Molecular cytogenetic characterization of Thinopyrum intermedium-derived wheat germplasm specifying resistance to wheat streak mosaic virus

Chen, Q.; Friebe, Bernd; Conner, Robert L.; Laroche, André; Thomas, J. B.; Gill, Bikram S.

doi:10.1007/s001220050701

Cited by 47 publications

(26 citation statements)

References 16 publications

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“…About 3 mg of DNA were labeled per 50 ml of labeling reaction (using 5 ml of 10ϫenzyme mix) for 2 h at 16°C, and the reaction was stopped with 5 ml of 300 mM EDTA (pH 8.0) solution. Unincorporated nucleotides were removed by passing the labeling reaction through a Sephadex G-50 column (Millipore, Bedford, MA bridization procedure described by Chen et al (1998) was adopted with a minor modification of denaturing the chromosome slides with 70% Formamide in 2ϫSSC for 1.5 min at 80°C. Slides were examined using an Olympus BX51 epifluorescence microscope with red and green filter sets.…”

Section: Fishmentioning

confidence: 99%

“…Molecular cytogenetic techniques, such as fluorescence in situ hybridization (FISH) are valuable methods to characterize whole chromosomes or chromosome segments, which could give a complementary view for various levels of chromosomal evolution and relationships of species, and help to advance the understanding of the genetic basis of natural variation for the cultivated and wild potato species (Chen et al 1998, Dong et al 2000. Comparison of karyotype and nuclear DNA in diploid and tetraploid potato species provides valuable information related to the mechanisms of genome evolution in cytotaxonomic and evolutionary studies.…”

mentioning

confidence: 99%

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Karyotypic Evolution and Molecular Cytogenetic Analysis of Solanum pinnatisectum, a New Source of Resistance to Late Blight and Colorado Potato Beetle in Potato

Chen

Beasley

et al. 2006

CYTOLOGIA

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SummaryTo efficiently transfer important disease and insect resistance traits from diploid Mexican wild species Solanum pinnatisectum to tetraploid cultivated Solanum tuberosum, molecular cytogenetic and karyotypic analyses were carried out to examine the degree of chromosomal and genomic variation between these 2 species. The results demonstrated that the chromosome complement in S. pinnatisectum was predominantly metacentric and submetacentric, while S. tuberosum showed prominent subtelocentric and telocentric chromosomes. It appears that karyotype evolution from diploid to tetraploid originates through chromosome rearrangements, involving mainly deletions or multiplication at the same ploidy level. Fluorescence in situ hybridization (FISH) using 18S-5.8S-26S ribosomal RNA (rDNA) as a probe demonstrated that both diploid and tetraploid potato species had a single nucleolus organizer region (NOR) locus. However, the localization of the rDNA sites in one of the 2 accessions of S. pinnatisectum was characterized by a pair of morphologically distinct chromosomes, in which one was a submetacentric chromosome, while its homologous partner was a subtelocentric chromosome, which was very similar to that observed in tetraploid potato. This indicated that chromosome variation in structural alterations and nuclear DNA content, as well as loss or addition of highly repetitive sequences could play a role in potato evolution and development of new cultivars. This study offers a useful molecular cytogenetic marker for species identification, chromosome inheritance and potential introgression in future intergenetic hybridization experiments with Mexican wild potato species.

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Section: Fishmentioning

confidence: 99%

mentioning

confidence: 99%

Karyotypic Evolution and Molecular Cytogenetic Analysis of Solanum pinnatisectum, a New Source of Resistance to Late Blight and Colorado Potato Beetle in Potato

Chen

Beasley

et al. 2006

CYTOLOGIA

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“…The inability to distinguish between inter-and intra-genomic chromosomal pairing may also significantly complicate the analyses of pairing data. In some cases, due to the highly polyploid nature of these Thinopyrum species and the close relationship among the J, J S and S genomes and the wheat ABD genomes, it was difficult to interpret the genomic composition of these wheat-Thinopyrum partial amphiploids (Wang, 1992;Monte et al, 1993;Hsiao et al, 1995;Zhang et al, 1996a, b;Chen et al, 1997Chen et al, , 1998d.…”

Section: Utilization Of S Genomic Dna As a Probe In The Identificatiomentioning

confidence: 99%

“…In the wheat substitution line CI15092, a WSMV-resistance gene designated, Wsm1, was located on the short arm of a group 4 Th. intermedium chromosome Gill et al, 1995), which was later shown to belong to the J S genome (Chen et al, 1998d(Chen et al, , 1999a. Barley yellow dwarf virus resistance derived from Th.…”

