Molecular cytogenetic identification of a wheat–Aegilops geniculata Roth 7Mg disomic addition line with powdery mildew resistance

Wang, Yajuan; Quan, Wei; Peng, Nana; Wang, Changyou; Yang, Xiaofei; Liu, Xinlun; Zhang, Hong; Chen, Chunhuan; Ji, Wanquan

doi:10.1007/s11032-016-0463-1

Cited by 29 publications

(36 citation statements)

References 36 publications

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“…GISH can simply determine the presence of alien chromosomes but molecular markers are valuable analysis tools for identifying the homoeology of alien chromosomes. Thus, a wheat-Aegilops geniculata 7M g addition line was identified using EST-STS markers by Wang et al [49]. In addition, Wang et al [50] identified a wheat-Leymus racemosus translocation line T5AS-7LrL·7LrS by using EST-STS markers.…”

Section: Discussionmentioning

confidence: 99%

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Liu¹,

Hou²,

Li³

et al. 2020

Agronomy

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Powdery mildew is a fungal disease that threatens wheat production throughout the world. Breeding resistant cultivars is an effective way to reduce harm caused by powdery mildew. In this study, 35 wheat-Psathyrostachys huashanica-derived lines were developed by crossing common wheat and P. huashanica Keng (2n = 2x = 14, NsNs) using embryo culture. Resistance to powdery mildew in the derived lines was identified at the seedling and adult stages. Line H5-5-4-2 was selected with immunity to powdery mildew at both growth stages. The chromosome structure of this line was characterized by cytology, genomic in situ hybridization (GISH), and expressed sequence tag-sequence-tagged site (EST-STS) analysis. The chromosome configuration was 2n = 44 = 22II. Two P. huashanica chromosomes with strong hybridization signals were detected by GISH analysis. Among 83 EST-STS markers that covered all seven homologous groups in wheat, three pairs of STS markers, BE497584, BF202643, and BG262410, located in wheat homologous group 1 amplified clear specific bands related to P. huashanica. The results indicated that resistant line H5-5-4-2 was a wheat-P. huashanica 1Ns disomic addition line.

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Section: Discussionmentioning

confidence: 99%

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Liu¹,

Hou²,

Li³

et al. 2020

Agronomy

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“…Disease resistance genes from wild relatives of wheat play important roles in control of powdery mildew. Among the 25 wheat closely related genera, 14 demonstrated immunity to powdery mildew, including Secale [24], Aegilops [25], Thinopyrum [26], and Haynaldia villosa [27]. P. huashanica is also immune to powdery mildew but wheat-P. huashanica derived lines with powdery mildew resistance have not been reported to date.…”

Section: (Continued From Previous Page)mentioning

confidence: 99%

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Zhao

Cheng

et al. 2020

BMC Plant Biol

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Background: Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) carries many outstanding agronomic traits, therefore is a valuable resource for wheat genetic improvement. Wheat-P. huashanica translocation lines are important intermediate materials for wheat breeding and studying the functions of alien chromosomes. However, powdery mildew resistance in these translocation lines has not been reported previously.Results: This study developed a novel wheat-P. huashanica translocation line TR77 by selecting a F 7 progeny from the cross between heptaploid hybrid H8911 (2n = 7x = 49, AABBDDNs) and durum wheat line Trs-372. Chromosome karyotype of 2n = 42 = 21II was observed in both mitotic and meiotic stages of TR77. Genomic in situ hybridization analysis identified two translocated chromosomes that paired normally at meiosis stage in TR77. Molecular marker analysis showed that part of chromosome 5D was replaced by part of alien chromosome fragment 5Ns. It meant replacement made part 5DL and part 5NsL·5NsS existed in wheat background, and then translocation happened between these chromosomes and wheat 3D chromosome. Fluorescence in situ hybridization demonstrated that TR77 carries dual translocations: T3DS-5NsL·5NsS and T5DL-3DS·3DL. Analysis using a 15 K-wheat-SNP chip confirmed that SNP genotypes on the 5D chromosome of TR77 matched well with these of P. huashanica, but poorly with common wheat line 7182. The translocation was physically located between 202.3 and 213.1 Mb in 5D. TR77 showed longer spikes, more kernels per spike, and much better powdery mildew resistance than its wheat parents: common wheat line 7182 and durum wheat line Trs-372.(Continued on next page)

