Molecular cytogenetic analysis in seven species of Lachenalia (Liliaceae) with the chromosome numbers of 2n=18, 22, 23, 26 and 28 by DAPI staining and FISH using 5S rDNA and 18S rDNA probes

Hamatani, Shuichi; Tagashira, Norikazu; Kondo, Katsuhiko

doi:10.3199/iscb.5.55

Cited by 3 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, L. longituba, which have same chromosome number and diploid (2n=28, x=14) as L. congesta included in the first group (Hamatani 2011). Because L. longituba also did not have clear DAPIpositive bands, however had two FISH signals of 5S rDNA sites on distal position of the long arms of small chromosomes (Hamatani et al 2010). Thus, these FISH data suggested that despite L. congesta and L. longituba had common chromosome feature (2n=28, x=14) and this is unique character among Lachenalia, they are belong to another group.…”

Section: Resultsmentioning

confidence: 85%

“…Nine species and three varieties of Lachenalia had the chromosome number of 2n=28 (Spies 2004, Hamatani 2011. Among them, seven species and three varieties confirmed as tetraploid (X=7), whereas L. longituba could be identified as a diploid (X=14) because this species had 14 pairs of chromosomes characterized by their individual features, and had two FISH signals of 5S rDNA sites and 18S rDNA sites, respectively (Hamatani et al 2010).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular cytogenetical and phylogenetical studies of Lachenalia congesta (Asparagaceae)

Hamatani¹,

Masuda

Uchida

et al. 2012

Chromosome Botany

Self Cite

View full text Add to dashboard Cite

ABSTRACT. The ploidy level and the phylogenic position of Lachenalia congesta W. F. Barker (Asparagaceae) were analyzed by karyomorphological, molecular cytogenetical and molecular phylogenetical studies. The chromosome number of L. congesta was 2n=28, and these chromosomes showed bi-modal karyotype with four large sized chromosomes and 24 small sized chromosomes. The ploidy level of this species was diploid, because each two chromosomes made 14 pairs on the karyotype analysis by acetoorcein staining, DAPI staining and FISH methods. In addition two signals of 18S rDNA sites were detected by the FISH method supported this assumption. All 28 chromosomes of this species did not show any clear DAPI-positive bands and four signals of 5S rDNA sites were detected near the centromeres of small sized chromosomes. These characters were quite different from the characters observed by karyomorphological and molecular cytogenetical studies on common Lachenalia species with basic chromosome number of x=7 or 8. The data from molecular phylogenetic analysis of internal transcribed spacer (ITS) region supported this hypothesis. KEYWORDS: DAPI, FISH, Karyotype, Lachenalia congesta, Phylogenetic analysis, ITSChromosome Botany (2012) Lachenalia is distributed in the western part of South Africa and the southern part of Namibia, and includes ca. 110 species. This genus was formerly classified in the Hyacinthaceae or Liliaceae, although now it revised in the Asparagaceae followed to the third Angiosperm Phylogeny Group (APG III) classification (The Angiosperm Phylogeny Group 2009).Lachenalia congesta W. F. Barker is a dwarf species and is found on mountain slopes of Eastern Cape interior at up to alt. 2400 m with large colonies (Duncan 2009). Duncan (2009) described that Lachenalia congesta was a very distinctive species which cannot be easily mistaken against any other member of Lachenalia during the flowering season. It was recognized by two prostrate, broadly lanceolate (or occasionally ovate), leathery leaves that are dark olive-green on the upper surface and deep magenta below, with prominent dark maroon margins (Duncan 2009). Johnson and Brandham (1997) reported that the chromosome number of L. congesta was 2n=26, 28, although any other karyomorphological information about this species were not described. The molecular phylogenetic analysis using chlorophyll (cp) DNA data of genus Lachenalia indicated that L. congesta was closely related to six Polyxena species (these were transferred to genus Lachenalia by Manning et al. 2004) and three Lachenalia species (Spies 2004), although phylogenetic information from nuclear DNA analysis were not reported.In this report, we attempt to identify the ploidy level of L. congesta by the karyomorphological analysis and fluorescence in situ hybridization (FISH) methods, and to clarify the molecular phylogenetic relationship of this species among genus Lachenalia on the basis of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA. MATERIALS AND METHODSPlant materials Some pla...

show abstract

Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Molecular cytogenetical and phylogenetical studies of Lachenalia congesta (Asparagaceae)

Hamatani¹,

Masuda

Uchida

et al. 2012

Chromosome Botany

Self Cite

View full text Add to dashboard Cite

show abstract

“…Interspecific variation in the distribution of DAPI +ve signals [65,67] and CMA +ve signals [67] have been reported in Muscari. Varied distribution of DAPI +ve signals has also been reported in species of Lachenalia [68,69]. In Drimia (tribe Urgineeae), CMA +ve /DAPI −ve signals were associated with nucleolar chromosomes, with some interspecific variations in additional signals [37].…”

Section: Discussionmentioning

confidence: 78%

Comparative Cytogenetics and Fluorescent Chromosome Banding in Five Indian Species of Dipcadi Medik

Samanta

Jha²,

Ray

et al. 2023

Plants

View full text Add to dashboard Cite

The genus Dipcadi Medik. (Subfamily: Scilloideae) has a narrow distribution in India and several overlapping morphological traits make the genus taxonomically challenging at the species level. Cytogenetic characterization can provide additional taxonomic data and can be used to evaluate genetic diversity at the species level. We have accomplished comparative karyotype analysis and fluorescence banding patterns using 4′-6-Diamidino-2-phenylindole (DAPI) and Chromomycin A3 (CMA) in five Indian species for the first time. The karyotypes of D. concanense and D. goaense exhibited similar fluorochrome banding profiles. However, D. montanum, D. ursulae and D. erythraeum differ distinctly in their karyotypes. In all taxa, CMA+ve/DAPI−ve or DAPI0 (GC-rich) constitutive heterochromatin was located at the constriction region or terminal satellite of the nucleolar chromosome. DAPI+ve/CMA−ve or CMA0 (AT-rich) heterochromatin dominates in D. montanum, D. ursulae and D. erythraeum. However, D. erythraeum shows a distinct variation in fluorochrome banding pattern from all other species. The distribution of CMA and DAPI bands is a reflection of heterochromatin composition and variations acquired by different species. This characterization can be used to assess phylogenetic relationships in the understudied genus Dipcadi and may serve as a basis for other genomic analyses and evolutionary studies.

show abstract

Cytogenetic Diversity in Scilloideae (Asparagaceae): a Comprehensive Recollection and Exploration of Karyo-Evolutionary Trends

et al. 2022

View full text Add to dashboard Cite

Molecular cytogenetic analysis in seven species of Lachenalia (Liliaceae) with the chromosome numbers of 2n=18, 22, 23, 26 and 28 by DAPI staining and FISH using 5S rDNA and 18S rDNA probes

Cited by 3 publications

References 9 publications

Molecular cytogenetical and phylogenetical studies of Lachenalia congesta (Asparagaceae)

Molecular cytogenetical and phylogenetical studies of Lachenalia congesta (Asparagaceae)

Comparative Cytogenetics and Fluorescent Chromosome Banding in Five Indian Species of Dipcadi Medik

Cytogenetic Diversity in Scilloideae (Asparagaceae): a Comprehensive Recollection and Exploration of Karyo-Evolutionary Trends

Contact Info

Product

Resources

About