Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust

Li, Mingzhu; Wang, Yanzhen; Liu, Xiaojuan; Li, Xingfeng; Wang, Honggang; Bao, Yinguang

doi:10.3389/fpls.2021.727734

Cited by 14 publications

(7 citation statements)

References 32 publications

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“…The elite cultivars, such as Xiaoyan 6, Gaoyou 503, Xiaoyan 60, and Xiaoyan 81, were widely planted in China and had played an important role in wheat production (Li et al, 2015;Luo et al, 2021;Yang et al, 2022). Meanwhile, numerous chromosome engineering materials were generated, including partial amphiploids (Zheng et al, 2015), additions (Li et al, 2016), substitutions (Wang et al, 2019;Li et al, 2021), translocations (Yang et al, 2022) and introgression lines (Zhan et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Two Wheat-Thinopyrum ponticum Introgression Lines With Pyramiding Resistance to Powdery Mildew

Yuan

et al. 2022

Front. Plant Sci.

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Powdery mildew is one of the most devastating foliar diseases in wheat production. The wild relative Thinopyrum ponticum (2n = 10x = 70) has been widely used in wheat genetic improvement due to its superior resistance to both biotic and abiotic stresses. In the present study, two wheat-Th. ponticum introgression lines named SN0293-2 and SN0293-7 were developed from the progenies of a cross between the octoploid Trititrigia SNTE20 and common wheat, including the elite cultivar Jimai 22. They had a novel powdery mildew resistance gene (temporarily named PmSN0293) putatively from Th. ponticum pyramided with Pm2 and Pm52, exhibiting excellent Pm resistance at both the seedling and adult stages. Sequential GISH-FISH detected no signal of Th. ponticum in these two lines but a pair of T1BL·1RS in SN0293-2. Chromosomal structural variations were also observed obviously in SN0293-2 and SN0293-7. Through the Wheat 660K SNP array, 157 SNPs, 134 of which were on 6A, were found to be specific to Th. ponticum. Based on the data combined with DNA re-sequencing, seven specific markers, including one CAPS marker on 2B and six CAPS and Indel markers on 6A, were developed, confirming their wheat-Th. ponticum introgression nature. Furthermore, the two lines displayed positive plant height and produced more kernels and higher 1,000-grain weight. Excellent resistance with desirable agronomic traits makes them valuable in wheat breeding programs.

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Section: Discussionmentioning

confidence: 99%

Characterization of Two Wheat-Thinopyrum ponticum Introgression Lines With Pyramiding Resistance to Powdery Mildew

Yuan

et al. 2022

Front. Plant Sci.

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“…In situ hybridization is a visual and accurate technique that is widely used in wheat distant hybridization ( Metzlaff et al., 1986 ; Rayburn and Gill, 1987 ; Yang et al., 2015 ; Zhang et al., 2017a ; Li et al., 2021 ). Genomic DNA of L. mollis and P. huashanica were usually used as GISH probe to identify the introduced L. mollis chromosome in wheat background ( Yang et al., 2014a ; Zhang et al., 2017a ; Feng et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight

Feng²,

Li³

et al. 2022

Front. Plant Sci.

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Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) potentially harbours useful genes that might contribute to the improvement of wheat. We describe M862 as a novel wheat-L. mollis alien disomic substitution line from a cross between wheat cv. 7182 and octoploid Tritileymus M47. Cytological observations indicate that M862 has a chromosome constitution of 2n = 42 = 21II. Two 4D chromosomes of wheat substituted by two L. mollis Ns chromosomes were observed, using the GISH and ND-FISH analyses. Molecular marker, 55K SNP array and wheat-P. huashanica liquid array (GenoBaits®WheatplusPh) analyses further indicate that the alien chromosomes are L. mollis 4Ns. Therefore, it was deduced that M862 was a wheat-L. mollis 4Ns(4D) alien disomic substitution line. There were also changes in chromosomes 1A, 1D, 2B and 5A detected by ND-FISH analysis. Transcriptome sequencing showed that the structural variation of 1D, 1A and 5A may have smaller impact on gene expression than that for 2B. In addition, a total of 16 markers derived from Lm#4Ns were developed from transcriptome sequences, and these proved to be highly effective for tracking the introduced chromosome. M862 showed reduced height, larger grains (weight and width), and was highly resistance to CYR32 and CYR34 stripe rust races at the seedling stage and mixed stripe rust races (CYR32, CYR33 and CYR34) at the adult stage. It was also resistance to Fusarium head blight (FHB). This alien disomic substitution line M862 may be exploited as an important genetic material in the domestication of stipe rust and FHB resistance wheat varieties.

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“…[40]. Wheat distant hybridization is a long-standing and increasingly important method to improve the genetic diversity of common wheat by introducing bene cial genes from related species of wheat [22,41]. Using distant hybridization and conventional breeding, Wang et al have introduced Fhb7 into diverse wheat backgrounds for wheat improvement in wheat and was validated by genomic in situ hybridization [42].…”

Section: Discussionmentioning

confidence: 99%

“…The relative plant of wheat, living in the natural environment for a long time, has been tested by all kinds of terrible environmental conditions, and has a lot of excellent genes and traits which were not possessed or have been lost in common wheat. Introduction of elite genes from wheat relatives into common wheat that is an important way to increase wheat genetic diversity, broaden the genetic basis of wheat breeding and improve wheat varieties [22][23][24][25]. In 2002, Zeller et al found the powdery mildew resistance gene Pm29 in Ae.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of resistance to powdery mildew on 7Mg chromosome of Wheat–Aegilops geniculata,development and utilization of specific molecular markers

Wang

Fan

Xiao

et al. 2022

Preprint

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Background: Blumeria graminis f. sp. Tritici (Bgt) is prevalent in the main wheat-producing regions of China and result in serious yield losses in recent years. Breeding resistant cultivars is the most environmentally sound measure of disease control. Aegilops geniculata Roth, a close relative of common wheat, is an important and valuable disease resistance gene donor for wheat improvement.Results: In this study, to validate powdery mildew resistance on chromosome 7Mg, two genetic populations were constructed and analyzed. Wheat – Ae. geniculata 7Mg disomic addition line and 7Mg (7A) alien disomic substitution line crossed with susceptible Yuanfeng175 of susceptible powdery mildew respectively to form generations F1 and F2. Cytological examination, in situ hybridization (ISH), and functional molecular markers analysis showed that alien chromosomes could be inherited stably, produce different gamete types and enrich the intermediate materials for wheat genetic variation. The populations were inoculated with the physiological race E09 of powdery mildew at seedling stage. The results revealed that the plants showed high resistance to powdery mildew with chromosome 7Mg. Besides, more specific markers were developed to verify chromosome 7Mg resistance based on SLAF-seq technique. Then, 84 specific molecular markers were obtained about chromosome 7Mg. Among them, four markers were selected randomly to checked in two genetic populations. In summary, the above analysis confirmed that a dominant high powdery mildew resistance gene inherited were located on the chromosome 7Mg of Aegilops geniculate. Conclusions: The results provide a basis for resistance gene mapping and specific marker development in future.

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Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust

Cited by 14 publications

References 32 publications

Characterization of Two Wheat-Thinopyrum ponticum Introgression Lines With Pyramiding Resistance to Powdery Mildew

Characterization of Two Wheat-Thinopyrum ponticum Introgression Lines With Pyramiding Resistance to Powdery Mildew

Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight

Genetic analysis of resistance to powdery mildew on 7Mg chromosome of Wheat–Aegilops geniculata,development and utilization of specific molecular markers

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