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“…The oligonucleotide probes Oligo-pTa535 (red) and Oligo-pSc119.2 (green) (Shanghai Invitrogen Biotechnology Co. Ltd., Shanghai, China) were used for FISH and sequential FISH-GISH analysis, as described by Tang et al [25]. The results of in situ hybridization and fluorescent signals were viewed and photographed with an Olympus BX-43 microscope equipped with a DP80 camera [4].…”

Section: Gish Fish and Sequential Fish-gishmentioning

confidence: 99%

“…This is necessary to improve wheat disease resistance, enable screening of large germplasm resources, and enable the transfer and polymerization of resistance genes. It is an increasingly important approach for enriching common wheat with beneficial genes derived from related species by distant hybridization [3][4][5]. Many powdery mildew resistance genes are derived from wild relatives of wheat, and have been introduced to common wheat by additions, substitutions, and translocations of chromosomes.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Evaluation of Resistance to Powdery Mildew of Wheat–Aegilops geniculata Roth 7Mg (7A) Alien Disomic Substitution Line W16998

Wang

Long

Wang

et al. 2020

IJMS

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Aegilops geniculata Roth has been used as a donor of disease-resistance genes, to enrich the gene pool for wheat (Triticum aestivum) improvement through distant hybridization. In this study, the wheat–Ae. geniculata alien disomic substitution line W16998 was obtained from the BC1F8 progeny of a cross between the common wheat ‘Chinese Spring’ (CS) and Ae. geniculata Roth (serial number: SY159//CS). This line was identified using cytogenetic techniques, analysis of genomic in situ hybridization (GISH), functional molecular markers (Expressed sequence tag-sequence-tagged site (EST–STS) and PCR-based landmark unique gene (PLUG), fluorescence in situ hybridization (FISH), sequential fluorescence in situ hybridization–genomic in situ hybridization (sequential FISH–GISH), and assessment of agronomic traits and powdery mildew resistance. During the anaphase of meiosis, these were evenly distributed on both sides of the equatorial plate, and they exhibited high cytological stability during the meiotic metaphase and anaphase. GISH analysis indicated that W16998 contained a pair of Ae. geniculata alien chromosomes and 40 common wheat chromosomes. One EST–STS marker and seven PLUG marker results showed that the introduced chromosomes of Ae. geniculata belonged to homoeologous group 7. Nullisomic–tetrasomic analyses suggested that the common wheat chromosome, 7A, was absent in W16998. FISH and sequential FISH–GISH analyses confirmed that the introduced Ae. geniculata chromosome was 7Mg. Therefore, W16998 was a wheat–Ae. geniculata 7Mg (7A) alien disomic substitution line. Inoculation of isolate E09 (Blumeria graminis f. sp. tritici) in the seedling stage showed that SY159 and W16998 were resistant to powdery mildew, indeed nearly immune, whereas CS was highly susceptible. Compared to CS, W16998 exhibited increased grain weight and more spikelets, and a greater number of superior agronomic traits. Consequently, W16998 was potentially useful. Germplasms transfer new disease-resistance genes and prominent agronomic traits into common wheat, giving the latter some fine properties for breeding.

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Molecular cytogenetic identification of a wheat–Aegilops geniculata Roth 7Mg disomic addition line with powdery mildew resistance

Cited by 29 publications

References 36 publications

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Characterization and Evaluation of Resistance to Powdery Mildew of Wheat–Aegilops geniculata Roth 7Mg (7A) Alien Disomic Substitution Line W16998

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