risk groups, 1.252 (sensitivity 83.3% ; specificity 81.3%) for the low and intermediate progression risk groups, and 0.955 (sensitivity 87.5% ; specificity 63.2%) between the intermediate and high progression risk groups. The difference between the ADC values of the recurrence and nonrecurrence group in Unpaired t test was significant (P<0.05). CONCLUSION : ADC on MRI in bladder cancer could potentially be useful, non -invasive measurement for estimating the risks of recurrence and progression.

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Section: Bsupporting

confidence: 89%

Apparent diffusion coefficient on magnetic resonance imaging (MRI) in bladder cancer: relations with recurrence/progression risk

Kikuchi

Shigihara

Hashimoto

et al. 2017

FJMS

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“…To establish a parallelism between intracellular T 2 and ADC values and water dynamics in polyol media those parameters were analyzed in solutions of PEG and glycerol prepared in culture medium. 19,20 As Figure 3B,C shows, T 2 values decreased in glycerol solutions higher than 5% (w : w), whereas PEG was more effective with ADC values.…”

Section: No Induces Apoptosis In Raw 2647 Cellsmentioning

confidence: 84%

Intracellular water motion decreases in apoptotic macrophages after caspase activation

et al. 2001

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Triggering of the macrophage cell line RAW 264.7 with lipopolysaccharide and interferon-g promoted apoptosis that was prevented by inhibitors of type 2 nitric oxide synthase or caspase. Using 1 H NMR analysis, we have investigated the changes of the intracellular transverse relaxation time (T 2 ) and apparent diffusion coefficient (ADC) as parameters reflecting the rotational and translational motions of water in apoptotic macrophages. T 2 values decreased significantly from 287 to 182 ms in cells treated for 18 h with NO-donors. These changes of T 2 were prevented by caspase inhibitors and were not due to mitochondrial depolarization or microtubule depolymerization. The decrease of the intracellular values of T 2 and ADC in apoptotic macrophages was observed after caspase activation, but preceded phosphatidylserine exposure and nucleosomal DNA cleavage. The changes of water motion were accompanied by an enhancement of the hydrophobic properties of the intracellular milieu, as detected by fluorescent probes. These results indicate the occurrence of an alteration in the physicochemical properties of intracellular water during the course of apoptosis.

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“…28 The assessment of diffusion anisotropy in tumor tissue is a complicated issue, which is affected by the ratio of extracellular to intracellular space, vascularity, edema, microcysts, and extracellular matrix. 25,[29][30][31][32] We speculate that the complexity of diffusion anisotropy determination renders FA to be a less specific and less accurate tensor metric than ADC in distinguishing lymphomas from GBMs.…”

Section: Discussionmentioning

confidence: 99%

Primary Cerebral Lymphoma and Glioblastoma Multiforme: Differences in Diffusion Characteristics Evaluated with Diffusion Tensor Imaging

Toh¹,

Castillo²,

Wong³

et al. 2007

AJNR Am J Neuroradiol

212

161

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Molecular Crowding and Viscosity as Determinants of Translational Diffusion of Metabolites in Subcellular Organelles

Cited by 108 publications

References 28 publications

Apparent diffusion coefficient on magnetic resonance imaging (MRI) in bladder cancer: relations with recurrence/progression risk

Apparent diffusion coefficient on magnetic resonance imaging (MRI) in bladder cancer: relations with recurrence/progression risk

Intracellular water motion decreases in apoptotic macrophages after caspase activation

Primary Cerebral Lymphoma and Glioblastoma Multiforme: Differences in Diffusion Characteristics Evaluated with Diffusion Tensor Imaging

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