Section: J S Genome Chromosomes and Their Linkages With Disease And Mmentioning

confidence: 99%

Detection of alien chromatin introgression from <i>Thinopyrum</i> into wheat using S genomic DNA as a probe – A landmark approach for <i>Thinopyrum</i> genome research

Chen¹

2005

Cytogenet Genome Res

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The introduction of alien genetic variation from the genus Thinopyrum through chromosome engineering into wheat is a valuable and proven technique for wheat improvement. A number of economically important traits have been transferred into wheat as single genes, chromosome arms or entire chromosomes. Successful transfers can be greatly assisted by the precise identification of alien chromatin in the recipient progenies. Chromosome identification and characterization are useful for genetic manipulation and transfer in wheat breeding following chromosome engineering. Genomic in situ hybridization (GISH) using an S genomic DNA probe from the diploid species Pseudoroegneria has proven to be a powerful diagnostic cytogenetic tool for monitoring the transfer of many promising agronomic traits from Thinopyrum. This specific S genomic probe not only allows the direct determination of the chromosome composition in wheat-Thinopyrum hybrids, but also can separate the Th. intermedium chromosomes into the J, J^S and S genomes. The J^S genome, which consists of a modified J genome chromosome distinguished by S genomic sequences of Pseudoroegneria near the centromere and telomere, carries many disease and mite resistance genes. Utilization of this S genomic probe leads to a better understanding of genomic affinities between Thinopyrum and wheat, and provides a molecular cytogenetic marker for monitoring the transfer of alien Thinopyrum agronomic traits into wheat recipient lines.

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“…Fluorescence genomic in situ hybridization (GISH) has been widely used for identifying alien chromatins in intergeneric hybrids and for determining the break-points of translocated chromosomes in Triticeae (Castilho et al, 1996;Chen et al, 1998;Fominaya et al, 1997;Francki et al, 1997;Islam-Faridi and Mujeeb-Kazi, 1995;Jiang and Gill, 1993;Lima-Brito et al, 1997;Mukai et al, 1993;Schwarzacher et al, 1989). In addition, C-banding analysis before or after GISH is effective and useful for identifying alien chromatins (Hutchinson and Seal, 1983;Jiang and Gill, 1993;Zhong et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Introduction of multi-alien chromatins carrying different powdery mildew-resistant genes from rye and Haynaldia villosa into wheat genome.

et al. 1998

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Powdery mildew-resistant wheat lines, Tra.R9002 with rye chromatin and Tra.V149 with Haynaldia villosa chromatin, were crossed to combine their different powdery mildew-resistant genes in the wheat genetic background. In the F 5 generation of Tra.R9002 × Tra.V149, a highly powdery mildew-resistant line W159 was selected according to its chromosome number 2n = 41 ~ 42. In the F 6 line W159-9 which was immune to powdery mildew, one pair of rye chromosomes and one pair of wheat-H. villosa translocated chromosomes were identified simultaneously by multicolor fluorescence genomic in situ hybridization in the wheat genetic background (chromosome number 2n = 42). Through C-banding and a sequential C-banding analysis after genomic in situ hybridization, the rye chromosomes were identified as 5R and the wheat-H. villosa translocated chromosomes were identified as 6VS-6AL. This indicates that W159-9 carries two powdery mildew-resistant genes of Pm4 on 5R and Pm21 on 6VS. The wheat chromosomes absent in W159-9 were identified as the 6AS and 5D. On the other hand, seven out of 17 progenies of another F 6 line W159-3 possessed 5R and 6VS-6AL chromosomes. These materials could be used for acquiring stable multiple powdery mildew-resistance in wheat breeding programs.

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Molecular cytogenetic characterization of Thinopyrum intermedium-derived wheat germplasm specifying resistance to wheat streak mosaic virus

Cited by 47 publications

References 16 publications

Karyotypic Evolution and Molecular Cytogenetic Analysis of Solanum pinnatisectum, a New Source of Resistance to Late Blight and Colorado Potato Beetle in Potato

Karyotypic Evolution and Molecular Cytogenetic Analysis of Solanum pinnatisectum, a New Source of Resistance to Late Blight and Colorado Potato Beetle in Potato

Detection of alien chromatin introgression from <i>Thinopyrum</i> into wheat using S genomic DNA as a probe – A landmark approach for <i>Thinopyrum</i> genome research

Introduction of multi-alien chromatins carrying different powdery mildew-resistant genes from rye and Haynaldia villosa into wheat genome.

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